Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infections
Background: Transplantation of human corneal tissue is associated with the potential risk of transmittance of viral infections. In accordance with European directives and federal laws, in Germany each tissue donor has to be tested for infectious diseases such as hepatitis B and C virus (HBV and HCV)...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2020
|
| In: |
Transfusion medicine and hemotherapy
Year: 2019, Volume: 47, Issue: 3, Pages: 236-243 |
| ISSN: | 1660-3818 |
| DOI: | 10.1159/000502866 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1159/000502866 Verlag, lizenzpflichtig, Volltext: https://www.karger.com/Article/FullText/502866 
|
| Author Notes: | Ingo Schmack, Seda Ballikaya, Brigitte Erber, Irina Voehringer, Ulrich Burkhardt, Gerd U. Auffarth, Paul Schnitzler |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1727519744 | ||
| 003 | DE-627 | ||
| 005 | 20230426130554.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200820r20202019xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1159/000502866 |2 doi | |
| 035 | |a (DE-627)1727519744 | ||
| 035 | |a (DE-599)KXP1727519744 | ||
| 035 | |a (OCoLC)1341356663 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Schmack, Ingo |d 1970- |e VerfasserIn |0 (DE-588)123840333 |0 (DE-627)70644633X |0 (DE-576)293903026 |4 aut | |
| 245 | 1 | 0 | |a Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infections |c Ingo Schmack, Seda Ballikaya, Brigitte Erber, Irina Voehringer, Ulrich Burkhardt, Gerd U. Auffarth, Paul Schnitzler |
| 264 | 1 | |c 2020 | |
| 300 | |a 8 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published online: September 24, 2019 | ||
| 500 | |a Gesehen am 20.08.2020 | ||
| 520 | |a Background: Transplantation of human corneal tissue is associated with the potential risk of transmittance of viral infections. In accordance with European directives and federal laws, in Germany each tissue donor has to be tested for infectious diseases such as hepatitis B and C virus (HBV and HCV) and human immunodeficiency virus (HIV) infection. However, most of the currently available CE-marked serologic and nucleic acid screening systems are only validated for antemortem blood. Methods: Twenty related and paired ante- and postmortem blood samples from cornea donors were obtained and subsequently analyzed for hepatitis B surface antigen (HBsAg), hepatitis B antibody (anti-HBc), anti-HCV, HCV RNA, anti-HIV-1/2, and HIV p24 Ag using Abbott test systems. The sera were also spiked with reference materials in concentrations giving low and high positivity for HBV, HCV, and HIV markers. Results: The spiked ante- and postmortem sera from related donors showed similar results for HBsAg, anti-HBc, anti-HCV, HCV RNA, anti-HIV, and HIV p24 Ag, indicating a high stability of viral markers in cadaveric specimens. Three cornea donors had a medical history of HBV infection and revealed anti-HBc at similar levels in the ante- and postmortem sera. In addition, there was a single postmortem sample demonstrating a weak signal of anti-HIV-1 and HIV-1 p24 Ag. False-positive or false-negative results were not detected. The results obtained with the Abbott ARCHITECT analyzer and Abbott RealTime HCV PCR showed no significant differences. Conclusion: The analyzed screening assays are suitable for the detection of infectious markers of HBV, HCV, and HIV at similar levels in spiked ante- and postmortem sera from cornea donors. | ||
| 534 | |c 2019 | ||
| 700 | 1 | |a Ballikaya, Seda |e VerfasserIn |0 (DE-588)1033922064 |0 (DE-627)743671767 |0 (DE-576)381600513 |4 aut | |
| 700 | 1 | |a Erber, Brigitte |e VerfasserIn |0 (DE-588)1216263388 |0 (DE-627)1727519264 |4 aut | |
| 700 | 1 | |a Burkhardt, Ulrich |e VerfasserIn |0 (DE-588)1072323214 |0 (DE-627)827144318 |0 (DE-576)433774452 |4 aut | |
| 700 | 1 | |a Auffarth, Gerd U. |d 1964- |e VerfasserIn |0 (DE-588)123385261 |0 (DE-627)577130420 |0 (DE-576)293687277 |4 aut | |
