Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification
The identification of optimal process parameters for the isolation of a target component from multicomponent mixtures is especially challenging in industrial applications. With constantly increasing time-to-market pressure, screening a large parameter space is not feasible and design-of-experiment a...
Gespeichert in:
| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2016
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| In: |
Engineering in life sciences
Year: 2015, Jahrgang: 16, Heft: 2, Pages: 107-113 |
| ISSN: | 1618-2863 |
| DOI: | 10.1002/elsc.201400248 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/elsc.201400248 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/elsc.201400248 
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| Verfasserangaben: | Tobias Hahn, Thiemo Huuk, Anna Osberghaus, Katharina Doninger, Susanne Nath, Stefan Hepbildikler, Vincent Heuveline, Jürgen Hubbuch |
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| 245 | 1 | 0 | |a Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification |c Tobias Hahn, Thiemo Huuk, Anna Osberghaus, Katharina Doninger, Susanne Nath, Stefan Hepbildikler, Vincent Heuveline, Jürgen Hubbuch |
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| 520 | |a The identification of optimal process parameters for the isolation of a target component from multicomponent mixtures is especially challenging in industrial applications. With constantly increasing time-to-market pressure, screening a large parameter space is not feasible and design-of-experiment approaches with few experiments might fail due to dynamic and nonlinear reactions to small parameter changes. Model-based optimization can determine optimal operating conditions, once the model has been calibrated to the specific process step. In this work, parameters for the steric mass action model were estimated for the target protein and three impurities of an industrial antibody cation-exchange purification step using only chromatograms at different wavelengths and additional fraction analyses with size exclusion chromatography. Information on the molar or mass concentrations in the feed are not available. The model-based optimization results coincide with conventional chromatogram-based optimization. | ||
| 534 | |c 2015 | ||
| 650 | 4 | |a Industrial application | |
| 650 | 4 | |a Inverse method | |
| 650 | 4 | |a Liquid chromatography | |
| 650 | 4 | |a Mechanistic modeling | |
| 650 | 4 | |a Optimization | |
| 700 | 1 | |a Huuk, Thiemo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Osberghaus, Anna |e VerfasserIn |4 aut | |
| 700 | 1 | |a Doninger, Katharina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Nath, Susanne |e VerfasserIn |4 aut | |
| 700 | 1 | |a Hepbildikler, Stefan |e VerfasserIn |4 aut | |
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| 700 | 1 | |a Hubbuch, Jürgen |e VerfasserIn |4 aut | |
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