Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults
Viral transduced gene expression is the current standard for cell-type specific labelling and cell tacking in experimental neuroscience. To achieve widespread gene expression, a viral delivery method to neonatal rodents was introduced more than two decades ago. Most of those neonatal virus-injection...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
14 July 2020
|
| In: |
Frontiers in behavioral neuroscience
Year: 2020, Jahrgang: 14 |
| ISSN: | 1662-5153 |
| DOI: | 10.3389/fnbeh.2020.00115 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.3389/fnbeh.2020.00115 Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fnbeh.2020.00115/full 
|
| Verfasserangaben: | Wannan Tang, Uwe Zillmann and Rolf Sprengel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1733865454 | ||
| 003 | DE-627 | ||
| 005 | 20230426163456.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200928s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fnbeh.2020.00115 |2 doi | |
| 035 | |a (DE-627)1733865454 | ||
| 035 | |a (DE-599)KXP1733865454 | ||
| 035 | |a (OCoLC)1341367756 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Tang, Wannan |e VerfasserIn |0 (DE-588)138549842 |0 (DE-627)60380229X |0 (DE-576)308011198 |4 aut | |
| 245 | 1 | 0 | |a Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults |c Wannan Tang, Uwe Zillmann and Rolf Sprengel |
| 264 | 1 | |c 14 July 2020 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 28.09.2020 | ||
| 520 | |a Viral transduced gene expression is the current standard for cell-type specific labelling and cell tacking in experimental neuroscience. To achieve widespread gene expression, a viral delivery method to neonatal rodents was introduced more than two decades ago. Most of those neonatal virus-injection based gene transduction methods in mice have used deep hypothermia for anesthesia, which was reported to be associated with behavioral impairments in respective animals. To explore other options for neonatal viral applications, we applied a combination of Medetomidine, Midazolam and Fentanyl (MMF), each of which can be antagonized by a specific antagonist. Later in their adulthood, drug treated, virus injected mice and naïve mice showed a similar performance in all behavioral tasks tested, including motor coordination tasks, anxiety-related tasks and spatial memory tasks, demonstrating MMF anesthesia could be safely applied to mice for neonatal viral transduction at postnatal day 2. | ||
| 700 | 1 | |a Zillmann, Uwe |e VerfasserIn |0 (DE-588)1218491965 |0 (DE-627)1733865136 |4 aut | |
| 700 | 1 | |a Sprengel, Rolf |d 1955- |e VerfasserIn |0 (DE-588)1060395665 |0 (DE-627)799661228 |0 (DE-576)163694230 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in behavioral neuroscience |d Lausanne : Frontiers Research Foundation, 2007 |g 14(2020) Artikel-Nummer 115, 10 Seiten |h Online-Ressource |w (DE-627)579826392 |w (DE-600)2452960-6 |w (DE-576)286502224 |x 1662-5153 |7 nnas |a Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults |
| 773 | 1 | 8 | |g volume:14 |g year:2020 |g extent:10 |a Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults |
| 856 | 4 | 0 | |u https://doi.org/10.3389/fnbeh.2020.00115 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://www.frontiersin.org/articles/10.3389/fnbeh.2020.00115/full |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20200928 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1060395665 |a Sprengel, Rolf |m 1060395665:Sprengel, Rolf |p 3 |y j | ||
| 998 | |g 1218491965 |a Zillmann, Uwe |m 1218491965:Zillmann, Uwe |p 2 | ||
| 999 | |a KXP-PPN1733865454 |e 3763706755 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults","title_sort":"Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adults"}],"person":[{"role":"aut","display":"Tang, Wannan","roleDisplay":"VerfasserIn","given":"Wannan","family":"Tang"},{"given":"Uwe","family":"Zillmann","role":"aut","roleDisplay":"VerfasserIn","display":"Zillmann, Uwe"},{"family":"Sprengel","given":"Rolf","roleDisplay":"VerfasserIn","display":"Sprengel, Rolf","role":"aut"}],"note":["Gesehen am 28.09.2020"],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"recId":"1733865454","origin":[{"dateIssuedDisp":"14 July 2020","dateIssuedKey":"2020"}],"id":{"eki":["1733865454"],"doi":["10.3389/fnbeh.2020.00115"]},"name":{"displayForm":["Wannan Tang, Uwe Zillmann and Rolf Sprengel"]},"physDesc":[{"extent":"10 S."}],"relHost":[{"part":{"year":"2020","extent":"10","volume":"14","text":"14(2020) Artikel-Nummer 115, 10 Seiten"},"pubHistory":["2007 -"],"recId":"579826392","language":["eng"],"disp":"Alternative anesthesia of neonatal mice for global rAAV delivery in the brain with non-detectable behavioral interference in adultsFrontiers in behavioral neuroscience","note":["Gesehen am 05.10.20"],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title_sort":"Frontiers in behavioral neuroscience","title":"Frontiers in behavioral neuroscience"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"issn":["1662-5153"],"zdb":["2452960-6"],"eki":["579826392"]},"origin":[{"publisherPlace":"Lausanne","dateIssuedKey":"2007","publisher":"Frontiers Research Foundation","dateIssuedDisp":"2007-"}],"name":{"displayForm":["Frontiers Research Foundation"]}}]} | ||
| SRT | |a TANGWANNANALTERNATIV1420 | ||