Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymoma
Ependymoma is a heterogeneous entity of central nervous system tumors with well-established molecular groups. Here, we apply single-cell RNA sequencing to analyze ependymomas across molecular groups and anatomic locations to investigate their intratumoral heterogeneity and developmental origins. Epe...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 13, 2020
|
| In: |
Cancer cell
Year: 2020, Jahrgang: 38, Heft: 1, Pages: 44-59.e9 |
| ISSN: | 1878-3686 |
| DOI: | 10.1016/j.ccell.2020.06.004 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.ccell.2020.06.004 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S1535610820303081 
|
| Verfasserangaben: | Johannes Gojo, Bernhard Englinger, Li Jiang, Jens M. Hübner, McKenzie L. Shaw, Olivia A. Hack, Sibylle Madlener, Dominik Kirchhofer, Ilon Liu, Jason Pyrdol, Volker Hovestadt, Emanuele Mazzola, Nathan D. Mathewson, Maria Trissal, Daniela Lötsch, Christian Dorfer, Christine Haberler, Angela Halfmann, Lisa Mayr, Andreas Peyrl, Rene Geyeregger, Benjamin Schwalm, Monica Mauermann, Kristian W. Pajtler, Till Milde, Marni E. Shore, Jack E. Geduldig, Kristine Pelton, Thomas Czech, Orr Ashenberg, Kai W. Wucherpfennig, Orit Rozenblatt-Rosen, Sanda Alexandrescu, Keith L. Ligon, Stefan M. Pfister, Aviv Regev, Irene Slavc, Walter Berger, Mario L. Suvà, Marcel Kool, and Mariella G. Filbin |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1729091865 | ||
| 003 | DE-627 | ||
| 005 | 20220818193004.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200908s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ccell.2020.06.004 |2 doi | |
| 035 | |a (DE-627)1729091865 | ||
| 035 | |a (DE-599)KXP1729091865 | ||
| 035 | |a (OCoLC)1341359015 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Gojo, Johannes |e VerfasserIn |0 (DE-627)158109681X |0 (DE-576)51109681X |4 aut | |
| 245 | 1 | 0 | |a Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymoma |c Johannes Gojo, Bernhard Englinger, Li Jiang, Jens M. Hübner, McKenzie L. Shaw, Olivia A. Hack, Sibylle Madlener, Dominik Kirchhofer, Ilon Liu, Jason Pyrdol, Volker Hovestadt, Emanuele Mazzola, Nathan D. Mathewson, Maria Trissal, Daniela Lötsch, Christian Dorfer, Christine Haberler, Angela Halfmann, Lisa Mayr, Andreas Peyrl, Rene Geyeregger, Benjamin Schwalm, Monica Mauermann, Kristian W. Pajtler, Till Milde, Marni E. Shore, Jack E. Geduldig, Kristine Pelton, Thomas Czech, Orr Ashenberg, Kai W. Wucherpfennig, Orit Rozenblatt-Rosen, Sanda Alexandrescu, Keith L. Ligon, Stefan M. Pfister, Aviv Regev, Irene Slavc, Walter Berger, Mario L. Suvà, Marcel Kool, and Mariella G. Filbin |
| 264 | 1 | |c July 13, 2020 | |
| 300 | |a 25 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 08.09.2020 | ||
| 520 | |a Ependymoma is a heterogeneous entity of central nervous system tumors with well-established molecular groups. Here, we apply single-cell RNA sequencing to analyze ependymomas across molecular groups and anatomic locations to investigate their intratumoral heterogeneity and developmental origins. Ependymomas are composed of a cellular hierarchy initiating from undifferentiated populations, which undergo impaired differentiation toward three lineages of neuronal-glial fate specification. While prognostically favorable groups of ependymoma predominantly harbor differentiated cells, aggressive groups are enriched for undifferentiated cell populations. The delineated transcriptomic signatures correlate with patient survival and define molecular dependencies for targeted treatment approaches. Taken together, our analyses reveal a developmental hierarchy underlying ependymomas relevant to biological and clinical behavior. | ||
| 650 | 4 | |a aberrant development | |
| 650 | 4 | |a cancer stem cells | |
| 650 | 4 | |a cellular hierarchy | |
| 650 | 4 | |a differentiation trajectory | |
| 650 | 4 | |a EPN | |
| 650 | 4 | |a glial development | |
| 650 | 4 | |a intratumoral heterogeneity | |
| 650 | 4 | |a Pediatric ependymoma | |
| 650 | 4 | |a single-cell RNA sequencing | |
| 650 | 4 | |a target identification | |
| 700 | 1 | |a Hübner, Jens-Martin |e VerfasserIn |0 (DE-588)1170916848 |0 (DE-627)1040230024 |0 (DE-576)512708568 |4 aut | |
| 700 | 1 | |a Pajtler, Kristian Wilfried |d 1980- |e VerfasserIn |0 (DE-588)1012877264 |0 (DE-627)662768558 |0 (DE-576)346086256 |4 aut | |
