Bioartificial heart: a human-sized porcine model : the way ahead
Background: A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Native hearts decellularized with preserved architecture and vasculature may provide an acellular tissue platform for organ regeneration. We sought to develop a tissue-engineered...
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| Hauptverfasser: | , , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
November 3, 2014
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| In: |
PLOS ONE
Year: 2014, Jahrgang: 9, Heft: 11 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0111591 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0111591 Verlag, lizenzpflichtig, Volltext: https://dx.plos.org/10.1371/journal.pone.0111591 
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| Verfasserangaben: | Alexander Weymann, Nikhil Prakash Patil, Anton Sabashnikov, Philipp Jungebluth, Sevil Korkmaz, Shiliang Li, Gabor Veres, Pal Soos, Roland Ishtok, Nicole Chaimow, Ines Pätzold, Natalie Czerny, Carsten Schies, Bastian Schmack, Aron-Frederik Popov, André Rüdiger Simon, Matthias Karck, Gabor Szabo |
| Zusammenfassung: | Background: A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Native hearts decellularized with preserved architecture and vasculature may provide an acellular tissue platform for organ regeneration. We sought to develop a tissue-engineered whole-heart neoscaffold in human-sized porcine hearts. - Methods: We decellularized porcine hearts (n = 10) by coronary perfusion with ionic detergents in a modified Langendorff circuit. We confirmed decellularization by histology, transmission electron microscopy and fluorescence microscopy, quantified residual DNA by spectrophotometry, and evaluated biomechanical stability with ex-vivo left-ventricular pressure/ volume studies, all compared to controls. We then mounted the decellularized porcine hearts in a bioreactor and reseeded them with murine neonatal cardiac cells and human umbilical cord derived endothelial cells (HUVEC) under simulated physiological conditions. - Results: Decellularized hearts lacked intracellular components but retained specific collagen fibers, proteoglycan, elastin and mechanical integrity; quantitative DNA analysis demonstrated a significant reduction of DNA compared to controls (82.663.2 ng DNA/mg tissue vs. 473.2613.4 ng DNA/mg tissue, p,0.05). Recellularized porcine whole-heart neoscaffolds demonstrated re-endothelialization of coronary vasculature and measurable intrinsic myocardial electrical activity at 10 days, with perfused organ culture maintained for up to 3 weeks. - Conclusions: Human-sized decellularized porcine hearts provide a promising tissue-engineering platform that may lead to future clinical strategies in the treatment of heart failure. |
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| Beschreibung: | Gesehen am 18.08.2020 |
| Beschreibung: | Online Resource |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0111591 |