Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment
In previous studies CD8+ T cells specific for melanocyte antigens have been frequently found in melanoma patients responding to interleukin-2 (IL-2)-based therapies. In our study we analyzed the suitability of using circulating T cells from melanoma patients with clinical response after IL-2-based t...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
14 January 2002
|
| In: |
International journal of cancer
Year: 2002, Jahrgang: 98, Heft: 3, Pages: 409-414 |
| ISSN: | 1097-0215 |
| DOI: | https://doi.org/10.1002/ijc.10205 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/ijc.10205 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.10205 
|
| Verfasserangaben: | Carmen Scheibenbogen, Yuansheng Sun, Ulrich Keilholz, Mingxia Song, Stefan Stevanovic, Anne Marie Asemissen, Dirk Nagorsen, Eckhard Thiel, Hans-Georg Rammensee and Dirk Schadendorf |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1738426327 | ||
| 003 | DE-627 | ||
| 005 | 20230428161355.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201111s2002 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/ijc.10205 |2 doi | |
| 035 | |a (DE-627)1738426327 | ||
| 035 | |a (DE-599)KXP1738426327 | ||
| 035 | |a (OCoLC)1341376175 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Scheibenbogen, Carmen |d 1962- |e VerfasserIn |0 (DE-588)1108128629 |0 (DE-627)863747442 |0 (DE-576)176760598 |4 aut | |
| 245 | 1 | 0 | |a Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment |c Carmen Scheibenbogen, Yuansheng Sun, Ulrich Keilholz, Mingxia Song, Stefan Stevanovic, Anne Marie Asemissen, Dirk Nagorsen, Eckhard Thiel, Hans-Georg Rammensee and Dirk Schadendorf |
| 246 | 3 | 0 | |a two |
| 264 | 1 | |c 14 January 2002 | |
| 300 | |a 6 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 11.11.2020 | ||
| 520 | |a In previous studies CD8+ T cells specific for melanocyte antigens have been frequently found in melanoma patients responding to interleukin-2 (IL-2)-based therapies. In our study we analyzed the suitability of using circulating T cells from melanoma patients with clinical response after IL-2-based therapy to identify novel T-cell epitopes from defined tumor antigens. Using unstimulated peripheral blood mononuclear cells and the interferon-γ (IFN-γ) ELISPOT assay, we studied CD8+ T-cell responses against 5 peptides from the tumor antigen tyrosinase (Tyr) selected by epitope prediction using an HLA-A1-binding computer algorithm. T cells specifically secreting IFN-γ in response to 3 of these 5 peptides, namely, Tyr (454-463), Tyr (146-156) and Tyr (243-251), could be detected in 4 of 4 HLA-A1-positive patients with clinical response. In contrast, no T-cell responses against these peptides were seen in 6 HLA-A1-positive melanoma patients with progressive disease and in 8 healthy subjects. We could generate specific cytotoxic T lymphocytes (CTL) against Tyr (454-463) using peptide-pulsed autologous dendritic cells as antigen-presenting cells. The induced CTLs efficiently killed melanoma cells that express HLA-A1 and tyrosinase. The peptides Tyr (146-156) and Tyr (243-251) had recently been identified as CTL epitopes by other groups. Further ex vivo characterization of the T cells reactive against the novel epitope Tyr (454-463) in 1 patient by multicolor flow cytometry showed specific CD3+/CD8+/IFN-γ+ T cells with frequencies of up to 0.41% of the CD3+/CD8+ T-cell population. Most of this T-cell population also expressed granzyme B. Our data confirm that in patients with tumor regressions induced by immunotherapy or chemoimmunotherapy circulating T cells reactive with tyrosinase epitopes can frequently be detected. Peripheral blood T cells from such patients are a valuable source for screening peptides selected by epitope prediction This strategy facilitates the rapid identification of immunogenic T-cell epitopes that are probable targets of immune-mediated tumor rejection. © 2002 Wiley-Liss, Inc. | ||
