Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assay
The third-generation tyrosine kinase inhibitor (TKI), osimertinib, has revolutionized the treatment of patients with non-small cell lung carcinoma with epidermal growth factor receptor (EGFR)-activating mutation, and resistant to first- and second-generation TKIs. Osimertinib is now also proposed as...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
14 July 2020
|
| In: |
Cancers
Year: 2020, Volume: 12, Issue: 7 |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers12071897 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cancers12071897
|
| Author Notes: | Margaux Fresnais, André Roth, Kathrin I. Foerster, Dirk Jäger, Stefan M. Pfister, Walter E. Haefeli, Jürgen Burhenne and Rémi Longuespée |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1733565574 | ||
| 003 | DE-627 | ||
| 005 | 20230426163433.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200922s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cancers12071897 |2 doi | |
| 035 | |a (DE-627)1733565574 | ||
| 035 | |a (DE-599)KXP1733565574 | ||
| 035 | |a (OCoLC)1341361117 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Fresnais, Margaux |e VerfasserIn |0 (DE-588)1190043157 |0 (DE-627)1668720108 |4 aut | |
| 245 | 1 | 0 | |a Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assay |c Margaux Fresnais, André Roth, Kathrin I. Foerster, Dirk Jäger, Stefan M. Pfister, Walter E. Haefeli, Jürgen Burhenne and Rémi Longuespée |
| 264 | 1 | |c 14 July 2020 | |
| 300 | |a 18 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.09.2020 | ||
| 520 | |a The third-generation tyrosine kinase inhibitor (TKI), osimertinib, has revolutionized the treatment of patients with non-small cell lung carcinoma with epidermal growth factor receptor (EGFR)-activating mutation, and resistant to first- and second-generation TKIs. Osimertinib is now also proposed as a first-line therapy, thus extending the scope of applications in lung oncology. Personalized medicine approaches are still necessary to monitor if patients are exposed to adequate concentrations of osimertinib during their treatment. It would also help to understand the appearance of new resistances in patients after several months of dosing with osimertinib. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is currently the gold standard for the quantification of drugs in plasma enabling pharmacokinetic analyses and patient monitoring. In the present study, we propose an alternative to LC-MS/MS methods for the rapid and sensitive quantification of osimertinib in plasma using matrix-assisted laser desorption/ionization (MALDI) -MS. The presented assay requires only 3 min per sample for their preparation, analysis, and data extraction, and less than 3 h for quantification. A lower limit of quantification (LLOQ) of 5 ng/mL in plasma was retrieved. The method was fully validated, following the guidelines of the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for bioanalytical method validation. The present developments prove the importance to consider alternative MS assays for time-efficient quantification of small molecule inhibitors in plasma in the context of personalized medicine for targeted therapies. | ||
| 650 | 4 | |a MALDI | |
| 650 | 4 | |a mass spectrometry | |
| 650 | 4 | |a osimertinib | |
| 650 | 4 | |a quantification | |
| 650 | 4 | |a tyrosine kinase inhibitors | |
| 700 | 1 | |a Roth, André |e VerfasserIn |0 (DE-588)1218115327 |0 (DE-627)1733567984 |4 aut | |
| 700 | 1 | |a Foerster, Kathrin |e VerfasserIn |0 (DE-588)118114602X |0 (DE-627)1662507089 |4 aut | |
| 700 | 1 | |a Jäger, Dirk |d 1964- |e VerfasserIn |0 (DE-588)1032507535 |0 (DE-627)738505323 |0 (DE-576)380074125 |4 aut | |
| 700 | 1 | |a Pfister, Stefan |d 1974- |e VerfasserIn |0 (DE-588)123850215 |0 (DE-627)706450930 |0 (DE-576)293908400 |4 aut | |
| 700 | 1 | |a Haefeli, Walter E. |d 1958- |e VerfasserIn |0 (DE-588)124572359 |0 (DE-627)656806141 |0 (DE-576)340514221 |4 aut | |
