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Critical role of type III interferon in controlling SARS-CoV-2 infection in human intestinal epithelial cells

Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) is an unprecedented worldwide health problem that requires concerted and global approaches to stop the coronavirus 2019 (COVID-19) pandemic. Although SARS-CoV-2 primarily targets lung epithelium cells, there is growing evidence tha...

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Main Authors: Stanifer, Megan (Author) , Kee, Carmon (Author) , Cortese, Mirko (Author) , Metz Zumarán, Camila (Author) , Triana, Sergio (Author) , Mukenhirn, Markus (Author) , Kräusslich, Hans-Georg (Author) , Alexandrov, Theodore (Author) , Bartenschlager, Ralf (Author) , Boulant, Steeve (Author)
Format: Article (Journal)
Language:English
Published: 19 June 2020
In: Cell reports
Year: 2020, Volume: 32, Issue: 1
ISSN:2211-1247
DOI:10.1016/j.celrep.2020.107863
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2020.107863
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124720308445
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Author Notes:Megan L. Stanifer, Carmon Kee, Mirko Cortese, Camila Metz Zumaran, Sergio Triana, Markus Mukenhirn, Hans-Georg Kraeusslich, Theodore Alexandrov, Ralf Bartenschlager, and Steeve Boulant
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Summary:Severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) is an unprecedented worldwide health problem that requires concerted and global approaches to stop the coronavirus 2019 (COVID-19) pandemic. Although SARS-CoV-2 primarily targets lung epithelium cells, there is growing evidence that the intestinal epithelium is also infected. Here, using both colon-derived cell lines and primary non-transformed colon organoids, we engage in the first comprehensive analysis of the SARS-CoV-2 life cycle in human intestinal epithelial cells (hIECs). Our results demonstrate that hIECs fully support SARS-CoV-2 infection, replication, and production of infectious de novo virus particles. We found that viral infection elicits an extremely robust intrinsic immune response where interferon-mediated responses are efficient at controlling SARS-CoV-2 replication and de novo virus production. Taken together, our data demonstrate that hIECs are a productive site of SARS-CoV-2 replication and suggest that the enteric phase of SARS-CoV-2 may participate in the pathologies observed in COVID-19 patients by contributing to increasing patient viremia and fueling an exacerbated cytokine response.
Item Description:Gesehen am 14.09.2020
Physical Description:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2020.107863

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