Whole-genome sequencing disproves two suspected transmission events of blaNDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients
New Delhi metallo-β-lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of blaNDM between Enterobacterales and Pseudomonas aeruginosa, initially indicate...
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| Autori principali: | , , , , |
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| Natura: | Article (Journal) |
| Lingua: | inglese |
| Pubblicazione: |
9 July 2020
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| In: |
The journal of hospital infection
Year: 2020, Volume: 106, Fascicolo: 2, Pages: 372-375 |
| ISSN: | 1532-2939 |
| DOI: | 10.1016/j.jhin.2020.07.006 |
| Accesso online: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.jhin.2020.07.006 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0195670120303364 
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| Note sull'autore: | K. Kocer, S. Boutin, K. Probst, K. Heeg, D. Nurjadi |
| Riassunto: | New Delhi metallo-β-lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of blaNDM between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different blaNDM variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks. |
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| Descrizione del documento: | Im Titel ist "NDM" tiefgestellt Gesehen am 12.11.2020 |
| Descrizione fisica: | Online Resource |
| ISSN: | 1532-2939 |
| DOI: | 10.1016/j.jhin.2020.07.006 |