Proteomics of vitreous humor of patients with exudative age-related macular degeneration
Background There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD). Methods and Findings Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idio...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
May 14, 2014
|
| In: |
PLOS ONE
Year: 2014, Jahrgang: 9, Heft: 5 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0096895 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1371/journal.pone.0096895 Verlag, lizenzpflichtig, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096895 
|
| Verfasserangaben: | Michael Janusz Koss, Janosch Hoffmann, Nauke Nguyen, Marcel Pfister, Harald Mischak, William Mullen, Holger Husi, Robert Rejdak, Frank Koch, Joachim Jankowski, Katharina Krueger, Thomas Bertelmann, Julie Klein, Joost P. Schanstra, Justyna Siwy |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1727456378 | ||
| 003 | DE-627 | ||
| 005 | 20241225023806.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200819s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0096895 |2 doi | |
| 035 | |a (DE-627)1727456378 | ||
| 035 | |a (DE-599)KXP1727456378 | ||
| 035 | |a (OCoLC)1341355929 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Koss, Michael Janusz |d 1979- |e VerfasserIn |0 (DE-588)131446819 |0 (DE-627)509448690 |0 (DE-576)298483521 |4 aut | |
| 245 | 1 | 0 | |a Proteomics of vitreous humor of patients with exudative age-related macular degeneration |c Michael Janusz Koss, Janosch Hoffmann, Nauke Nguyen, Marcel Pfister, Harald Mischak, William Mullen, Holger Husi, Robert Rejdak, Frank Koch, Joachim Jankowski, Katharina Krueger, Thomas Bertelmann, Julie Klein, Joost P. Schanstra, Justyna Siwy |
| 264 | 1 | |c May 14, 2014 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 19.08.2020 | ||
| 520 | |a Background There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD). Methods and Findings Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idiopathic floaters) were analyzed with capillary electrophoresis coupled to mass spectrometry in this retrospective case series to define potential candidate protein markers of AMD. Nineteen proteins were found to be upregulated in vitreous of AMD patients. Most of the proteins were plasma derived and involved in biological (ion) transport, acute phase inflammatory reaction, and blood coagulation. A number of proteins have not been previously associated to AMD including alpha-1-antitrypsin, fibrinogen alpha chain and prostaglandin H2-D isomerase. Alpha-1-antitrypsin was validated in vitreous of an independent set of AMD patients using Western blot analysis. Further systems biology analysis of the data indicated that the observed proteomic changes may reflect upregulation of immune response and complement activity. Conclusions Proteome analysis of vitreous samples from patients with AMD, which underwent an intravitreal combination therapy including a core vitrectomy, steroids and bevacizumab, revealed apparent AMD-specific proteomic changes. The identified AMD-associated proteins provide some insight into the pathophysiological changes associated with AMD. | ||
| 650 | 4 | |a Acute phase proteins | |
| 650 | 4 | |a Apolipoproteins | |
| 650 | 4 | |a Biological transport | |
| 650 | 4 | |a Biomarkers | |
| 650 | 4 | |a Collagens | |
| 650 | 4 | |a Cytoskeletal proteins | |
| 650 | 4 | |a Macular degeneration | |
| 650 | 4 | |a Serum proteins | |
| 700 | 1 | |a Hoffmann, Janosch |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nguyen, Nauke |e VerfasserIn |4 aut | |
| 700 | 1 | |8 1\p |a Pfister, Marcel |d 1979- |e VerfasserIn |0 (DE-588)1033451967 |0 (DE-627)741245663 |0 (DE-576)381054470 |4 aut | |
| 700 | 1 | |a Mischak, Harald |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mullen, William |e VerfasserIn |4 aut | |
| 700 | 1 | |a Husi, Holger |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rejdak, Robert |e VerfasserIn |4 aut | |
| 700 | 1 | |a Koch, Frank |e VerfasserIn |4 aut | |
| 700 | 1 | |8 2\p |a Jankowski, Joachim |e VerfasserIn |0 (DE-588)1037235401 |0 (DE-627)755318447 |0 (DE-576)391253255 |4 aut | |
| 700 | 1 | |8 3\p |a Krueger, Katharina |d 1975- |e VerfasserIn |0 (DE-588)1017301107 |0 (DE-627)676295304 |0 (DE-576)353470252 |4 aut | |
| 700 | 1 | |8 4\p |a Bertelmann, Thomas |d 1978- |e VerfasserIn |0 (DE-588)138573069 |0 (DE-627)604371047 |0 (DE-576)308117255 |4 aut | |
| 700 | 1 | |a Klein, Julie |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schanstra, Joost P. |e VerfasserIn |4 aut | |
