Buchumschlag

Functional loss of a noncanonical BCOR-PRC1.1 complex accelerates SHH-driven medulloblastoma formation

Medulloblastoma is a malignant childhood brain tumor arising from the developing cerebellum. In Sonic Hedgehog (SHH) subgroup medulloblastoma, aberrant activation of SHH signaling causes increased proliferation of granule neuron progenitors (GNPs), and predisposes these cells to tumorigenesis. A sec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Kutscher, Lena (VerfasserIn) , Okonechnikov, Konstantin (VerfasserIn) , Batora, Nadja (VerfasserIn) , Clark, Jessica (VerfasserIn) , Silva, Patricia B. G. (VerfasserIn) , Vouri, Mikaella (VerfasserIn) , Rijn, Sjoerd van (VerfasserIn) , Sieber, Laura (VerfasserIn) , Statz, Britta (VerfasserIn) , Gearhart, Micah D. (VerfasserIn) , Shiraishi, Ryo (VerfasserIn) , Mack, Norman (VerfasserIn) , Orr, Brent A. (VerfasserIn) , Korshunov, Andrey (VerfasserIn) , Gudenas, Brian L. (VerfasserIn) , Smith, Kyle S. (VerfasserIn) , Mercier, Audrey L. (VerfasserIn) , Ayrault, Olivier (VerfasserIn) , Hoshino, Mikio (VerfasserIn) , Kool, Marcel (VerfasserIn) , Hoff, Katja von (VerfasserIn) , Graf, Norbert (VerfasserIn) , Fleishhack, Gudrun (VerfasserIn) , Bardwell, Vivian J. (VerfasserIn) , Pfister, Stefan (VerfasserIn) , Northcott, Paul A. (VerfasserIn) , Kawauchi, Daisuke (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: August 20, 2020
In: Genes & development
Year: 2020, Jahrgang: 34, Heft: 17/18, Pages: 1161-1176
ISSN:1549-5477
DOI:10.1101/gad.337584.120
Online-Zugang:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1101/gad.337584.120
Verlag, lizenzpflichtig, Volltext: http://genesdev.cshlp.org/content/early/2020/08/19/gad.337584.120
Volltext
Verfasserangaben:Lena M. Kutscher, Konstantin Okonechnikov, Nadja V. Batora, Jessica Clark, Patricia B. G. Silva, Mikaella Vouri, Sjoerd van Rijn, Laura Sieber, Britta Statz, Micah D. Gearhart, Ryo Shiraishi, Norman Mack, Brent A. Orr, Andrey Korshunov, Brian L. Gudenas, Kyle S. Smith, Audrey L. Mercier, Olivier Ayrault, Mikio Hoshino, Marcel Kool, Katja von Hoff, Norbert Graf, Gudrun Fleishhack, Vivian J. Bardwell, Stefan M. Pfister, Paul A. Northcott, and Daisuke Kawauchi
Beschreibung
Zusammenfassung:Medulloblastoma is a malignant childhood brain tumor arising from the developing cerebellum. In Sonic Hedgehog (SHH) subgroup medulloblastoma, aberrant activation of SHH signaling causes increased proliferation of granule neuron progenitors (GNPs), and predisposes these cells to tumorigenesis. A second, cooperating genetic hit is often required to push these hyperplastic cells to malignancy and confer mutation-specific characteristics associated with oncogenic signaling. Somatic loss-of-function mutations of the transcriptional corepressor BCOR are recurrent and enriched in SHH medulloblastoma. To investigate BCOR as a putative tumor suppressor, we used a genetically engineered mouse model to delete exons 9/10 of Bcor (BcorΔE9-10) in GNPs during development. This mutation leads to reduced expression of C-terminally truncated BCOR (BCORΔE9-10). While BcorΔE9-10 alone did not promote tumorigenesis or affect GNP differentiation, BcorΔE9-10 combined with loss of the SHH receptor gene Ptch1 resulted in fully penetrant medulloblastomas. In Ptch1+/−;BcorΔE9-10 tumors, the growth factor gene Igf2 was aberrantly up-regulated, and ectopic Igf2 overexpression was sufficient to drive tumorigenesis in Ptch1+/− GNPs. BCOR directly regulates Igf2, likely through the PRC1.1 complex; the repressive histone mark H2AK119Ub is decreased at the Igf2 promoter in Ptch1+/−;BcorΔE9-10 tumors. Overall, our data suggests that BCOR-PRC1.1 disruption leads to Igf2 overexpression, which transforms preneoplastic cells to malignant tumors.
Beschreibung:Gesehen am 21.08.2020
Lena M. Kutscher, Konstantin Okonechnikov and Nadja V. Batora contributed equally
Beschreibung:Online Resource
ISSN:1549-5477
DOI:10.1101/gad.337584.120

Ähnliche Einträge