| 700 | 1 | |a Milde, Till |d 1973- |e VerfasserIn |0 (DE-588)1038311837 |0 (DE-627)75711234X |0 (DE-576)39229334X |4 aut | |
| 700 | 1 | |a Pfister, Stefan |d 1974- |e VerfasserIn |0 (DE-588)123850215 |0 (DE-627)706450930 |0 (DE-576)293908400 |4 aut | |
| 700 | 1 | |a Kool, Marcel |e VerfasserIn |0 (DE-588)1058669753 |0 (DE-627)797393560 |0 (DE-576)414823842 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cancer cell |d Cambridge, Mass. : Cell Press, 2002 |g 38(2020), 1, Seite 44-59.e9 |h Online-Ressource |w (DE-627)343970082 |w (DE-600)2074034-7 |w (DE-576)097935972 |x 1878-3686 |7 nnas |a Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymoma |
| 773 | 1 | 8 | |g volume:38 |g year:2020 |g number:1 |g pages:44-59.e9 |g extent:25 |a Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymoma |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.ccell.2020.06.004 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S1535610820303081 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200908 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1058669753 |a Kool, Marcel |m 1058669753:Kool, Marcel |d 50000 |e 50000PK1058669753 |k 0/50000/ |p 40 | ||
| 998 | |g 123850215 |a Pfister, Stefan |m 123850215:Pfister, Stefan |d 910000 |d 910500 |e 910000PP123850215 |e 910500PP123850215 |k 0/910000/ |k 1/910000/910500/ |p 35 | ||
| 998 | |g 1038311837 |a Milde, Till |m 1038311837:Milde, Till |d 910000 |d 910500 |e 910000PM1038311837 |e 910500PM1038311837 |k 0/910000/ |k 1/910000/910500/ |p 25 | ||
| 998 | |g 1012877264 |a Pajtler, Kristian Wilfried |m 1012877264:Pajtler, Kristian Wilfried |d 910000 |d 910500 |e 910000PP1012877264 |e 910500PP1012877264 |k 0/910000/ |k 1/910000/910500/ |p 24 | ||
| 999 | |a KXP-PPN1729091865 |e 3747571964 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title_sort":"Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymoma","title":"Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymoma"}],"person":[{"role":"aut","given":"Johannes","display":"Gojo, Johannes","family":"Gojo","roleDisplay":"VerfasserIn"},{"display":"Hübner, Jens-Martin","family":"Hübner","roleDisplay":"VerfasserIn","role":"aut","given":"Jens-Martin"},{"roleDisplay":"VerfasserIn","family":"Pajtler","display":"Pajtler, Kristian Wilfried","given":"Kristian Wilfried","role":"aut"},{"display":"Milde, Till","family":"Milde","roleDisplay":"VerfasserIn","role":"aut","given":"Till"},{"role":"aut","given":"Stefan","display":"Pfister, Stefan","family":"Pfister","roleDisplay":"VerfasserIn"},{"display":"Kool, Marcel","family":"Kool","roleDisplay":"VerfasserIn","role":"aut","given":"Marcel"}],"note":["Gesehen am 08.09.2020"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"25 S."}],"language":["eng"],"id":{"doi":["10.1016/j.ccell.2020.06.004"],"eki":["1729091865"]},"recId":"1729091865","relHost":[{"note":["Gesehen am 28.02.11"],"part":{"volume":"38","pages":"44-59.e9","year":"2020","text":"38(2020), 1, Seite 44-59.e9","extent":"25","issue":"1"},"title":[{"title":"Cancer cell","title_sort":"Cancer cell"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"id":{"issn":["1878-3686"],"zdb":["2074034-7"],"eki":["343970082"]},"language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"recId":"343970082","origin":[{"publisherPlace":"Cambridge, Mass. ; New York, NY","dateIssuedKey":"2002","dateIssuedDisp":"2002-","publisher":"Cell Press ; Elsevier"}],"pubHistory":["1.2002 -"],"disp":"Single-cell RNA-seq reveals cellular hierarchies and impaired developmental trajectories in pediatric ependymomaCancer cell"}],"name":{"displayForm":["Johannes Gojo, Bernhard Englinger, Li Jiang, Jens M. Hübner, McKenzie L. Shaw, Olivia A. Hack, Sibylle Madlener, Dominik Kirchhofer, Ilon Liu, Jason Pyrdol, Volker Hovestadt, Emanuele Mazzola, Nathan D. Mathewson, Maria Trissal, Daniela Lötsch, Christian Dorfer, Christine Haberler, Angela Halfmann, Lisa Mayr, Andreas Peyrl, Rene Geyeregger, Benjamin Schwalm, Monica Mauermann, Kristian W. Pajtler, Till Milde, Marni E. Shore, Jack E. Geduldig, Kristine Pelton, Thomas Czech, Orr Ashenberg, Kai W. Wucherpfennig, Orit Rozenblatt-Rosen, Sanda Alexandrescu, Keith L. Ligon, Stefan M. Pfister, Aviv Regev, Irene Slavc, Walter Berger, Mario L. Suvà, Marcel Kool, and Mariella G. Filbin"]},"origin":[{"dateIssuedDisp":"July 13, 2020","dateIssuedKey":"2020"}]} | ||
| SRT | |a GOJOJOHANNSINGLECELL1320 | ||