F2RL3 methylation as a biomarker of current and lifetime smoking exposures
Background: Recent genome-wide DNA methylation studies have found a pronounced difference in methylation of the F2RL3 gene (also known as PAR-4) in blood DNA according to smoking exposure. Knowledge on the variation of F2RL3 methylation by various degrees of smoking exposure is still very sparse.Obj...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1 February 2014
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| In: |
Environmental health perspectives
Year: 2014, Volume: 122, Issue: 2, Pages: 131-137 |
| ISSN: | 1552-9924 |
| DOI: | 10.1289/ehp.1306937 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1289/ehp.1306937 Verlag, lizenzpflichtig, Volltext: https://ehp.niehs.nih.gov/doi/10.1289/ehp.1306937 
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| Author Notes: | Yan Zhang, Rongxi Yang, Barbara Burwinkel, Lutz P. Breitling, and Hermann Brenner |
| Summary: | Background: Recent genome-wide DNA methylation studies have found a pronounced difference in methylation of the F2RL3 gene (also known as PAR-4) in blood DNA according to smoking exposure. Knowledge on the variation of F2RL3 methylation by various degrees of smoking exposure is still very sparse.Objectives: We aimed to assess dose-response relationships of current and lifetime active smoking exposure with F2RL3 methylation.Methods: In a large population-based study, we quantified blood DNA methylation at F2RL3 for 3,588 participants using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Associations of smoking exposure with methylation intensity were examined by multiple linear regression, controlling for potential confounding factors and paying particular attention to dose-response patterns with respect to current and lifetime smoking exposure as well as time since cessation of smoking.Results: F2RL3 methylation intensity showed a strong association with smoking status (p < 0.0001), which persisted after controlling for potential confounding factors. Clear inverse dose-response relationships with F2RL3 methylation intensity were seen for both current intensity and lifetime pack-years of smoking. Among former smokers, F2RL3 methylation intensity increased gradually from levels close to those of current smokers for recent quitters to levels close to never smokers for long-term (> 20 years) quitters.Conclusions: F2RL3 methylation is a promising biomarker for both current and long-term past tobacco exposure, and its predictive value for smoking-related diseases warrants further exploration.Citation: Zhang Y, Yang R, Burwinkel B, Breitling LP, Brenner H. 2014. F2RL3 methylation as a biomarker of current and lifetime smoking exposures. Environ Health Perspect 122:131-137;  http://dx.doi.org/10.1289/ehp.1306937 |
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| Item Description: | Gesehen am 21.10.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1552-9924 |
| DOI: | 10.1289/ehp.1306937 |