Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse
Acquisition of specific neuronal identity by individual brain nuclei is a key step in brain development. However, how the mechanisms that confer neuronal identity are integrated with upstream regional specification networks is still mysterious. Expression of Sonic hedgehog (Shh), is required for hyp...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
14 August 2015
|
| In: |
Frontiers in neuroanatomy
Year: 2015, Jahrgang: 9 |
| ISSN: | 1662-5129 |
| DOI: | 10.3389/fnana.2015.00113 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.3389/fnana.2015.00113 Verlag: https://www.frontiersin.org/articles/10.3389/fnana.2015.00113/full 
|
| Verfasserangaben: | Michael Heide, Yuanfeng Zhang, Xunlei Zhou, Tianyu Zhao, Amaya Miquelajauregui, Alfredo Varela-Echavarria, Gonzalo Alvarez-Bolado |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1726993582 | ||
| 003 | DE-627 | ||
| 005 | 20220818174251.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200813s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fnana.2015.00113 |2 doi | |
| 035 | |a (DE-627)1726993582 | ||
| 035 | |a (DE-599)KXP1726993582 | ||
| 035 | |a (OCoLC)1341355209 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Heide, Michael |e VerfasserIn |0 (DE-588)1054055505 |0 (DE-627)791016943 |0 (DE-576)409978124 |4 aut | |
| 245 | 1 | 0 | |a Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse |c Michael Heide, Yuanfeng Zhang, Xunlei Zhou, Tianyu Zhao, Amaya Miquelajauregui, Alfredo Varela-Echavarria, Gonzalo Alvarez-Bolado |
| 264 | 1 | |c 14 August 2015 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 13.08.2020 | ||
| 520 | |a Acquisition of specific neuronal identity by individual brain nuclei is a key step in brain development. However, how the mechanisms that confer neuronal identity are integrated with upstream regional specification networks is still mysterious. Expression of Sonic hedgehog (Shh), is required for hypothalamic specification and is later downregulated by Tbx3 to allow for the differentiation of the tubero-mamillary region. In this region, the mamillary body (MBO), is a large neuronal aggregate essential for memory formation. To clarify how MBO identity is acquired after regional specification, we investigated Lhx5, a transcription factor with restricted MBO expression. We first generated a hypomorph allele of Lhx5—in homozygotes, the MBO disappears after initial specification. Intriguingly, in these mutants, Tbx3 was downregulated and the Shh expression domain abnormally extended. Microarray analysis and chromatin immunoprecipitation indicated that Lhx5 appears to be involved in Shh downregulation through Tbx3 and activates several MBO-specific regulator and effector genes. Finally, by tracing the caudal hypothalamic cell lineage we show that, in the Lhx5 mutant, at least some MBO cells are present but lack characteristic marker expression. Our work shows how the Lhx5 locus contributes to integrate regional specification pathways with downstream acquisition of neuronal identity in the MBO. | ||
| 650 | 4 | |a knockout | |
| 650 | 4 | |a LIM-HD | |
| 650 | 4 | |a mammillary body | |
| 650 | 4 | |a Mutant mice | |
| 650 | 4 | |a Neuronal identity | |
| 650 | 4 | |a Sonic Hedgehog (Shh) | |
| 700 | 1 | |a Zhang, Yuanfeng |e VerfasserIn |0 (DE-588)1072831511 |0 (DE-627)828394873 |0 (DE-576)434274828 |4 aut | |
| 700 | 1 | |a Zhou, Xunlei |e VerfasserIn |0 (DE-588)1072657015 |0 (DE-627)827961197 |0 (DE-576)434162345 |4 aut | |
| 700 | 1 | |a Zhao, Tianyu |e VerfasserIn |4 aut | |
