Stem cell-derived polarized hepatocytes
Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell differentiation protocol that uses transwell filters to genera...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
03 April 2020
|
| In: |
Nature Communications
Year: 2020, Volume: 11 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-15337-2 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-020-15337-2 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-020-15337-2 
|
| Author Notes: | Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon & Charles M. Rice |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1737357542 | ||
| 003 | DE-627 | ||
| 005 | 20240407193414.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201029s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41467-020-15337-2 |2 doi | |
| 035 | |a (DE-627)1737357542 | ||
| 035 | |a (DE-599)KXP1737357542 | ||
| 035 | |a (OCoLC)1341375219 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 32 |2 sdnb | ||
| 100 | 1 | |a Dao Thi, Viet Loan |e VerfasserIn |0 (DE-588)1186079657 |0 (DE-627)1665348402 |4 aut | |
| 245 | 1 | 0 | |a Stem cell-derived polarized hepatocytes |c Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon & Charles M. Rice |
| 264 | 1 | |c 03 April 2020 | |
| 300 | |a 13 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 29.10.2020 | ||
| 520 | |a Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver biology. Despite their numerous advantages, HLCs lack critical in vivo characteristics, including cell polarity. Here, we report a stem cell differentiation protocol that uses transwell filters to generate columnar polarized HLCs with clearly defined basolateral and apical membranes separated by tight junctions. We show that polarized HLCs secrete cargo directionally: Albumin, urea, and lipoproteins are secreted basolaterally, whereas bile acids are secreted apically. Further, we show that enterically transmitted hepatitis E virus (HEV) progeny particles are secreted basolaterally as quasi-enveloped particles and apically as naked virions, recapitulating essential steps of the natural infectious cycle in vivo. We also provide proof-of-concept that polarized HLCs can be used for pharmacokinetic and drug-drug interaction studies. This novel system provides a powerful tool to study hepatocyte biology, disease mechanisms, genetic variation, and drug metabolism in a more physiologically relevant setting. | ||
| 700 | 1 | |a Wu, Xianfang |e VerfasserIn |4 aut | |
| 700 | 1 | |a Belote, Rachel L. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Andreo, Ursula |e VerfasserIn |4 aut | |
| 700 | 1 | |a Takacs, Constantin N. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Fernandez, Joseph P. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Vale Silva, Luis A. |e VerfasserIn |0 (DE-588)1179957113 |0 (DE-627)1067566864 |0 (DE-576)51842054X |4 aut | |
| 700 | 1 | |a Prallet, Sarah |e VerfasserIn |4 aut | |
| 700 | 1 | |a Decker, Charlotte Caroline |e VerfasserIn |0 (DE-588)1180439538 |0 (DE-627)1067723625 |0 (DE-576)520173600 |4 aut | |
| 700 | 1 | |a Fu, Rebecca Menhua |d 1993- |e VerfasserIn |0 (DE-588)1214598528 |0 (DE-627)1725566583 |4 aut | |
| 700 | 1 | |a Qu, Bingqian |d 1984- |e VerfasserIn |0 (DE-588)1164252194 |0 (DE-627)1028805802 |0 (DE-576)508467160 |4 aut | |
| 700 | 1 | |a Uryu, Kunihiro |e VerfasserIn |4 aut | |
| 700 | 1 | |a Molina, Henrik |e VerfasserIn |4 aut | |
| 700 | 1 | |a Saeed, Mohsan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Steinmann, Eike |e VerfasserIn |4 aut | |
| 700 | 1 | |a Urban, Stephan |e VerfasserIn |0 (DE-588)1060263599 |0 (DE-627)79935399X |0 (DE-576)174162049 |4 aut | |
| 700 | 1 | |a Singaraja, Roshni R. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schneider, William M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Simon, Sanford M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Rice, Charles M. |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature Communications |d [London] : Springer Nature, 2010 |g 11(2020) Artikel-Nummer 1677, 13 Seiten |h Online-Ressource |w (DE-627)626457688 |w (DE-600)2553671-0 |w (DE-576)331555905 |x 2041-1723 |7 nnas |a Stem cell-derived polarized hepatocytes |
| 773 | 1 | 8 | |g volume:11 |g year:2020 |g extent:13 |a Stem cell-derived polarized hepatocytes |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-020-15337-2 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.nature.com/articles/s41467-020-15337-2 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201029 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1060263599 |a Urban, Stephan |m 1060263599:Urban, Stephan |d 910000 |d 911700 |e 910000PU1060263599 |e 911700PU1060263599 |k 0/910000/ |k 1/910000/911700/ |p 16 | ||
