OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease
The hepatitis C virus (HCV) nonstructural protein 3-4A (NS3-4A) protease is a key component of the viral replication complex and the target of protease inhibitors used in current clinical practice. By cleaving and thereby inactivating selected host factors it also plays a role in the persistence and...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 22, 2020
|
| In: |
PLOS ONE
Year: 2020, Jahrgang: 15, Heft: 7 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0236447 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1371/journal.pone.0236447 Verlag, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236447 
|
| Verfasserangaben: | Huong T. L. Tran, Kenichi Morikawa, Anggakusuma, Rose Zibi, Viet Loan Dao Thi, François Penin, Markus H. Heim, Manfredo Quadroni, Thomas Pietschmann, Jérôme Gouttenoire, Darius Moradpour |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1736587137 | ||
| 003 | DE-627 | ||
| 005 | 20220819001302.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201026s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1371/journal.pone.0236447 |2 doi | |
| 035 | |a (DE-627)1736587137 | ||
| 035 | |a (DE-599)KXP1736587137 | ||
| 035 | |a (OCoLC)1341373956 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Tran, Huong T. L. |e VerfasserIn |0 (DE-588)1220247561 |0 (DE-627)1736586661 |4 aut | |
| 245 | 1 | 0 | |a OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease |c Huong T. L. Tran, Kenichi Morikawa, Anggakusuma, Rose Zibi, Viet Loan Dao Thi, François Penin, Markus H. Heim, Manfredo Quadroni, Thomas Pietschmann, Jérôme Gouttenoire, Darius Moradpour |
| 264 | 1 | |c July 22, 2020 | |
| 300 | |a 18 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 26.10.2020 | ||
| 520 | |a The hepatitis C virus (HCV) nonstructural protein 3-4A (NS3-4A) protease is a key component of the viral replication complex and the target of protease inhibitors used in current clinical practice. By cleaving and thereby inactivating selected host factors it also plays a role in the persistence and pathogenesis of hepatitis C. Here, we describe ovarian cancer immunoreactive antigen domain containing protein 1 (OCIAD1) as a novel cellular substrate of the HCV NS3-4A protease. OCIAD1 was identified by quantitative proteomics involving stable isotopic labeling using amino acids in cell culture coupled with mass spectrometry. It is a poorly characterized membrane protein believed to be involved in cancer development. OCIAD1 is cleaved by the NS3-4A protease at Cys 38, close to a predicted transmembrane segment. Cleavage was observed in heterologous expression systems, the replicon and cell culture-derived HCV systems, as well as in liver biopsies from patients with chronic hepatitis C. NS3-4A proteases from diverse hepacivirus species efficiently cleaved OCIAD1. The subcellular localization of OCIAD1 on mitochondria was not altered by NS3-4A-mediated cleavage. Interestingly, OCIAD2, a homolog of OCIAD1 with a cysteine residue in a similar position and identical subcellular localization, was not cleaved by NS3-4A. Domain swapping experiments revealed that the sequence surrounding the cleavage site as well as the predicted transmembrane segment contribute to substrate selectivity. Overexpression as well as knock down and rescue experiments did not affect the HCV life cycle in vitro, raising the possibility that OCIAD1 may be involved in the pathogenesis of hepatitis C in vivo. | ||
| 700 | 1 | |a Dao Thi, Viet Loan |e VerfasserIn |0 (DE-588)1186079657 |0 (DE-627)1665348402 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PLOS ONE |d San Francisco, California, US : PLOS, 2006 |g 15(2020,7) Artikel-Nummer e0236447, 18 Seiten |h Online-Ressource |w (DE-627)523574592 |w (DE-600)2267670-3 |w (DE-576)281331979 |x 1932-6203 |7 nnas |a OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease |
| 773 | 1 | 8 | |g volume:15 |g year:2020 |g number:7 |g extent:18 |a OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease |
| 856 | 4 | 0 | |u https://doi.org/10.1371/journal.pone.0236447 |x Verlag |x Resolving-System |3 Volltext |
| 856 | 4 | 0 | |u https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0236447 |x Verlag |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201026 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1186079657 |a Dao Thi, Viet Loan |m 1186079657:Dao Thi, Viet Loan |d 910000 |d 911700 |e 910000PD1186079657 |e 911700PD1186079657 |k 0/910000/ |k 1/910000/911700/ |p 5 | ||
| 999 | |a KXP-PPN1736587137 |e 378640612X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1736587137","note":["Gesehen am 26.10.2020"],"name":{"displayForm":["Huong T. L. Tran, Kenichi Morikawa, Anggakusuma, Rose Zibi, Viet Loan Dao Thi, François Penin, Markus H. Heim, Manfredo Quadroni, Thomas Pietschmann, Jérôme Gouttenoire, Darius Moradpour"]},"relHost":[{"disp":"OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A proteasePLOS ONE","name":{"displayForm":["Public Library of Science"]},"pubHistory":["1.2006 -"],"note":["Schreibweise des Titels bis 2012: PLoS ONE","Gesehen am 20.03.19"],"recId":"523574592","origin":[{"dateIssuedDisp":"2006-","publisherPlace":"San Francisco, California, US ; Lawrence, Kan.","publisher":"PLOS ; PLoS","dateIssuedKey":"2006"}],"title":[{"title":"PLOS ONE","title_sort":"PLOS ONE"}],"physDesc":[{"extent":"Online-Ressource"}],"part":{"text":"15(2020,7) Artikel-Nummer e0236447, 18 Seiten","issue":"7","extent":"18","year":"2020","volume":"15"},"id":{"eki":["523574592"],"issn":["1932-6203"],"zdb":["2267670-3"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"corporate":[{"display":"Public Library of Science","role":"isb"}]}],"person":[{"given":"Huong T. L.","display":"Tran, Huong T. L.","family":"Tran","role":"aut"},{"role":"aut","family":"Dao Thi","display":"Dao Thi, Viet Loan","given":"Viet Loan"}],"title":[{"title_sort":"OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease","title":"OCIAD1 is a host mitochondrial substrate of the hepatitis C virus NS3-4A protease"}],"physDesc":[{"extent":"18 S."}],"origin":[{"dateIssuedDisp":"July 22, 2020","dateIssuedKey":"2020"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"doi":["10.1371/journal.pone.0236447"],"eki":["1736587137"]},"language":["eng"]} | ||
| SRT | |a TRANHUONGTOCIAD1ISAH2220 | ||