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Fly-FUCCI: a versatile tool for studying cell proliferation in complex tissues

One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI...

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Main Authors: Zielke, Norman (Author) , Korzelius, Jerome (Author) , Straaten, Monique van (Author) , Bender, Katharina (Author) , Schuhknecht, Gregor F. P. (Author) , Dutta, Devanjali (Author) , Xiang, Jinyi (Author) , Edgar, Bruce (Author)
Format: Article (Journal)
Language:English
Published: April 10, 2014
In: Cell reports
Year: 2014, Volume: 7, Issue: 2, Pages: 588-598
ISSN:2211-1247
DOI:10.1016/j.celrep.2014.03.020
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2014.03.020
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124714001983
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Author Notes:Norman Zielke, Jerome Korzelius, Monique van Straaten, Katharina Bender, Gregor F.P. Schuhknecht, Devanjali Dutta, Jinyi Xiang, and Bruce A. Edgar
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Summary:One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI relies on fluorochrome-tagged degrons from the Cyclin B and E2F1 proteins, which are degraded by the ubiquitin E3-ligases APC/C and CRL4Cdt2, during mitosis or the onset of S phase, respectively. These probes can track cell-cycle patterns in cultured Drosophila cells, eye and wing imaginal discs, salivary glands, the adult midgut, and probably other tissues. To support a broad range of experimental applications, we have generated a toolkit of transgenic Drosophila lines that express the Fly-FUCCI probes under control of the UASt, UASp, QUAS, and ubiquitin promoters. The Fly-FUCCI system should be a valuable tool for visualizing cell-cycle activity during development, tissue homeostasis, and neoplastic growth.
Item Description:Gesehen am 26.08.2020
Physical Description:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2014.03.020

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