The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation
The GTP-bound form of the Ran GTPase (RanGTP), produced around chromosomes, drives nuclear envelope and nuclear pore complex (NPC) re-assembly after mitosis. The nucleoporin MEL-28/ELYS binds chromatin in a RanGTP-regulated manner and acts to seed NPC assembly. Here we show that, upon mitotic NPC di...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
7 Feb 2014
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| In: |
Nature Communications
Year: 2014, Jahrgang: 5 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/ncomms4270 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/ncomms4270 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/ncomms4270 
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| Verfasserangaben: | Hideki Yokoyama, Birgit Koch, Rudolf Walczak, Fulya Ciray-Duygu, Juan Carlos González-Sánchez, Damien P. Devos, Iain W. Mattaj & Oliver J. Gruss |
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| 245 | 1 | 4 | |a The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation |c Hideki Yokoyama, Birgit Koch, Rudolf Walczak, Fulya Ciray-Duygu, Juan Carlos González-Sánchez, Damien P. Devos, Iain W. Mattaj & Oliver J. Gruss |
| 246 | 3 | 3 | |a The nucleoporin MEL-twenty-eight promotes RanGTP-dependent upsilon-tubulin recruitment and microtubule nucleation in mitotic spindle formation |
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| 520 | |a The GTP-bound form of the Ran GTPase (RanGTP), produced around chromosomes, drives nuclear envelope and nuclear pore complex (NPC) re-assembly after mitosis. The nucleoporin MEL-28/ELYS binds chromatin in a RanGTP-regulated manner and acts to seed NPC assembly. Here we show that, upon mitotic NPC disassembly, MEL-28 dissociates from chromatin and re-localizes to spindle microtubules and kinetochores. MEL-28 directly binds microtubules in a RanGTP-regulated way via its C-terminal chromatin-binding domain. Using Xenopus egg extracts, we demonstrate that MEL-28 is essential for RanGTP-dependent microtubule nucleation and spindle assembly, independent of its function in NPC assembly. Specifically, MEL-28 interacts with the γ-tubulin ring complex and recruits it to microtubule nucleation sites. Our data identify MEL-28 as a RanGTP target that functions throughout the cell cycle. Its cell cycle-dependent binding to chromatin or microtubules discriminates MEL-28 functions in interphase and mitosis, and ensures that spindle assembly occurs only after NPC breakdown. | ||
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