Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length
Pumilio domain RNA-binding proteins are known mainly as posttranscriptional repressors of gene expression that reduce mRNA translation and stability. Trypanosoma brucei has 11 PUF proteins. We show here that PUF2 is in the cytosol, with roughly the same number of molecules per cell as there are mRNA...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
28 March 2014
|
| In: |
Eukaryotic cell
Year: 2014, Jahrgang: 13, Heft: 5, Pages: 664-674 |
| ISSN: | 1535-9786 |
| DOI: | 10.1128/EC.00018-14 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/EC.00018-14 Verlag, lizenzpflichtig, Volltext: https://ec.asm.org/content/13/5/664 
|
| Verfasserangaben: | Bhaskar Anand Jha, Abeer Fadda, Clementine Merce, Elisha Mugo, Dorothea Droll, Christine Clayton |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1728639301 | ||
| 003 | DE-627 | ||
| 005 | 20220818190533.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200902s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1128/EC.00018-14 |2 doi | |
| 035 | |a (DE-627)1728639301 | ||
| 035 | |a (DE-599)KXP1728639301 | ||
| 035 | |a (OCoLC)1341358139 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Jha, Bhaskar Anand |e VerfasserIn |0 (DE-588)1033022683 |0 (DE-627)739965816 |0 (DE-576)380503522 |4 aut | |
| 245 | 1 | 0 | |a Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length |c Bhaskar Anand Jha, Abeer Fadda, Clementine Merce, Elisha Mugo, Dorothea Droll, Christine Clayton |
| 264 | 1 | |c 28 March 2014 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.09.2020 | ||
| 520 | |a Pumilio domain RNA-binding proteins are known mainly as posttranscriptional repressors of gene expression that reduce mRNA translation and stability. Trypanosoma brucei has 11 PUF proteins. We show here that PUF2 is in the cytosol, with roughly the same number of molecules per cell as there are mRNAs. Although PUF2 exhibits a low level of in vivo RNA binding, it is not associated with polysomes. PUF2 also decreased reporter mRNA levels in a tethering assay, consistent with a repressive role. Depletion of PUF2 inhibited growth of bloodstream-form trypanosomes, causing selective loss of mRNAs with long open reading frames and increases in mRNAs with shorter open reading frames. Reexamination of published RNASeq data revealed the same trend in cells depleted of some other proteins. We speculate that these length effects could be caused by inhibition of the elongation phase of transcription or by an influence of translation status or polysomal conformation on mRNA decay. | ||
| 700 | 1 | |a Fadda, Abeer |e VerfasserIn |0 (DE-588)1156723353 |0 (DE-627)1019664959 |0 (DE-576)502396938 |4 aut | |
| 700 | 1 | |a Mercé, Clémentine |e VerfasserIn |0 (DE-588)1098446852 |0 (DE-627)857632833 |0 (DE-576)469125101 |4 aut | |
| 700 | 1 | |a Mugo, Elisha Muchunga |e VerfasserIn |0 (DE-588)1138933651 |0 (DE-627)89677354X |0 (DE-576)492767831 |4 aut | |
| 700 | 1 | |a Droll, Dorothea |d 19XX- |e VerfasserIn |0 (DE-588)1027191436 |0 (DE-627)728556510 |0 (DE-576)372575803 |4 aut | |
| 700 | 1 | |a Clayton, Christine |e VerfasserIn |0 (DE-588)1098447158 |0 (DE-627)85763335X |0 (DE-576)469125624 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Eukaryotic cell |d Washington, DC : American Society for Microbiology, 2002 |g 13(2014), 5, Seite 664-674 |h Online-Ressource |w (DE-627)342319957 |w (DE-600)2071564-X |w (DE-576)097268194 |x 1535-9786 |7 nnas |a Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length |
