Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7
Bone morphogenic proteins (BMPs) promote the survival of neurons, suggesting a therapeutic application of BMPs in the treatment of acute and chronic neurodegenerative disorders. However, the application of recombinant BMPs in vivo is limited by their short half-life. To provide a continuous supply f...
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| Main Authors: | , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
11 March 2014
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| In: |
BMC neuroscience
Year: 2014, Volume: 15, Issue: 1 |
| ISSN: | 1471-2202 |
| DOI: | 10.1186/1471-2202-15-38 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1186/1471-2202-15-38 Verlag, lizenzpflichtig, Volltext: https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-15-38 |
| Author Notes: | Ann-Marie Heinonen, Mahbubur Rahman, Godwin Dogbevia, Hannah Jakobi, Stefan Wölfl, Rolf Sprengel and Markus Schwaninger |
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| 520 | |a Bone morphogenic proteins (BMPs) promote the survival of neurons, suggesting a therapeutic application of BMPs in the treatment of acute and chronic neurodegenerative disorders. However, the application of recombinant BMPs in vivo is limited by their short half-life. To provide a continuous supply for functionally active BMPs, we expressed BMP7, BMP2 and the BMP inhibitor Noggin under the control of rAAV vectors in vivo. For visual control of rAAV-mediated BMP (v-BMP) expression we fused the secreted morphogenic polypeptides and the fluorescent reporter protein Venus via the ‘ribosomal skip’ promoting 2A peptide-bridge. | ||
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