Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets
Essentials Human salivary extracellular vesicles (EVs) expose coagulant tissue factor (TF). Salivary EVs expose CD24, a ligand of P-selectin. CD24 and coagulant TF co-localize on salivary EVs. TF+/CD24+ salivary EVs bind to activated platelets and trigger coagulation. Summary Background Extracellula...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
15 April 2018
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| In: |
Journal of thrombosis and haemostasis
Year: 2018, Volume: 16, Issue: 6, Pages: 1153-1163 |
| ISSN: | 1538-7836 |
| DOI: | 10.1111/jth.14023 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/jth.14023 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/jth.14023 
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| Author Notes: | Y. Yu, E. Gool, R.J. Berckmans, F.a.W. Coumans, A.D. Barendrecht, C. Maas, N.N. van der Wel, P. Altevogt, A. Sturk, R. Nieuwland |
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| 245 | 1 | 0 | |a Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets |c Y. Yu, E. Gool, R.J. Berckmans, F.a.W. Coumans, A.D. Barendrecht, C. Maas, N.N. van der Wel, P. Altevogt, A. Sturk, R. Nieuwland |
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| 520 | |a Essentials Human salivary extracellular vesicles (EVs) expose coagulant tissue factor (TF). Salivary EVs expose CD24, a ligand of P-selectin. CD24 and coagulant TF co-localize on salivary EVs. TF+/CD24+ salivary EVs bind to activated platelets and trigger coagulation. Summary Background Extracellular vesicles (EVs) from human saliva expose coagulant tissue factor (TF). Whether such TF-exposing EVs contribute to hemostasis, however, is unknown. Recently, in a mice model, tumor cell-derived EVs were shown to deliver coagulant TF to activated platelets at a site of vascular injury via interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin. Objectives We hypothesized that salivary EVs may deliver coagulant TF to activated platelets via interaction with P-selectin. Methods We investigated the presence of two ligands of P-selectin on salivary EVs, PSGL-1 and CD24. Results Salivary EVs expose CD24 but PSGL-1 was not detected. Immune depletion of CD24-exposing EVs completely abolished the TF-dependent coagulant activity of cell-free saliva, showing that coagulant TF and CD24 co-localize on salivary EVs. In a whole blood perfusion model, salivary EVs accumulated at the surface of activated platelets and promoted fibrin generation, which was abolished by an inhibitory antibody against human CD24. Conclusions A subset of EVs in human saliva expose coagulant TF and CD24, a ligand of P-selectin, suggesting that such EVs may facilitate hemostasis at a site of skin injury where the wound is licked in a reflex action. | ||
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