Adipose-derived stem cells from the breast

Background: - The adipose tissue is deemed as an ideal source of adipose-derived stem cells (ADSCs). Previous studies have reported that ADSCs can be isolated from several organs and locations; however, slight attention has been paid to the breast. We would like to report our experiences in isolatin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Yang, Jie (VerfasserIn) , Xiong, Lingyun (VerfasserIn) , Wang, Rongrong (VerfasserIn) , Sun, Jiaming (VerfasserIn) , Hirche, Christoph (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2014 Feb
In: Journal of research in medical sciences
Year: 2014, Jahrgang: 19, Heft: 2, Pages: 112-116
ISSN:1735-7136
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999595/
Volltext
Verfasserangaben:Jie Yang, Lingyun Xiong, Rongrong Wang, Jiaming Sun, Christoph Hirche

MARC

LEADER 00000caa a2200000 c 4500
001 1734816759
003 DE-627
005 20230427033741.0
007 cr uuu---uuuuu
008 201006s2014 xx |||||o 00| ||eng c
035 |a (DE-627)1734816759 
035 |a (DE-599)KXP1734816759 
035 |a (OCoLC)1341368405 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Yang, Jie  |d 1970-  |e VerfasserIn  |0 (DE-588)1179285646  |0 (DE-627)1066790507  |0 (DE-576)518173453  |4 aut 
245 1 0 |a Adipose-derived stem cells from the breast  |c Jie Yang, Lingyun Xiong, Rongrong Wang, Jiaming Sun, Christoph Hirche 
264 1 |c 2014 Feb 
300 |a 5 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 06.10.2020 
520 |a Background: - The adipose tissue is deemed as an ideal source of adipose-derived stem cells (ADSCs). Previous studies have reported that ADSCs can be isolated from several organs and locations; however, slight attention has been paid to the breast. We would like to report our experiences in isolating breast ADSCs (bADSCs). - - Materials and Methods: - Adipose tissues were harvested from the breasts of seven hypertrophic breast patients. Collagenase I was used to isolate the primary ADSCs. Surface markers were analyzed by flow cytometry. Cellular morphologies were observed. Proliferations of different passages were compared. Viabilities after the cryopreservation were evaluated. Adipogenic and osteogenic differentiation was induced. - - Results: - Primary cultured cells showed morphologies similar to fibroblasts, and expressed surface markers including CD13, CD44, CD90, and CD105. There was no statistical difference of proliferation between different passages (P > 0.05) and between with and without cryopreservation (P > 0.05). Additionally, isolated cells were differentiated into adipocytes and osteoblasts. - - Conclusion: - bADSCs may represent an alternative candidate for tissue engineering. Further studies are needed to obtain more comprehensive understanding on bADSCs. 
700 1 |a Xiong, Lingyun  |d 1986-  |e VerfasserIn  |0 (DE-588)1105079880  |0 (DE-627)862468825  |0 (DE-576)473059517  |4 aut 
700 1 |a Wang, Rongrong  |e VerfasserIn  |4 aut 
700 1 |a Sun, Jiaming  |e VerfasserIn  |4 aut 
700 1 |a Hirche, Christoph  |e VerfasserIn  |0 (DE-588)1074918878  |0 (DE-627)832738484  |0 (DE-576)414117387  |4 aut 
773 0 8 |i Enthalten in  |t Journal of research in medical sciences  |d Isfahan : Farzanegan Rad Andish, 2000  |g 19(2014), 2, Seite 112-116  |h Online-Ressource  |w (DE-627)608492930  |w (DE-600)2513075-4  |w (DE-576)345006291  |x 1735-7136  |7 nnas  |a Adipose-derived stem cells from the breast 
773 1 8 |g volume:19  |g year:2014  |g number:2  |g pages:112-116  |g extent:5  |a Adipose-derived stem cells from the breast 
856 4 0 |u https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999595/  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20201006 
993 |a Article 
994 |a 2014 
998 |g 1074918878  |a Hirche, Christoph  |m 1074918878:Hirche, Christoph  |p 5  |y j 
998 |g 1105079880  |a Xiong, Lingyun  |m 1105079880:Xiong, Lingyun  |p 2 
999 |a KXP-PPN1734816759  |e 3768835804 
BIB |a Y 
SER |a journal 
JSO |a {"recId":"1734816759","language":["eng"],"id":{"eki":["1734816759"]},"physDesc":[{"extent":"5 S."}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Gesehen am 06.10.2020"],"title":[{"title":"Adipose-derived stem cells from the breast","title_sort":"Adipose-derived stem cells from the breast"}],"person":[{"given":"Jie","role":"aut","family":"Yang","display":"Yang, Jie","roleDisplay":"VerfasserIn"},{"role":"aut","given":"Lingyun","family":"Xiong","display":"Xiong, Lingyun","roleDisplay":"VerfasserIn"},{"roleDisplay":"VerfasserIn","family":"Wang","display":"Wang, Rongrong","given":"Rongrong","role":"aut"},{"roleDisplay":"VerfasserIn","family":"Sun","display":"Sun, Jiaming","given":"Jiaming","role":"aut"},{"family":"Hirche","display":"Hirche, Christoph","roleDisplay":"VerfasserIn","given":"Christoph","role":"aut"}],"origin":[{"dateIssuedDisp":"2014 Feb","dateIssuedKey":"2014"}],"name":{"displayForm":["Jie Yang, Lingyun Xiong, Rongrong Wang, Jiaming Sun, Christoph Hirche"]},"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"id":{"eki":["608492930"],"issn":["1735-7136"],"zdb":["2513075-4"]},"titleAlt":[{"title":"JRMS"}],"recId":"608492930","title":[{"title_sort":"Journal of research in medical sciences","title":"Journal of research in medical sciences"}],"note":["Gesehen am 01.10.14"],"type":{"bibl":"periodical","media":"Online-Ressource"},"disp":"Adipose-derived stem cells from the breastJournal of research in medical sciences","pubHistory":["Nachgewiesen 5.2000 -"],"origin":[{"dateIssuedDisp":"2000-","publisher":"Farzanegan Rad Andish","dateIssuedKey":"2000","publisherPlace":"Isfahan"}],"part":{"pages":"112-116","volume":"19","text":"19(2014), 2, Seite 112-116","issue":"2","extent":"5","year":"2014"}}]} 
SRT |a YANGJIEXIOADIPOSEDER2014