| 700 | 1 | |a Burhenne, Jürgen |d 1963- |e VerfasserIn |0 (DE-588)1034228889 |0 (DE-627)745126987 |0 (DE-576)381858197 |4 aut | |
| 700 | 1 | |a Longuespée, Rémi |e VerfasserIn |0 (DE-588)113707146X |0 (DE-627)89404690X |0 (DE-576)491093179 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cancers |d Basel : MDPI, 2009 |g 12(2020,7) Artikel-Nummer 1897, 18 Seiten |h Online-Ressource |w (DE-627)614095670 |w (DE-600)2527080-1 |w (DE-576)313958548 |x 2072-6694 |7 nnas |a Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assay |
| 773 | 1 | 8 | |g volume:12 |g year:2020 |g number:7 |g extent:18 |a Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assay |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cancers12071897 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200922 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 113707146X |a Longuespée, Rémi |m 113707146X:Longuespée, Rémi |d 910000 |d 910100 |e 910000PL113707146X |e 910100PL113707146X |k 0/910000/ |k 1/910000/910100/ |p 8 |y j | ||
| 998 | |g 1034228889 |a Burhenne, Jürgen |m 1034228889:Burhenne, Jürgen |d 910000 |d 910100 |e 910000PB1034228889 |e 910100PB1034228889 |k 0/910000/ |k 1/910000/910100/ |p 7 | ||
| 998 | |g 124572359 |a Haefeli, Walter E. |m 124572359:Haefeli, Walter E. |d 910000 |d 910100 |e 910000PH124572359 |e 910100PH124572359 |k 0/910000/ |k 1/910000/910100/ |p 6 | ||
| 998 | |g 123850215 |a Pfister, Stefan |m 123850215:Pfister, Stefan |d 910000 |d 910500 |e 910000PP123850215 |e 910500PP123850215 |k 0/910000/ |k 1/910000/910500/ |p 5 | ||
| 998 | |g 1032507535 |a Jäger, Dirk |m 1032507535:Jäger, Dirk |d 910000 |e 910000PJ1032507535 |k 0/910000/ |p 4 | ||
| 998 | |g 118114602X |a Foerster, Kathrin |m 118114602X:Foerster, Kathrin |d 910000 |d 910100 |e 910000PF118114602X |e 910100PF118114602X |k 0/910000/ |k 1/910000/910100/ |p 3 | ||
| 998 | |g 1218115327 |a Roth, André |m 1218115327:Roth, André |p 2 | ||
| 998 | |g 1190043157 |a Fresnais, Margaux |m 1190043157:Fresnais, Margaux |p 1 |x j | ||
| 999 | |a KXP-PPN1733565574 |e 3760394531 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"id":{"eki":["1733565574"],"doi":["10.3390/cancers12071897"]},"name":{"displayForm":["Margaux Fresnais, André Roth, Kathrin I. Foerster, Dirk Jäger, Stefan M. Pfister, Walter E. Haefeli, Jürgen Burhenne and Rémi Longuespée"]},"physDesc":[{"extent":"18 S."}],"recId":"1733565574","relHost":[{"id":{"eki":["614095670"],"zdb":["2527080-1"],"issn":["2072-6694"]},"name":{"displayForm":["Molecular Diversity Preservation International (MDPI)"]},"physDesc":[{"extent":"Online-Ressource"}],"recId":"614095670","type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assayCancers","note":["Gesehen am 27.05.2020"],"origin":[{"publisherPlace":"Basel","dateIssuedKey":"2009","publisher":"MDPI","dateIssuedDisp":"2009-"}],"pubHistory":["1.2009 -"],"title":[{"title":"Cancers","title_sort":"Cancers"}],"part":{"year":"2020","issue":"7","text":"12(2020,7) Artikel-Nummer 1897, 18 Seiten","extent":"18","volume":"12"},"language":["eng"]}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 22.09.2020"],"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"14 July 2020"}],"title":[{"title_sort":"Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assay","title":"Rapid and sensitive quantification of osimertinib in human plasma using a fully validated MALDI-IM-MS/MS assay"}],"language":["eng"],"person":[{"family":"Fresnais","display":"Fresnais, Margaux","role":"aut","given":"Margaux"},{"family":"Roth","display":"Roth, André","role":"aut","given":"André"},{"display":"Foerster, Kathrin","family":"Foerster","role":"aut","given":"Kathrin"},{"role":"aut","given":"Dirk","family":"Jäger","display":"Jäger, Dirk"},{"display":"Pfister, Stefan","family":"Pfister","role":"aut","given":"Stefan"},{"given":"Walter E.","role":"aut","display":"Haefeli, Walter E.","family":"Haefeli"},{"given":"Jürgen","role":"aut","family":"Burhenne","display":"Burhenne, Jürgen"},{"family":"Longuespée","display":"Longuespée, Rémi","role":"aut","given":"Rémi"}]} | ||
| SRT | |a FRESNAISMARAPIDANDSE1420 | ||