rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish
  name:
    name part: Auer, Thomas
    role:
      role term: creator
    role:
      role term: VerfasserIn
    role:
      role term: aut
  name:
    name part: Del Bene, Filippo
    role:
      role term: VerfasserIn
    role:
      role term: aut
  type of resource: text
  origin info:
    place:
      place term: xx
    date issued: 2014
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: Illustrationen
    extent: 9
  abstract: The targeted introduction of mutations utilizing sequence specific transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (RNA-guided nucleases, RGNs) has revolutionized reverse genetic approaches in numerous model organisms. In zebrafish, both systems were successfully applied to generate loss-of-function alleles by targeting open reading frames or deletion and inversion of whole chromosomal regions. In addition to the production of these loss-of-function alleles, genomic engineering by insertion of short sequences utilizing single stranded DNA oligonucleotides as templates for homology based repair was made possible, enabling effective insertion of loxP sites or tags for protein coding genes. Recent studies based on homologous recombination and non-homologous end joining have also broadened the repertoire for genome editing. These approaches allow the targeted insertion of open reading frames or even whole donor vectors. In this review we summarize the use of TALENs and RNA-guided nucleases in the field of zebrafish genetics with a special focus on knock-in approaches.
  note: Thomas O. Auer, Filippo Del Bene
  note: Gesehen am 04.09.2020
  subject:
    topic: CRISPR/Cas9
  subject:
    topic: Homologous recombination
  subject:
    topic: Homology independent repair
  subject:
    topic: TALENs
  subject:
    topic: Zebrafish
  classification: 32
  related item:
    title info:
      title: Methods
    name:
      name part: CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish
    origin info:
      publisher: Orlando, Fla. : Academic Press, 1990
    physical description:
      extent: Online-Ressource
    identifier: 1095-9130
    identifier: (DE-627)26784008X
    identifier: (DE-600)1471152-7
    identifier: (DE-576)104193972
    part:
      text: 69(2014), 2, Seite 142-150
  related item:
    name:
      name part: CRISPR/Cas9 and TALEN-mediated knock-in approaches in zebrafish
    part:
      text: volume:69
    part:
      text: year:2014
    part:
      text: number:2
    part:
      text: pages:142-150
    part:
      text: extent:9
  identifier: https://doi.org/10.1016/j.ymeth.2014.03.027
  identifier: http://www.sciencedirect.com/science/article/pii/S1046202314001297
  record info:
    record content source: DE-627
    record creation date: 200904
    record change date: 20220818191858.0
    record identifier: 1728902886
    language of cataloging:
      language term: ger