Bacterial lipopolysaccharides form physically cross-linked, two-dimensional gels in the presence of divalent cations
We established a bacterial membrane model with monolayers of bacterial lipopolysaccharides (LPS Re and LPS Ra) and quantified their viscoelastic properties by using an interfacial stress rheometer coupled to a Langmuir film balance. LPS Re monolayers exhibited purely viscous behaviour in the absence...
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| Hauptverfasser: | , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
02 Jul 2015
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| In: |
Soft matter
Year: 2015, Jahrgang: 11, Heft: 30, Pages: 6037-6044 |
| ISSN: | 1744-6848 |
| DOI: | 10.1039/C5SM01002K |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1039/C5SM01002K Verlag: https://pubs.rsc.org/en/content/articlelanding/2015/sm/c5sm01002k 
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| Verfasserangaben: | Moritz Herrmann, Emanuel Schneck, Thomas Gutsmann, Klaus Brandenburg, Motomu Tanaka |
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| 520 | |a We established a bacterial membrane model with monolayers of bacterial lipopolysaccharides (LPS Re and LPS Ra) and quantified their viscoelastic properties by using an interfacial stress rheometer coupled to a Langmuir film balance. LPS Re monolayers exhibited purely viscous behaviour in the absence of calcium ions, while the same monolayers underwent a viscous-to-elastic transition upon compression in the presence of Ca2+. Our results demonstrated for the first time that LPSs in bacterial outer membranes can form two-dimensional elastic networks in the presence of Ca2+. Different from LPS Re monolayers, the LPS Ra monolayers showed a very similar rheological transition both in the presence and absence of Ca2+, suggesting that longer saccharide chains can form 2D physical gels even in the absence of Ca2+. By exposure of the monolayers to the antimicrobial peptide protamine, we could directly monitor the differences in resistance of bacterial membranes according to the presence of calcium. | ||
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