| 998 | |g 1164252194 |a Qu, Bingqian |m 1164252194:Qu, Bingqian |d 910000 |d 911700 |e 910000PQ1164252194 |e 911700PQ1164252194 |k 0/910000/ |k 1/910000/911700/ |p 11 | ||
| 998 | |g 1214598528 |a Fu, Rebecca Menhua |m 1214598528:Fu, Rebecca Menhua |d 910000 |d 911700 |e 910000PF1214598528 |e 911700PF1214598528 |k 0/910000/ |k 1/910000/911700/ |p 10 | ||
| 998 | |g 1180439538 |a Decker, Charlotte Caroline |m 1180439538:Decker, Charlotte Caroline |d 910000 |d 911700 |e 910000PD1180439538 |e 911700PD1180439538 |k 0/910000/ |k 1/910000/911700/ |p 9 | ||
| 998 | |g 1179957113 |a Vale Silva, Luis A. |m 1179957113:Vale Silva, Luis A. |d 160000 |d 160100 |d 700000 |d 716000 |e 160000PV1179957113 |e 160100PV1179957113 |e 700000PV1179957113 |e 716000PV1179957113 |k 0/160000/ |k 1/160000/160100/ |k 0/700000/ |k 1/700000/716000/ |p 7 | ||
| 998 | |g 1186079657 |a Dao Thi, Viet Loan |m 1186079657:Dao Thi, Viet Loan |d 910000 |d 911700 |e 910000PD1186079657 |e 911700PD1186079657 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j | ||
| 999 | |a KXP-PPN1737357542 |e 3789110671 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"type":{"media":"Online-Ressource","bibl":"article-journal"},"id":{"eki":["1737357542"],"doi":["10.1038/s41467-020-15337-2"]},"language":["eng"],"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"03 April 2020"}],"person":[{"role":"aut","display":"Dao Thi, Viet Loan","family":"Dao Thi","given":"Viet Loan"},{"role":"aut","given":"Xianfang","family":"Wu","display":"Wu, Xianfang"},{"role":"aut","given":"Rachel L.","family":"Belote","display":"Belote, Rachel L."},{"given":"Ursula","display":"Andreo, Ursula","family":"Andreo","role":"aut"},{"role":"aut","given":"Constantin N.","family":"Takacs","display":"Takacs, Constantin N."},{"role":"aut","given":"Joseph P.","family":"Fernandez","display":"Fernandez, Joseph P."},{"display":"Vale Silva, Luis A.","family":"Vale Silva","given":"Luis A.","role":"aut"},{"role":"aut","family":"Prallet","display":"Prallet, Sarah","given":"Sarah"},{"given":"Charlotte Caroline","family":"Decker","display":"Decker, Charlotte Caroline","role":"aut"},{"role":"aut","family":"Fu","display":"Fu, Rebecca Menhua","given":"Rebecca Menhua"},{"given":"Bingqian","display":"Qu, Bingqian","family":"Qu","role":"aut"},{"role":"aut","given":"Kunihiro","display":"Uryu, Kunihiro","family":"Uryu"},{"display":"Molina, Henrik","family":"Molina","given":"Henrik","role":"aut"},{"given":"Mohsan","family":"Saeed","display":"Saeed, Mohsan","role":"aut"},{"display":"Steinmann, Eike","family":"Steinmann","given":"Eike","role":"aut"},{"family":"Urban","display":"Urban, Stephan","given":"Stephan","role":"aut"},{"role":"aut","display":"Singaraja, Roshni R.","family":"Singaraja","given":"Roshni R."},{"role":"aut","given":"William M.","display":"Schneider, William M.","family":"Schneider"},{"family":"Simon","display":"Simon, Sanford M.","given":"Sanford M.","role":"aut"},{"role":"aut","given":"Charles M.","display":"Rice, Charles M.","family":"Rice"}],"title":[{"title":"Stem cell-derived polarized hepatocytes","title_sort":"Stem cell-derived polarized hepatocytes"}],"physDesc":[{"extent":"13 S."}],"relHost":[{"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"Nature Communications","title_sort":"Nature Communications"}],"part":{"text":"11(2020) Artikel-Nummer 1677, 13 Seiten","volume":"11","extent":"13","year":"2020"},"origin":[{"dateIssuedDisp":"[2010]-","publisherPlace":"[London] ; [London]","publisher":"Springer Nature ; Nature Publishing Group UK"}],"id":{"issn":["2041-1723"],"zdb":["2553671-0"],"eki":["626457688"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"language":["eng"],"pubHistory":["2010-"],"note":["Gesehen am 13.06.24"],"recId":"626457688","disp":"Stem cell-derived polarized hepatocytesNature Communications"}],"name":{"displayForm":["Viet Loan Dao Thi, Xianfang Wu, Rachel L. Belote, Ursula Andreo, Constantin N. Takacs, Joseph P. Fernandez, Luis Andre Vale-Silva, Sarah Prallet, Charlotte C. Decker, Rebecca M. Fu, Bingqian Qu, Kunihiro Uryu, Henrik Molina, Mohsan Saeed, Eike Steinmann, Stephan Urban, Roshni R. Singaraja, William M. Schneider, Sanford M. Simon & Charles M. Rice"]},"recId":"1737357542","note":["Gesehen am 29.10.2020"]} | ||
| SRT | |a DAOTHIVIETSTEMCELLDE0320 | ||