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Detection of severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) by mass spectrometry

Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have bee...

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Hauptverfasser: Wandernoth, Petra Melitta (VerfasserIn) , Kriegsmann, Katharina (VerfasserIn) , Groh-Mohanu, Cristina (VerfasserIn) , Daeumer, Martin (VerfasserIn) , Gohl, Peter (VerfasserIn) , Harzer, Oliver (VerfasserIn) , Kriegsmann, Mark (VerfasserIn) , Kriegsmann, Joerg (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 4 August 2020
In: Viruses
Year: 2020, Jahrgang: 12, Heft: 8
ISSN:1999-4915
DOI:10.3390/v12080849
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/v12080849
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1999-4915/12/8/849
Volltext
Verfasserangaben:Petra Wandernoth, Katharina Kriegsmann, Cristina Groh-Mohanu, Martin Daeumer, Peter Gohl, Oliver Harzer, Mark Kriegsmann and Joerg Kriegsmann
Beschreibung
Zusammenfassung:Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS). Methods: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared. Results: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays. Conclusions: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
Beschreibung:Gesehen am 09.11.2020
Beschreibung:Online Resource
ISSN:1999-4915
DOI:10.3390/v12080849

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