| 700 | 1 | |a Schnitzler, Paul |d 1959- |e VerfasserIn |0 (DE-588)1022905643 |0 (DE-627)717329798 |0 (DE-576)366155695 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Transfusion medicine and hemotherapy |d Basel : Karger, 2003 |g 47(2020), 3, Seite 236-243 |h Online-Ressource |w (DE-627)360670695 |w (DE-600)2100533-3 |w (DE-576)11277542X |x 1660-3818 |7 nnas |a Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infections |
| 773 | 1 | 8 | |g volume:47 |g year:2020 |g number:3 |g pages:236-243 |g extent:8 |a Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infections |
| 856 | 4 | 0 | |u https://doi.org/10.1159/000502866 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.karger.com/Article/FullText/502866 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200820 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1022905643 |a Schnitzler, Paul |m 1022905643:Schnitzler, Paul |d 910000 |d 911700 |d 50000 |e 910000PS1022905643 |e 911700PS1022905643 |e 50000PS1022905643 |k 0/910000/ |k 1/910000/911700/ |k 0/50000/ |p 7 |y j | ||
| 998 | |g 123385261 |a Auffarth, Gerd U. |m 123385261:Auffarth, Gerd U. |d 910000 |d 910900 |e 910000PA123385261 |e 910900PA123385261 |k 0/910000/ |k 1/910000/910900/ |p 6 | ||
| 998 | |g 1072323214 |a Burkhardt, Ulrich |m 1072323214:Burkhardt, Ulrich |d 50000 |e 50000PB1072323214 |k 0/50000/ |p 5 | ||
| 998 | |g 1216263388 |a Erber, Brigitte |m 1216263388:Erber, Brigitte |d 50000 |e 50000PE1216263388 |k 0/50000/ |p 3 | ||
| 998 | |g 1033922064 |a Ballikaya, Seda |m 1033922064:Ballikaya, Seda |p 2 | ||
| 999 | |a KXP-PPN1727519744 |e 3741314471 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"media":"Online-Ressource","bibl":"article-journal"},"name":{"displayForm":["Ingo Schmack, Seda Ballikaya, Brigitte Erber, Irina Voehringer, Ulrich Burkhardt, Gerd U. Auffarth, Paul Schnitzler"]},"title":[{"title_sort":"Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infections","title":"Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infections"}],"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"2020"}],"person":[{"given":"Ingo","display":"Schmack, Ingo","role":"aut","family":"Schmack"},{"given":"Seda","display":"Ballikaya, Seda","role":"aut","family":"Ballikaya"},{"given":"Brigitte","family":"Erber","display":"Erber, Brigitte","role":"aut"},{"family":"Burkhardt","display":"Burkhardt, Ulrich","role":"aut","given":"Ulrich"},{"given":"Gerd U.","display":"Auffarth, Gerd U.","role":"aut","family":"Auffarth"},{"display":"Schnitzler, Paul","role":"aut","family":"Schnitzler","given":"Paul"}],"id":{"doi":["10.1159/000502866"],"eki":["1727519744"]},"note":["Published online: September 24, 2019","Gesehen am 20.08.2020"],"language":["eng"],"relHost":[{"pubHistory":["30.2003 -"],"language":["eng","ger"],"origin":[{"dateIssuedDisp":"2003-","publisherPlace":"Basel","dateIssuedKey":"2003","publisher":"Karger"}],"id":{"zdb":["2100533-3"],"eki":["360670695"],"issn":["1660-3818"]},"part":{"pages":"236-243","text":"47(2020), 3, Seite 236-243","year":"2020","volume":"47","extent":"8","issue":"3"},"disp":"Validation of spiked postmortem blood samples from cornea donors on the abbott ARCHITECT and m2000 systems for viral infectionsTransfusion medicine and hemotherapy","title":[{"subtitle":"interdisciplinary journal for transfusion medicine, hemotherapy, immunohematology, and clinical hemostatis","title":"Transfusion medicine and hemotherapy","title_sort":"Transfusion medicine and hemotherapy"}],"name":{"displayForm":["official organ of German Society for Transfusion Medicine and Immunohematology"]},"recId":"360670695","physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 04.05.2023","Fortsetzung der Druck-Ausgabe","Ungezählte Beil.: Supplement"],"titleAlt":[{"title":"offizielles Organ der Deutschen Gesellschaft für Transfusionsmedizin und Immunhämatologie ; interdisziplinäre Zeitschrift für Infusions- und Hämotherapie, Immunhämatologie und klinische Hämostaseologie"}],"type":{"bibl":"periodical","media":"Online-Ressource"}}],"recId":"1727519744","physDesc":[{"extent":"8 S."}]} | ||
| SRT | |a SCHMACKINGVALIDATION2020 | ||