| 773 | 1 | 8 | |g volume:13 |g year:2014 |g number:5 |g pages:664-674 |g extent:11 |a Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length |
| 856 | 4 | 0 | |u https://doi.org/10.1128/EC.00018-14 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://ec.asm.org/content/13/5/664 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200902 | ||
| 993 | |a Article | ||
| 994 | |a 2014 | ||
| 998 | |g 1098447158 |a Clayton, Christine |m 1098447158:Clayton, Christine |d 700000 |d 706000 |e 700000PC1098447158 |e 706000PC1098447158 |k 0/700000/ |k 1/700000/706000/ |p 6 |y j | ||
| 998 | |g 1027191436 |a Droll, Dorothea |m 1027191436:Droll, Dorothea |d 700000 |d 706000 |e 700000PD1027191436 |e 706000PD1027191436 |k 0/700000/ |k 1/700000/706000/ |p 5 | ||
| 998 | |g 1138933651 |a Mugo, Elisha Muchunga |m 1138933651:Mugo, Elisha Muchunga |p 4 | ||
| 998 | |g 1098446852 |a Mercé, Clémentine |m 1098446852:Mercé, Clémentine |d 700000 |d 706000 |e 700000PM1098446852 |e 706000PM1098446852 |k 0/700000/ |k 1/700000/706000/ |p 3 | ||
| 998 | |g 1156723353 |a Fadda, Abeer |m 1156723353:Fadda, Abeer |p 2 | ||
| 998 | |g 1033022683 |a Jha, Bhaskar Anand |m 1033022683:Jha, Bhaskar Anand |p 1 |x j | ||
| 999 | |a KXP-PPN1728639301 |e 3746101107 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"language":["eng"],"recId":"1728639301","note":["Gesehen am 02.09.2020"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"person":[{"family":"Jha","given":"Bhaskar Anand","roleDisplay":"VerfasserIn","display":"Jha, Bhaskar Anand","role":"aut"},{"role":"aut","display":"Fadda, Abeer","roleDisplay":"VerfasserIn","given":"Abeer","family":"Fadda"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Mercé, Clémentine","given":"Clémentine","family":"Mercé"},{"roleDisplay":"VerfasserIn","display":"Mugo, Elisha Muchunga","role":"aut","family":"Mugo","given":"Elisha Muchunga"},{"family":"Droll","given":"Dorothea","roleDisplay":"VerfasserIn","display":"Droll, Dorothea","role":"aut"},{"role":"aut","roleDisplay":"VerfasserIn","display":"Clayton, Christine","given":"Christine","family":"Clayton"}],"title":[{"title_sort":"Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length","title":"Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length"}],"relHost":[{"name":{"displayForm":["American Society for Microbiology"]},"origin":[{"publisherPlace":"Washington, DC","dateIssuedDisp":"2002-2015","dateIssuedKey":"2002","publisher":"American Society for Microbiology"}],"id":{"issn":["1535-9786"],"zdb":["2071564-X"],"eki":["342319957"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Eukaryotic cell","title":"Eukaryotic cell"}],"disp":"Depletion of the trypanosome pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region lengthEukaryotic cell","type":{"bibl":"periodical","media":"Online-Ressource"},"note":["Gesehen am 15.06.21"],"language":["eng"],"corporate":[{"role":"isb","display":"American Society for Microbiology","roleDisplay":"Herausgebendes Organ"}],"recId":"342319957","pubHistory":["1.2002-volume 14, number 12 (December 2015)"],"part":{"extent":"11","text":"13(2014), 5, Seite 664-674","volume":"13","issue":"5","pages":"664-674","year":"2014"}}],"physDesc":[{"extent":"11 S."}],"name":{"displayForm":["Bhaskar Anand Jha, Abeer Fadda, Clementine Merce, Elisha Mugo, Dorothea Droll, Christine Clayton"]},"id":{"doi":["10.1128/EC.00018-14"],"eki":["1728639301"]},"origin":[{"dateIssuedDisp":"28 March 2014","dateIssuedKey":"2014"}]} | ||
| SRT | |a JHABHASKARDEPLETIONO2820 | ||