Cover Image

Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures

Background: Exposure of the developing brain to ethanol disposes the fetus to fetal alcohol syndrome and causes a number of changes in several neurochemical systems. In particular, the glutamatergic system is affected by ethanol. Thus, increased sensitivity of glutamate receptors and enhanced transm...

Full description

Saved in:
Bibliographic Details
Main Authors: Zink, Mathias (Author) , Schmitt, Andrea (Author) , Vengeliene, Valentina (Author) , Henn, Fritz A. (Author) , Spanagel, Rainer (Author)
Format: Article (Journal)
Language:English
Published: 2004
In: Alcoholism
Year: 2004, Volume: 28, Issue: 11, Pages: 1752-1757
ISSN:1530-0277
DOI:https://doi.org/10.1097/01.ALC.0000145810.12545.B3
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1097/01.ALC.0000145810.12545.B3
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1097/01.ALC.0000145810.12545.B3
Get full text
Author Notes:Mathias Zink, Andrea Schmitt, Valentina Vengeliene, Fritz A. Henn, Rainer Spanagel

MARC

LEADER 00000caa a2200000 c 4500
001 1738417360
003 DE-627
005 20220819020850.0
007 cr uuu---uuuuu
008 201111s2004 xx |||||o 00| ||eng c
024 7 |a 10.1097/01.ALC.0000145810.12545.B3  |2 doi 
035 |a (DE-627)1738417360 
035 |a (DE-599)KXP1738417360 
035 |a (OCoLC)1341376410 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Zink, Mathias  |d 1968-  |e VerfasserIn  |0 (DE-588)121715221  |0 (DE-627)705627217  |0 (DE-576)292845715  |4 aut 
245 1 0 |a Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures  |c Mathias Zink, Andrea Schmitt, Valentina Vengeliene, Fritz A. Henn, Rainer Spanagel 
264 1 |c 2004 
300 |a 6 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 11.11.2020 
520 |a Background: Exposure of the developing brain to ethanol disposes the fetus to fetal alcohol syndrome and causes a number of changes in several neurochemical systems. In particular, the glutamatergic system is affected by ethanol. Thus, increased sensitivity of glutamate receptors and enhanced transmembrane transport of glutamate were found in primary astrocyte cultures. However, in these in vitro studies, changes in the expression of glutamate transporters were not detected. To further characterize the influence of chronic ethanol exposure on the developing brain, we assessed the transcriptional and translational regulation of glutamate transporters in a less artificial in vitro system. Methods: We exposed postnatal rat cortical organotypic slice cultures to ethanol (100 mM) for 4 and 8 days. Expression of the excitatory amino acid transporters EAAT1 and EAAT2 was analyzed in comparison to untreated controls with semiquantitative reverse transcriptase-polymerase chain reaction. In addition, changes in protein expression were detected by Western blotting. Results: In ethanol-exposed cortical slices, we observed more prominent fiber outgrowth and significantly increased gene expression (EAAT1: +35%, p= 0.029; EAAT2: +251%, p= 0.015). These findings could be verified on the protein level, because Western blot analysis revealed significantly higher levels of EAAT1 (+76%; p= 0.008) and EAAT2 (+104%; p= 0.018) in ethanol-treated slices compared with controls. Conclusions: Our results are in concert with earlier studies describing the induction of glutamate transport by ethanol. Enhanced expression of EAAT1 and EAAT2 after ethanol exposure can be viewed as a maladaptive process that disposes the developing brain to fetal alcohol syndrome. 
650 4 |a Development 
650 4 |a EAAT 
650 4 |a Ethanol 
650 4 |a Fetal Alcohol Syndrome 
650 4 |a Glutamate 
700 1 |a Schmitt, Andrea  |e VerfasserIn  |0 (DE-588)1045120928  |0 (DE-627)773824014  |0 (DE-576)39876655X  |4 aut 
