Mechanoradicals in tensed tendon collagen as a source of oxidative stress
As established nearly a century ago, mechanoradicals originate from homolytic bond scission in polymers. The existence, nature and biological relevance of mechanoradicals in proteins, instead, are unknown. We here show that mechanical stress on collagen produces radicals and subsequently reactive ox...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
08 May 2020
|
| In: |
Nature Communications
Year: 2020, Jahrgang: 11 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-15567-4 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.1038/s41467-020-15567-4 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-020-15567-4 
|
| Verfasserangaben: | Christopher Zapp, Agnieszka Obarska-Kosinska, Benedikt Rennekamp, Markus Kurth, David M. Hudson, Davide Mercadante, Uladzimir Barayeu, Tobias P. Dick, Vasyl Denysenkov, Thomas Prisner, Marina Bennati, Csaba Daday, Reinhard Kappl & Frauke Gräter |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1734491515 | ||
| 003 | DE-627 | ||
| 005 | 20230202152730.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201002s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-020-15567-4 |2 doi | |
| 035 | |a (DE-627)1734491515 | ||
| 035 | |a (DE-599)KXP1734491515 | ||
| 035 | |a (OCoLC)1341368061 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 29 |2 sdnb | ||
| 100 | 1 | |a Zapp, Christopher |e VerfasserIn |0 (DE-588)1218796022 |0 (DE-627)1734492465 |4 aut | |
| 245 | 1 | 0 | |a Mechanoradicals in tensed tendon collagen as a source of oxidative stress |c Christopher Zapp, Agnieszka Obarska-Kosinska, Benedikt Rennekamp, Markus Kurth, David M. Hudson, Davide Mercadante, Uladzimir Barayeu, Tobias P. Dick, Vasyl Denysenkov, Thomas Prisner, Marina Bennati, Csaba Daday, Reinhard Kappl & Frauke Gräter |
| 264 | 1 | |c 08 May 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 02.10.2020 | ||
| 520 | |a As established nearly a century ago, mechanoradicals originate from homolytic bond scission in polymers. The existence, nature and biological relevance of mechanoradicals in proteins, instead, are unknown. We here show that mechanical stress on collagen produces radicals and subsequently reactive oxygen species, essential biological signaling molecules. Electron-paramagnetic resonance (EPR) spectroscopy of stretched rat tail tendon, atomistic molecular dynamics simulations and quantum-chemical calculations show that the radicals form by bond scission in the direct vicinity of crosslinks in collagen. Radicals migrate to adjacent clusters of aromatic residues and stabilize on oxidized tyrosyl radicals, giving rise to a distinct EPR spectrum consistent with a stable dihydroxyphenylalanine (DOPA) radical. The protein mechanoradicals, as a yet undiscovered source of oxidative stress, finally convert into hydrogen peroxide. Our study suggests collagen I to have evolved as a radical sponge against mechano-oxidative damage and proposes a mechanism for exercise-induced oxidative stress and redox-mediated pathophysiological processes. | ||
| 700 | 1 | |a Obarska-Kosinska, Agnieszka |e VerfasserIn |0 (DE-588)1245487108 |0 (DE-627)1777153093 |4 aut | |
| 700 | 1 | |a Rennekamp, Benedikt |d 1994- |e VerfasserIn |0 (DE-588)1197710515 |0 (DE-627)1679443100 |4 aut | |
| 700 | 1 | |a Kurth, Markus |e VerfasserIn |0 (DE-588)1194203760 |0 (DE-627)1676025421 |4 aut | |
| 700 | 1 | |a Hudson, David M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mercadante, Davide |e VerfasserIn |0 (DE-588)1077747241 |0 (DE-627)837433096 |0 (DE-576)446626139 |4 aut | |
| 700 | 1 | |a Barayeu, Uladzimir |d 1993- |e VerfasserIn |0 (DE-588)1278905219 |0 (DE-627)1831805030 |4 aut | |
| 700 | 1 | |a Dick, Tobias P. |e VerfasserIn |0 (DE-588)1142415805 |0 (DE-627)100217807X |0 (DE-576)494813431 |4 aut | |
| 700 | 1 | |a Denysenkov, Vasyl |e VerfasserIn |4 aut | |
| 700 | 1 | |a Prisner, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bennati, Marina |e VerfasserIn |4 aut | |
| 700 | 1 | |a Daday, Csaba |e VerfasserIn |0 (DE-588)1155486730 |0 (DE-627)1017754942 |0 (DE-576)501674284 |4 aut | |