| 700 | 1 | |a Miquelajauregui, Amaya |e VerfasserIn |4 aut | |
| 700 | 1 | |a Varela-Echavarria, Alfredo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Alvarez-Bolado, Gonzalo |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in neuroanatomy |d Lausanne : Frontiers Research Foundation, 2007 |g 9(2015) Artikel-Nummer 113, 15 Seiten |h Online-Ressource |w (DE-627)579826465 |w (DE-600)2452969-2 |w (DE-576)286502283 |x 1662-5129 |7 nnas |a Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse |
| 773 | 1 | 8 | |g volume:9 |g year:2015 |g extent:15 |a Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse |
| 856 | 4 | 0 | |u https://doi.org/10.3389/fnana.2015.00113 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.frontiersin.org/articles/10.3389/fnana.2015.00113/full |x Verlag |
| 951 | |a AR | ||
| 992 | |a 20200813 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 1072657015 |a Zhou, Xunlei |m 1072657015:Zhou, Xunlei |d 50000 |d 53100 |e 50000PZ1072657015 |e 53100PZ1072657015 |k 0/50000/ |k 1/50000/53100/ |p 3 | ||
| 998 | |g 1072831511 |a Zhang, Yuanfeng |m 1072831511:Zhang, Yuanfeng |d 50000 |d 53100 |e 50000PZ1072831511 |e 53100PZ1072831511 |k 0/50000/ |k 1/50000/53100/ |p 2 | ||
| 998 | |g 1054055505 |a Heide, Michael |m 1054055505:Heide, Michael |d 50000 |d 53100 |e 50000PH1054055505 |e 53100PH1054055505 |k 0/50000/ |k 1/50000/53100/ |p 1 |x j | ||
| 999 | |a KXP-PPN1726993582 |e 3739296305 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["1726993582"],"doi":["10.3389/fnana.2015.00113"]},"title":[{"title":"Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse","title_sort":"Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouse"}],"recId":"1726993582","note":["Gesehen am 13.08.2020"],"person":[{"roleDisplay":"VerfasserIn","family":"Heide","display":"Heide, Michael","role":"aut","given":"Michael"},{"roleDisplay":"VerfasserIn","family":"Zhang","display":"Zhang, Yuanfeng","role":"aut","given":"Yuanfeng"},{"display":"Zhou, Xunlei","role":"aut","given":"Xunlei","family":"Zhou","roleDisplay":"VerfasserIn"},{"family":"Zhao","roleDisplay":"VerfasserIn","role":"aut","display":"Zhao, Tianyu","given":"Tianyu"},{"roleDisplay":"VerfasserIn","family":"Miquelajauregui","given":"Amaya","display":"Miquelajauregui, Amaya","role":"aut"},{"role":"aut","display":"Varela-Echavarria, Alfredo","given":"Alfredo","roleDisplay":"VerfasserIn","family":"Varela-Echavarria"},{"given":"Gonzalo","display":"Alvarez-Bolado, Gonzalo","role":"aut","family":"Alvarez-Bolado","roleDisplay":"VerfasserIn"}],"physDesc":[{"extent":"15 S."}],"name":{"displayForm":["Michael Heide, Yuanfeng Zhang, Xunlei Zhou, Tianyu Zhao, Amaya Miquelajauregui, Alfredo Varela-Echavarria, Gonzalo Alvarez-Bolado"]},"relHost":[{"recId":"579826465","pubHistory":["2007 -"],"note":["Gesehen am 04.06.20"],"disp":"Lhx5 controls mamillary differentiation in the developing hypothalamus of the mouseFrontiers in neuroanatomy","title":[{"title":"Frontiers in neuroanatomy","title_sort":"Frontiers in neuroanatomy"}],"id":{"zdb":["2452969-2"],"eki":["579826465"],"issn":["1662-5129"]},"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"origin":[{"publisherPlace":"Lausanne","dateIssuedKey":"2007","dateIssuedDisp":"2007-","publisher":"Frontiers Research Foundation"}],"part":{"text":"9(2015) Artikel-Nummer 113, 15 Seiten","volume":"9","extent":"15","year":"2015"},"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["Frontiers Research Foundation"]}}],"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"14 August 2015"}]} | ||
| SRT | |a HEIDEMICHALHX5CONTRO1420 | ||