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High contrast visualization of cell-hydrogel contact by advanced interferometric optical microscopy

Hydrogels with tunable elasticity has been widely used as micromechanical environment models for cells. However, the imaging of physical contacts between cells and hydrogels with a nanometer resolution along the optical axis remain challenging because of low reflectivity at hydrogel-liquid interface...

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Bibliographische Detailangaben
Hauptverfasser: Matsuzaki, Takahisa (VerfasserIn) , Sazaki, Gen (VerfasserIn) , Suganuma, Masami (VerfasserIn) , Watanabe, Tatsuro (VerfasserIn) , Yamazaki, Takashi (VerfasserIn) , Tanaka, Motomu (VerfasserIn) , Nakabayashi, Seiichiro (VerfasserIn) , Yoshikawa, Hiroshi Y. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2014
In: The journal of physical chemistry letters
Year: 2013, Jahrgang: 5, Heft: 1, Pages: 253-257
ISSN:1948-7185
DOI:10.1021/jz402463u
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/jz402463u
Verlag, lizenzpflichtig, Volltext: https://pubs.acs.org/doi/10.1021/jz402463u
Volltext
Verfasserangaben:Takahisa Matsuzaki, Gen Sazaki, Masami Suganuma, Tatsuro Watanabe, Takashi Yamazaki, Motomu Tanaka, Seiichiro Nakabayashi, and Hiroshi Y. Yoshikawa
Beschreibung
Zusammenfassung:Hydrogels with tunable elasticity has been widely used as micromechanical environment models for cells. However, the imaging of physical contacts between cells and hydrogels with a nanometer resolution along the optical axis remain challenging because of low reflectivity at hydrogel-liquid interface. In this work, we have developed an advanced interferometric optical microscopy for the high contrast visualization of cell-hydrogel contact. Here, reflection interference contrast microscopy (RICM) was modified with a confocal unit, high throughput optics and coherent monochromatic light sources to enhance interferometric signals from cell-hydrogel contact zones. The advanced interferomety clearly visualized physical contacts between cells and hydrogels, and thus enabled the quantitative evaluation of the area of cell-hydrogel adhesion.
Beschreibung:Published: December 19, 2013
Gesehen am 13.10.2020
Beschreibung:Online Resource
ISSN:1948-7185
DOI:10.1021/jz402463u

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