Cover Image

Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL

Stem cells are one of the foundational evolutionary novelties that allowed the independent emergence of multicellularity in the plant and animal lineages. In plants, the homeodomain (HD) transcription factor WUSCHEL (WUS) is essential for the maintenance of stem cells in the shoot apical meristem. W...

Full description

Saved in:
Bibliographic Details
Main Authors: Sloan, Jeremy (Author) , Hakenjos, Jana (Author) , Gebert, Michael (Author) , Ermakova, Olga (Author) , Gumiero, Andrea (Author) , Stier, Gunter (Author) , Wild, Klemens (Author) , Sinning, Irmgard (Author) , Lohmann, Jan U. (Author)
Format: Article (Journal)
Language:English
Published: 6 May 2020
In: Nature Communications
Year: 2020, Volume: 11
ISSN:2041-1723
DOI:10.1038/s41467-020-16024-y
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-020-16024-y
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-020-16024-y
Get full text
Author Notes:Jeremy Sloan, Jana P. Hakenjos, Michael Gebert, Olga Ermakova, Andrea Gumiero, Gunter Stier, Klemens Wild, Irmgard Sinning & Jan U. Lohmann

MARC

LEADER 00000caa a2200000 c 4500
001 1733619267
003 DE-627
005 20240407193249.0
007 cr uuu---uuuuu
008 200923s2020 xx |||||o 00| ||eng c
024 7 |a 10.1038/s41467-020-16024-y  |2 doi 
035 |a (DE-627)1733619267 
035 |a (DE-599)KXP1733619267 
035 |a (OCoLC)1341361317 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Sloan, Jeremy  |d 1987-  |e VerfasserIn  |0 (DE-588)1198441267  |0 (DE-627)1680819917  |4 aut 
245 1 0 |a Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL  |c Jeremy Sloan, Jana P. Hakenjos, Michael Gebert, Olga Ermakova, Andrea Gumiero, Gunter Stier, Klemens Wild, Irmgard Sinning & Jan U. Lohmann 
264 1 |c 6 May 2020 
300 |a 16 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 23.09.2020 
520 |a Stem cells are one of the foundational evolutionary novelties that allowed the independent emergence of multicellularity in the plant and animal lineages. In plants, the homeodomain (HD) transcription factor WUSCHEL (WUS) is essential for the maintenance of stem cells in the shoot apical meristem. WUS has been reported to bind to diverse DNA motifs and to act as transcriptional activator and repressor. However, the mechanisms underlying this remarkable behavior have remained unclear. Here, we quantitatively delineate WUS binding to three divergent DNA motifs and resolve the relevant structural underpinnings. We show that WUS exhibits a strong binding preference for TGAA repeat sequences, while retaining the ability to weakly bind to TAAT elements. This behavior is attributable to the formation of dimers through interactions of specific residues in the HD that stabilize WUS DNA interaction. Our results provide a mechanistic basis for dissecting WUS dependent regulatory networks in plant stem cell control. 
700 1 |a Hakenjos, Jana  |e VerfasserIn  |0 (DE-588)1160762260  |0 (DE-627)1024192318  |0 (DE-576)506175375  |4 aut 
700 1 |a Gebert, Michael  |e VerfasserIn  |0 (DE-588)1191200892  |0 (DE-627)1669609200  |4 aut 
700 1 |a Ermakova, Olga  |e VerfasserIn  |0 (DE-588)1161625399  |0 (DE-627)1024914194  |0 (DE-576)506773779  |4 aut 
700 1 |a Gumiero, Andrea  |e VerfasserIn  |0 (DE-588)1166462803  |0 (DE-627)1030437866  |0 (DE-576)510785107  |4 aut 
700 1 |a Stier, Gunter  |e VerfasserIn  |0 (DE-588)1164406019  |0 (DE-627)1028857144  |0 (DE-576)508520622  |4 aut 
700 1 |a Wild, Klemens  |e VerfasserIn  |0 (DE-588)1049291190  |0 (DE-627)781602394  |0 (DE-576)177410892  |4 aut 
700 1 |a Sinning, Irmgard  |d 1960-  |e VerfasserIn  |0 (DE-588)1027598617  |0 (DE-627)72945133X  |0 (DE-576)166290580  |4 aut 
700 1 |a Lohmann, Jan U.  |d 1971-  |e VerfasserIn  |0 (DE-588)121572315  |0 (DE-627)70554642X  |0 (DE-576)292778627  |4 aut 