| 700 | 1 | |8 5\p |a Siwy, Justyna |d 1983- |e VerfasserIn |0 (DE-588)1076337309 |0 (DE-627)834720345 |0 (DE-576)445215437 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 9(2014,5) Artikel-Nummer e96895, 11 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a Proteomics of vitreous humor of patients with exudative age-related macular degeneration |
| 773 | 1 | 8 | |g volume:9 |g year:2014 |g number:5 |g extent:11 |a Proteomics of vitreous humor of patients with exudative age-related macular degeneration |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.pone.0096895 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096895 |x Verlag |z lizenzpflichtig |3 Volltext |
| 883 | |8 1\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 2\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 3\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 4\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 883 | |8 5\p |a cgwrk |d 20241001 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | ||
| 951 | |a AR | ||
| 992 | |a 20200819 | ||
| 993 | |a Article | ||
| 994 | |a 2014 | ||
| 998 | |g 131446819 |a Koss, Michael Janusz |m 131446819:Koss, Michael Janusz |d 910000 |d 910900 |e 910000PK131446819 |e 910900PK131446819 |k 0/910000/ |k 1/910000/910900/ |p 1 |x j | ||
| 999 | |a KXP-PPN1727456378 |e 3741194913 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"doi":["10.1371/journal.pone.0096895"],"eki":["1727456378"]},"origin":[{"dateIssuedDisp":"May 14, 2014","dateIssuedKey":"2014"}],"name":{"displayForm":["Michael Janusz Koss, Janosch Hoffmann, Nauke Nguyen, Marcel Pfister, Harald Mischak, William Mullen, Holger Husi, Robert Rejdak, Frank Koch, Joachim Jankowski, Katharina Krueger, Thomas Bertelmann, Julie Klein, Joost P. Schanstra, Justyna Siwy"]},"relHost":[{"pubHistory":["1.2006 -"],"part":{"text":"9(2014,5) Artikel-Nummer e96895, 11 Seiten","volume":"9","extent":"11","year":"2014","issue":"5"},"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Proteomics of vitreous humor of patients with exudative age-related macular degenerationPLOS ONE","note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"recId":"523574592","corporate":[{"role":"isb","display":"Public Library of Science","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"title":[{"title":"PLOS ONE","title_sort":"PLOS ONE"}],"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"dateIssuedDisp":"2006-","publisher":"PLOS ; PLoS","dateIssuedKey":"2006","publisherPlace":"San Francisco, California, US ; Lawrence, Kan."}],"id":{"issn":["1932-6203"],"zdb":["2267670-3"],"eki":["523574592"]},"name":{"displayForm":["Public Library of Science"]}}],"physDesc":[{"extent":"11 S."}],"title":[{"title_sort":"Proteomics of vitreous humor of patients with exudative age-related macular degeneration","title":"Proteomics of vitreous humor of patients with exudative age-related macular degeneration"}],"person":[{"family":"Koss","given":"Michael Janusz","display":"Koss, Michael Janusz","roleDisplay":"VerfasserIn","role":"aut"},{"family":"Hoffmann","given":"Janosch","roleDisplay":"VerfasserIn","display":"Hoffmann, Janosch","role":"aut"},{"role":"aut","display":"Nguyen, Nauke","roleDisplay":"VerfasserIn","given":"Nauke","family":"Nguyen"},{"family":"Pfister","given":"Marcel","display":"Pfister, Marcel","roleDisplay":"VerfasserIn","role":"aut"},{"given":"Harald","family":"Mischak","role":"aut","roleDisplay":"VerfasserIn","display":"Mischak, Harald"},{"given":"William","family":"Mullen","role":"aut","roleDisplay":"VerfasserIn","display":"Mullen, William"},{"display":"Husi, Holger","roleDisplay":"VerfasserIn","role":"aut","family":"Husi","given":"Holger"},{"given":"Robert","family":"Rejdak","role":"aut","roleDisplay":"VerfasserIn","display":"Rejdak, Robert"},{"roleDisplay":"VerfasserIn","display":"Koch, Frank","role":"aut","family":"Koch","given":"Frank"},{"family":"Jankowski","given":"Joachim","display":"Jankowski, Joachim","roleDisplay":"VerfasserIn","role":"aut"},{"roleDisplay":"VerfasserIn","display":"Krueger, Katharina","role":"aut","family":"Krueger","given":"Katharina"},{"given":"Thomas","family":"Bertelmann","role":"aut","roleDisplay":"VerfasserIn","display":"Bertelmann, Thomas"},{"family":"Klein","given":"Julie","display":"Klein, Julie","roleDisplay":"VerfasserIn","role":"aut"},{"display":"Schanstra, Joost P.","roleDisplay":"VerfasserIn","role":"aut","family":"Schanstra","given":"Joost P."},{"given":"Justyna","family":"Siwy","role":"aut","roleDisplay":"VerfasserIn","display":"Siwy, Justyna"}],"language":["eng"],"recId":"1727456378","note":["Gesehen am 19.08.2020"],"type":{"media":"Online-Ressource","bibl":"article-journal"}} | ||
| SRT | |a KOSSMICHAEPROTEOMICS1420 | ||