Detecting endogenous SUMO targets in mammalian cells and tissues
SUMOylation is a dynamic protein post-translational modification that regulates many eukaryotic proteins. Now a methodology using commercially available monoclonal antibodies coupled to MS analysis leads to the enrichment and identification of endogenous targets for SUMO1 and for SUMO2/3 in HeLa cel...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
17 March 2013
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| In: |
Nature structural & molecular biology
Year: 2013, Jahrgang: 20, Heft: 4, Pages: 525-531 |
| ISSN: | 1545-9985 |
| DOI: | 10.1038/nsmb.2526 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nsmb.2526 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nsmb.2526 
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| Verfasserangaben: | Janina Becker, Sina V Barysch, Samir Karaca, Claudia Dittner, He-Hsuan Hsiao, Mauricio Berriel Diaz, Stephan Herzig, Henning Urlaub & Frauke Melchior |
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| 520 | |a SUMOylation is a dynamic protein post-translational modification that regulates many eukaryotic proteins. Now a methodology using commercially available monoclonal antibodies coupled to MS analysis leads to the enrichment and identification of endogenous targets for SUMO1 and for SUMO2/3 in HeLa cells and mouse liver. This protocol can be adapted for other tissues and organs. | ||
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