| 700 | 1 | |a Kappl, Reinhard |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gräter, Frauke |e VerfasserIn |0 (DE-588)130664871 |0 (DE-627)505296764 |0 (DE-576)298331314 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature Communications |d [London] : Springer Nature, 2010 |g 11(2020) Artikel-Nummer 2315, 8 Seiten |h Online-Ressource |w (DE-627)626457688 |w (DE-600)2553671-0 |w (DE-576)331555905 |x 2041-1723 |7 nnas |a Mechanoradicals in tensed tendon collagen as a source of oxidative stress |
| 773 | 1 | 8 | |g volume:11 |g year:2020 |a Mechanoradicals in tensed tendon collagen as a source of oxidative stress |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1038/s41467-020-15567-4 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41467-020-15567-4 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201002 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 130664871 |a Gräter, Frauke |m 130664871:Gräter, Frauke |d 700000 |d 708000 |e 700000PG130664871 |e 708000PG130664871 |k 0/700000/ |k 1/700000/708000/ |p 14 |y j | ||
| 998 | |g 1155486730 |a Daday, Csaba |m 1155486730:Daday, Csaba |p 12 | ||
| 998 | |g 1142415805 |a Dick, Tobias P. |m 1142415805:Dick, Tobias P. |d 140000 |e 140000PD1142415805 |k 0/140000/ |p 8 | ||
| 998 | |g 1278905219 |a Barayeu, Uladzimir |m 1278905219:Barayeu, Uladzimir |d 140000 |e 140000PB1278905219 |k 0/140000/ |p 7 | ||
| 998 | |g 1077747241 |a Mercadante, Davide |m 1077747241:Mercadante, Davide |p 6 | ||
| 998 | |g 1194203760 |a Kurth, Markus |m 1194203760:Kurth, Markus |d 700000 |d 708000 |e 700000PK1194203760 |e 708000PK1194203760 |k 0/700000/ |k 1/700000/708000/ |p 4 | ||
| 998 | |g 1197710515 |a Rennekamp, Benedikt |m 1197710515:Rennekamp, Benedikt |d 700000 |d 708000 |e 700000PR1197710515 |e 708000PR1197710515 |k 0/700000/ |k 1/700000/708000/ |p 3 | ||
| 999 | |a KXP-PPN1734491515 |e 3765037583 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"name":{"displayForm":["Christopher Zapp, Agnieszka Obarska-Kosinska, Benedikt Rennekamp, Markus Kurth, David M. Hudson, Davide Mercadante, Uladzimir Barayeu, Tobias P. Dick, Vasyl Denysenkov, Thomas Prisner, Marina Bennati, Csaba Daday, Reinhard Kappl & Frauke Gräter"]},"language":["eng"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1038/s41467-020-15567-4"],"eki":["1734491515"]},"title":[{"title":"Mechanoradicals in tensed tendon collagen as a source of oxidative stress","title_sort":"Mechanoradicals in tensed tendon collagen as a source of oxidative stress"}],"person":[{"family":"Zapp","given":"Christopher","display":"Zapp, Christopher","role":"aut"},{"family":"Obarska-Kosinska","role":"aut","display":"Obarska-Kosinska, Agnieszka","given":"Agnieszka"},{"family":"Rennekamp","display":"Rennekamp, Benedikt","given":"Benedikt","role":"aut"},{"role":"aut","given":"Markus","display":"Kurth, Markus","family":"Kurth"},{"given":"David M.","display":"Hudson, David M.","role":"aut","family":"Hudson"},{"display":"Mercadante, Davide","given":"Davide","role":"aut","family":"Mercadante"},{"family":"Barayeu","role":"aut","given":"Uladzimir","display":"Barayeu, Uladzimir"},{"family":"Dick","given":"Tobias P.","display":"Dick, Tobias P.","role":"aut"},{"family":"Denysenkov","role":"aut","given":"Vasyl","display":"Denysenkov, Vasyl"},{"family":"Prisner","given":"Thomas","display":"Prisner, Thomas","role":"aut"},{"given":"Marina","display":"Bennati, Marina","role":"aut","family":"Bennati"},{"family":"Daday","given":"Csaba","display":"Daday, Csaba","role":"aut"},{"role":"aut","display":"Kappl, Reinhard","given":"Reinhard","family":"Kappl"},{"role":"aut","given":"Frauke","display":"Gräter, Frauke","family":"Gräter"}],"note":["Gesehen am 02.10.2020"],"origin":[{"dateIssuedDisp":"08 May 2020","dateIssuedKey":"2020"}],"relHost":[{"id":{"issn":["2041-1723"],"eki":["626457688"],"zdb":["2553671-0"]},"language":["eng"],"recId":"626457688","origin":[{"publisherPlace":"[London] ; [London]","publisher":"Springer Nature ; Nature Publishing Group UK","dateIssuedDisp":"[2010]-"}],"note":["Gesehen am 13.06.24"],"pubHistory":["2010-"],"title":[{"title_sort":"Nature Communications","title":"Nature Communications"}],"part":{"text":"11(2020) Artikel-Nummer 2315, 8 Seiten","year":"2020","volume":"11"},"disp":"Mechanoradicals in tensed tendon collagen as a source of oxidative stressNature Communications","physDesc":[{"extent":"Online-Ressource"}],"type":{"bibl":"periodical","media":"Online-Ressource"}}],"recId":"1734491515"} | ||
| SRT | |a ZAPPCHRISTMECHANORAD0820 | ||