| 650 | 4 | |a epitope prediction | |
| 650 | 4 | |a melanoma | |
| 650 | 4 | |a peptides | |
| 650 | 4 | |a tyrosinase | |
| 700 | 1 | |a Song, Mingxia |d 1965- |e VerfasserIn |0 (DE-588)131798189 |0 (DE-627)514263016 |0 (DE-576)255081332 |4 aut | |
| 700 | 1 | |a Schadendorf, Dirk |d 1960- |e VerfasserIn |0 (DE-588)11142576X |0 (DE-627)499566076 |0 (DE-576)289702275 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International journal of cancer |d Bognor Regis : Wiley-Liss, 1966 |g 98(2002), 3, Seite 409-414 |h Online-Ressource |w (DE-627)269532781 |w (DE-600)1474822-8 |w (DE-576)079876129 |x 1097-0215 |7 nnas |a Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment |
| 773 | 1 | 8 | |g volume:98 |g year:2002 |g number:3 |g pages:409-414 |g extent:6 |a Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment |
| 856 | 4 | 0 | |u https://doi.org/https://doi.org/10.1002/ijc.10205 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.10205 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201111 | ||
| 993 | |a Article | ||
| 994 | |a 2002 | ||
| 998 | |g 11142576X |a Schadendorf, Dirk |m 11142576X:Schadendorf, Dirk |d 50000 |e 50000PS11142576X |k 0/50000/ |p 5 | ||
| 998 | |g 131798189 |a Song, Mingxia |m 131798189:Song, Mingxia |d 50000 |e 50000PS131798189 |k 0/50000/ |p 4 | ||
| 999 | |a KXP-PPN1738426327 |e 3798692157 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2002","dateIssuedDisp":"14 January 2002"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"relHost":[{"titleTranslated":[{"translated":"Journal international du cancer"}],"recId":"269532781","titleAlt":[{"title":"Predictive oncology"}],"pubHistory":["1.1966 -"],"corporate":[{"role":"isb","display":"International Union against Cancer","roleDisplay":"Herausgebendes Organ"}],"language":["eng"],"type":{"bibl":"periodical","media":"Online-Ressource"},"origin":[{"publisher":"Wiley-Liss","publisherPlace":"Bognor Regis","dateIssuedKey":"1966","dateIssuedDisp":"1966-"}],"part":{"text":"98(2002), 3, Seite 409-414","volume":"98","issue":"3","year":"2002","extent":"6","pages":"409-414"},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"subtitle":"publication of the International Union against Cancer (UICC) = Journal international du cancer","title_sort":"International journal of cancer","title":"International journal of cancer"}],"id":{"doi":["10.1002/(ISSN)1097-0215"],"eki":["269532781"],"zdb":["1474822-8"],"issn":["1097-0215"]},"note":["Gesehen am 28.02.08","Darin: Predictive oncology"],"disp":"Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatmentInternational journal of cancer"}],"person":[{"roleDisplay":"VerfasserIn","family":"Scheibenbogen","given":"Carmen","display":"Scheibenbogen, Carmen","role":"aut"},{"given":"Mingxia","display":"Song, Mingxia","role":"aut","roleDisplay":"VerfasserIn","family":"Song"},{"display":"Schadendorf, Dirk","role":"aut","given":"Dirk","family":"Schadendorf","roleDisplay":"VerfasserIn"}],"name":{"displayForm":["Carmen Scheibenbogen, Yuansheng Sun, Ulrich Keilholz, Mingxia Song, Stefan Stevanovic, Anne Marie Asemissen, Dirk Nagorsen, Eckhard Thiel, Hans-Georg Rammensee and Dirk Schadendorf"]},"physDesc":[{"extent":"6 S."}],"note":["Gesehen am 11.11.2020"],"recId":"1738426327","id":{"doi":["10.1002/ijc.10205"],"eki":["1738426327"]},"title":[{"title":"Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment","title_sort":"Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment"}]} | ||
| SRT | |a SCHEIBENBOIDENTIFICA1420 | ||