700 1 |a Vengeliene, Valentina  |e VerfasserIn  |0 (DE-588)130680516  |0 (DE-627)505582236  |0 (DE-576)298340542  |4 aut 
700 1 |a Henn, Fritz A.  |d 1941-  |e VerfasserIn  |0 (DE-588)17213384X  |0 (DE-627)697047938  |0 (DE-576)133005917  |4 aut 
700 1 |a Spanagel, Rainer  |d 1961-  |e VerfasserIn  |0 (DE-588)112706312  |0 (DE-627)521941261  |0 (DE-576)289740002  |4 aut 
773 0 8 |i Enthalten in  |t Alcoholism  |d Oxford [u.a.] : Wiley-Blackwell, 1977  |g 28(2004), 11, Seite 1752-1757  |h Online-Ressource  |w (DE-627)329270001  |w (DE-600)2046886-6  |w (DE-576)094502307  |x 1530-0277  |7 nnas  |a Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures 
773 1 8 |g volume:28  |g year:2004  |g number:11  |g pages:1752-1757  |g extent:6  |a Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures 
856 4 0 |u https://doi.org/https://doi.org/10.1097/01.ALC.0000145810.12545.B3  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://onlinelibrary.wiley.com/doi/abs/10.1097/01.ALC.0000145810.12545.B3  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20201111 
993 |a Article 
994 |a 2004 
998 |g 112706312  |a Spanagel, Rainer  |m 112706312:Spanagel, Rainer  |d 60000  |e 60000PS112706312  |k 0/60000/  |p 5  |y j 
998 |g 17213384X  |a Henn, Fritz A.  |m 17213384X:Henn, Fritz A.  |d 60000  |e 60000PH17213384X  |k 0/60000/  |p 4 
998 |g 130680516  |a Vengeliene, Valentina  |m 130680516:Vengeliene, Valentina  |d 60000  |e 60000PV130680516  |k 0/60000/  |p 3 
998 |g 1045120928  |a Schmitt, Andrea  |m 1045120928:Schmitt, Andrea  |d 60000  |e 60000PS1045120928  |k 0/60000/  |p 2 
998 |g 121715221  |a Zink, Mathias  |m 121715221:Zink, Mathias  |d 60000  |e 60000PZ121715221  |k 0/60000/  |p 1  |x j 
999 |a KXP-PPN1738417360  |e 3798668442 
BIB |a Y 
SER |a journal 
JSO |a {"recId":"1738417360","note":["Gesehen am 11.11.2020"],"name":{"displayForm":["Mathias Zink, Andrea Schmitt, Valentina Vengeliene, Fritz A. Henn, Rainer Spanagel"]},"language":["eng"],"id":{"doi":["10.1097/01.ALC.0000145810.12545.B3"],"eki":["1738417360"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"origin":[{"dateIssuedDisp":"2004","dateIssuedKey":"2004"}],"relHost":[{"disp":"Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice culturesAlcoholism","recId":"329270001","pubHistory":["Vol. 1, no. 1 (January 1977)-vol 46, no 12 (December 2022)"],"note":["Gesehen am 28.09.2023","Fortsetzung der Druck-Ausgabe"],"language":["eng"],"id":{"eki":["329270001"],"doi":["10.1111/(ISSN)1530-0277"],"zdb":["2046886-6"],"issn":["1530-0277"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"publisherPlace":"Oxford [u.a.] ; Baltimore, Md. [u.a.] ; Oxford [u.a.]","dateIssuedDisp":"[1977-2022]","publisher":"Wiley-Blackwell ; Lippincott, Williams & Wilkins ; Blackwell"}],"part":{"pages":"1752-1757","text":"28(2004), 11, Seite 1752-1757","volume":"28","issue":"11","year":"2004","extent":"6"},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title_sort":"Alcoholism","subtitle":"clinical and experimental research ; the official journal of the Research Society on Alcoholism and the International Society for Biomedical Research on Alcoholism","title":"Alcoholism"}]}],"title":[{"title_sort":"Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures","title":"Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures"}],"person":[{"given":"Mathias","display":"Zink, Mathias","family":"Zink","role":"aut"},{"role":"aut","given":"Andrea","family":"Schmitt","display":"Schmitt, Andrea"},{"role":"aut","given":"Valentina","display":"Vengeliene, Valentina","family":"Vengeliene"},{"role":"aut","given":"Fritz A.","display":"Henn, Fritz A.","family":"Henn"},{"family":"Spanagel","display":"Spanagel, Rainer","given":"Rainer","role":"aut"}],"physDesc":[{"extent":"6 S."}]} 
SRT |a ZINKMATHIAETHANOLIND2004 

Similar Items