773 0 8 |i Enthalten in  |t Nature Communications  |d [London] : Springer Nature, 2010  |g 11(2020) Artikel-Nummer 2223, 16 Seiten  |h Online-Ressource  |w (DE-627)626457688  |w (DE-600)2553671-0  |w (DE-576)331555905  |x 2041-1723  |7 nnas  |a Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL 
773 1 8 |g volume:11  |g year:2020  |g extent:16  |a Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL 
856 4 0 |u https://doi.org/10.1038/s41467-020-16024-y  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://www.nature.com/articles/s41467-020-16024-y  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20200923 
993 |a Article 
994 |a 2020 
998 |g 121572315  |a Lohmann, Jan U.  |m 121572315:Lohmann, Jan U.  |d 700000  |d 721000  |e 700000PL121572315  |e 721000PL121572315  |k 0/700000/  |k 1/700000/721000/  |p 9  |y j 
998 |g 1027598617  |a Sinning, Irmgard  |m 1027598617:Sinning, Irmgard  |d 700000  |d 711000  |e 700000PS1027598617  |e 711000PS1027598617  |k 0/700000/  |k 1/700000/711000/  |p 8 
998 |g 1049291190  |a Wild, Klemens  |m 1049291190:Wild, Klemens  |d 700000  |d 711000  |e 700000PW1049291190  |e 711000PW1049291190  |k 0/700000/  |k 1/700000/711000/  |p 7 
998 |g 1164406019  |a Stier, Gunter  |m 1164406019:Stier, Gunter  |d 700000  |d 711000  |e 700000PS1164406019  |e 711000PS1164406019  |k 0/700000/  |k 1/700000/711000/  |p 6 
998 |g 1166462803  |a Gumiero, Andrea  |m 1166462803:Gumiero, Andrea  |p 5 
998 |g 1161625399  |a Ermakova, Olga  |m 1161625399:Ermakova, Olga  |d 700000  |d 721000  |e 700000PE1161625399  |e 721000PE1161625399  |k 0/700000/  |k 1/700000/721000/  |p 4 
998 |g 1191200892  |a Gebert, Michael  |m 1191200892:Gebert, Michael  |d 700000  |d 721000  |e 700000PG1191200892  |e 721000PG1191200892  |k 0/700000/  |k 1/700000/721000/  |p 3 
998 |g 1160762260  |a Hakenjos, Jana  |m 1160762260:Hakenjos, Jana  |p 2 
998 |g 1198441267  |a Sloan, Jeremy  |m 1198441267:Sloan, Jeremy  |p 1  |x j 
999 |a KXP-PPN1733619267  |e 3760571506 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"given":"Jeremy","display":"Sloan, Jeremy","family":"Sloan","role":"aut"},{"given":"Jana","display":"Hakenjos, Jana","family":"Hakenjos","role":"aut"},{"family":"Gebert","role":"aut","given":"Michael","display":"Gebert, Michael"},{"family":"Ermakova","role":"aut","display":"Ermakova, Olga","given":"Olga"},{"display":"Gumiero, Andrea","given":"Andrea","family":"Gumiero","role":"aut"},{"given":"Gunter","display":"Stier, Gunter","role":"aut","family":"Stier"},{"family":"Wild","role":"aut","given":"Klemens","display":"Wild, Klemens"},{"family":"Sinning","role":"aut","display":"Sinning, Irmgard","given":"Irmgard"},{"role":"aut","family":"Lohmann","display":"Lohmann, Jan U.","given":"Jan U."}],"recId":"1733619267","note":["Gesehen am 23.09.2020"],"language":["eng"],"name":{"displayForm":["Jeremy Sloan, Jana P. Hakenjos, Michael Gebert, Olga Ermakova, Andrea Gumiero, Gunter Stier, Klemens Wild, Irmgard Sinning & Jan U. Lohmann"]},"relHost":[{"part":{"extent":"16","text":"11(2020) Artikel-Nummer 2223, 16 Seiten","volume":"11","year":"2020"},"language":["eng"],"note":["Gesehen am 13.06.24"],"pubHistory":["2010-"],"disp":"Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHELNature Communications","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"626457688","id":{"zdb":["2553671-0"],"eki":["626457688"],"issn":["2041-1723"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Nature Communications","title_sort":"Nature Communications"}],"origin":[{"publisherPlace":"[London] ; [London]","dateIssuedDisp":"[2010]-","publisher":"Springer Nature ; Nature Publishing Group UK"}]}],"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"6 May 2020"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"title":[{"title_sort":"Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL","title":"Structural basis for the complex DNA binding behavior of the plant stem cell regulator WUSCHEL"}],"physDesc":[{"extent":"16 S."}],"id":{"eki":["1733619267"],"doi":["10.1038/s41467-020-16024-y"]}} 
SRT |a SLOANJEREMSTRUCTURAL6202 

Similar Items