Droplet-based cytotoxicity assay: implementation of time-efficient screening of antitumor activity of natural killer cells
Natural killer (NK) cells are key players of the innate immune system. Due to their rapid cytotoxicity against infectious pathogens, hematologic malignancies, and solid tumors, NK cells represent solid candidates for cell-based immunotherapy. Despite the progress made in recent years, the heterogene...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
September 17, 2020
|
| In: |
ACS omega
Year: 2020, Jahrgang: 5, Heft: 38, Pages: 24674-24683 |
| ISSN: | 2470-1343 |
| DOI: | 10.1021/acsomega.0c03264 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1021/acsomega.0c03264
|
| Verfasserangaben: | Silvia Antona, Ilia Platzman, and Joachim P. Spatz |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1737995174 | ||
| 003 | DE-627 | ||
| 005 | 20230426130453.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201105s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acsomega.0c03264 |2 doi | |
| 035 | |a (DE-627)1737995174 | ||
| 035 | |a (DE-599)KXP1737995174 | ||
| 035 | |a (OCoLC)1341375553 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Antona, Silvia |d 1991- |e VerfasserIn |0 (DE-588)1192701550 |0 (DE-627)167119845X |4 aut | |
| 245 | 1 | 0 | |a Droplet-based cytotoxicity assay |b implementation of time-efficient screening of antitumor activity of natural killer cells |c Silvia Antona, Ilia Platzman, and Joachim P. Spatz |
| 264 | 1 | |c September 17, 2020 | |
| 300 | |a 10 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 05.11.2020 | ||
| 520 | |a Natural killer (NK) cells are key players of the innate immune system. Due to their rapid cytotoxicity against infectious pathogens, hematologic malignancies, and solid tumors, NK cells represent solid candidates for cell-based immunotherapy. Despite the progress made in recent years, the heterogeneity in their cytotoxic behavior represents a drawback. With the goal of screening the intrinsic diversity of NK cells, droplet-based microfluidic technology is exploited to develop a single-cell time-efficient cytotoxicity assay. Toward this end, NK-92 cells are coencapsulated with hematological tumor cell lines in water-in-oil droplets of different sizes and their cytotoxic activity is evaluated. The effect of droplet-based confinement on NK cytotoxicity is investigated by controlling the droplet volume. The successful optimization of the droplet size allows for time efficiency compared to cytotoxicity assays based on flow cytometry. Additionally, the ability of individual NK-92 cells to kill multiple target cells in series is explored, expanding the knowledge about the serial killing process dynamics. The developed droplet-based microfluidic assay does not require the labeling of NK cells and represents a step toward developing of a forthcoming process for the selection of NK cells with the highest cytotoxicity against specific targets. | ||
| 700 | 1 | |a Platzman, Ilia |e VerfasserIn |0 (DE-588)1166793680 |0 (DE-627)1030663440 |0 (DE-576)510898327 |4 aut | |
| 700 | 1 | |a Spatz, Joachim P. |d 1969- |e VerfasserIn |0 (DE-588)122582675 |0 (DE-627)08203124X |0 (DE-576)173014771 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t ACS omega |d Washington, DC : ACS Publications, 2016 |g 5(2020), 38, Seite 24674-24683 |h Online-Ressource |w (DE-627)863132308 |w (DE-600)2861993-6 |w (DE-576)474097676 |x 2470-1343 |7 nnas |a Droplet-based cytotoxicity assay implementation of time-efficient screening of antitumor activity of natural killer cells |
| 773 | 1 | 8 | |g volume:5 |g year:2020 |g number:38 |g pages:24674-24683 |g extent:10 |a Droplet-based cytotoxicity assay implementation of time-efficient screening of antitumor activity of natural killer cells |
| 856 | 4 | 0 | |u https://doi.org/10.1021/acsomega.0c03264 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201105 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 122582675 |a Spatz, Joachim P. |m 122582675:Spatz, Joachim P. |d 120000 |d 120300 |e 120000PS122582675 |e 120300PS122582675 |k 0/120000/ |k 1/120000/120300/ |p 3 |y j | ||
| 998 | |g 1166793680 |a Platzman, Ilia |m 1166793680:Platzman, Ilia |p 2 | ||
| 998 | |g 1192701550 |a Antona, Silvia |m 1192701550:Antona, Silvia |d 50000 |e 50000PA1192701550 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1737995174 |e 3792702428 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"physDesc":[{"extent":"10 S."}],"relHost":[{"part":{"pages":"24674-24683","issue":"38","year":"2020","extent":"10","text":"5(2020), 38, Seite 24674-24683","volume":"5"},"pubHistory":["Volume 1, issue 1 (July 2016)-"],"recId":"863132308","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"American Chemical Society","role":"isb"}],"note":["Gesehen am 25.06.2025"],"disp":"Droplet-based cytotoxicity assay implementation of time-efficient screening of antitumor activity of natural killer cellsACS omega","type":{"media":"Online-Ressource","bibl":"periodical"},"title":[{"title_sort":"ACS omega","title":"ACS omega"}],"physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["2861993-6"],"eki":["863132308"],"issn":["2470-1343"]},"origin":[{"publisherPlace":"Washington, DC","dateIssuedDisp":"[2016]-","publisher":"ACS Publications"}],"name":{"displayForm":["American Chemical Society"]}}],"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"September 17, 2020"}],"id":{"doi":["10.1021/acsomega.0c03264"],"eki":["1737995174"]},"name":{"displayForm":["Silvia Antona, Ilia Platzman, and Joachim P. Spatz"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 05.11.2020"],"language":["eng"],"recId":"1737995174","title":[{"subtitle":"implementation of time-efficient screening of antitumor activity of natural killer cells","title":"Droplet-based cytotoxicity assay","title_sort":"Droplet-based cytotoxicity assay"}],"person":[{"display":"Antona, Silvia","roleDisplay":"VerfasserIn","role":"aut","family":"Antona","given":"Silvia"},{"given":"Ilia","family":"Platzman","role":"aut","roleDisplay":"VerfasserIn","display":"Platzman, Ilia"},{"roleDisplay":"VerfasserIn","display":"Spatz, Joachim P.","role":"aut","family":"Spatz","given":"Joachim P."}]} | ||
| SRT | |a ANTONASILVDROPLETBAS1720 | ||