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Protease-resistant streptavidin for interaction proteomics

Abstract Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners; however, intense streptavidin-derived peptides impede protein identification by mass spectrometry. Here, we present an approach to chemically modify streptavidin, th...

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Main Authors: Rafiee, Mahmoud-Reza (Author) , Sigismondo, Gianluca (Author) , Kalxdorf, Mathias (Author) , Förster, Laura-Christin (Author) , Brügger, Britta (Author) , Béthune, Julien (Author) , Krijgsveld, Jeroen (Author)
Format: Article (Journal)
Language:English
Published: June 2020
In: Molecular systems biology
Year: 2020, Volume: 16, Issue: 5
ISSN:1744-4292
DOI:10.15252/msb.20199370
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.15252/msb.20199370
Verlag, lizenzpflichtig, Volltext: https://www.embopress.org/doi/full/10.15252/msb.20199370
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Author Notes:Mahmoud-Reza Rafiee, Gianluca Sigismondo, Mathias Kalxdorf, Laura Förster, Britta Brügger, Julien Béthune, & Jeroen Krijgsveld

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