Alu element-containing RNAs maintain nucleolar structure and function
Abstract Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
13 October 2015
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| In: |
The EMBO journal
Year: 2015, Jahrgang: 34, Heft: 22, Pages: 2758-2774 |
| ISSN: | 1460-2075 |
| DOI: | 10.15252/embj.201591458 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.15252/embj.201591458 Verlag, lizenzpflichtig, Volltext: https://www.embopress.org/doi/full/10.15252/embj.201591458 
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| Verfasserangaben: | Maïwen Caudron-Herger, Teresa Pankert, Jeanette Seiler, Attila Németh, Renate Voit, Ingrid Grummt & Karsten Rippe |
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| 520 | |a Abstract Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense-oligo-mediated depletion of aluRNAs or drug-induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I-dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre-rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre-rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA-based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production. | ||
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