Computerized image analysis of iron-stained macrophages

Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficient...

Full description

Saved in:
Bibliographic Details
Main Authors: Gente, Karolina (Author) , Schäfer, Sebastian Markus (Author) , Mall, Marcus A. (Author) , Muckenthaler, Martina (Author)
Format: Article (Journal)
Language:English
Published: 17 April 2013
In: Annals of hematology
Year: 2013, Volume: 92, Issue: 9, Pages: 1195-1199
ISSN:1432-0584
DOI:10.1007/s00277-013-1740-2
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00277-013-1740-2
Get full text
Author Notes:Karolina Benesova, Sebastian M. Schaefer, Marcus A. Mall, Martina U. Muckenthaler

MARC

LEADER 00000caa a2200000 c 4500
001 1740378768
003 DE-627
005 20220819031814.0
007 cr uuu---uuuuu
008 201123s2013 xx |||||o 00| ||eng c
024 7 |a 10.1007/s00277-013-1740-2  |2 doi 
035 |a (DE-627)1740378768 
035 |a (DE-599)KXP1740378768 
035 |a (OCoLC)1341378006 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Gente, Karolina  |d 1986-  |e VerfasserIn  |0 (DE-588)1066617481  |0 (DE-627)817734120  |0 (DE-576)426070216  |4 aut 
245 1 0 |a Computerized image analysis of iron-stained macrophages  |c Karolina Benesova, Sebastian M. Schaefer, Marcus A. Mall, Martina U. Muckenthaler 
264 1 |c 17 April 2013 
300 |a 5 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 23.11.2020 
520 |a Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficiently detected small differences in macrophage steady-state iron levels in Hfe−/− mice as well as inflammation-induced iron sequestration upon lipopolysaccharide instillation. In conclusion, computerized image analysis of single cells is a robust and reproducible tool suitable for iron measurements in small sample sets with limited cell yield. 
700 1 |a Schäfer, Sebastian Markus  |d 1985-  |e VerfasserIn  |0 (DE-588)1037798791  |0 (DE-627)756265444  |0 (DE-576)391831593  |4 aut 
700 1 |a Mall, Marcus A.  |d 1975-  |e VerfasserIn  |0 (DE-588)115507795  |0 (DE-627)691370532  |0 (DE-576)28992152X  |4 aut 
700 1 |a Muckenthaler, Martina  |e VerfasserIn  |0 (DE-588)1046124595  |0 (DE-627)775766496  |0 (DE-576)173146813  |4 aut 
773 0 8 |i Enthalten in  |t Annals of hematology  |d Berlin : Springer, 1991  |g 92(2013), 9, Seite 1195-1199  |h Online-Ressource  |w (DE-627)253389852  |w (DE-600)1458429-3  |w (DE-576)072283157  |x 1432-0584  |7 nnas  |a Computerized image analysis of iron-stained macrophages 
773 1 8 |g volume:92  |g year:2013  |g number:9  |g pages:1195-1199  |g extent:5  |a Computerized image analysis of iron-stained macrophages 
856 4 0 |u https://doi.org/10.1007/s00277-013-1740-2  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20201123 
993 |a Article 
994 |a 2013 
998 |g 1046124595  |a Muckenthaler, Martina  |m 1046124595:Muckenthaler, Martina  |d 910000  |d 910500  |e 910000PM1046124595  |e 910500PM1046124595  |k 0/910000/  |k 1/910000/910500/  |p 4  |y j 
998 |g 115507795  |a Mall, Marcus A.  |m 115507795:Mall, Marcus A.  |d 910000  |d 910500  |e 910000PM115507795  |e 910500PM115507795  |k 0/910000/  |k 1/910000/910500/  |p 3 
998 |g 1037798791  |a Schäfer, Sebastian Markus  |m 1037798791:Schäfer, Sebastian Markus  |d 50000  |e 50000PS1037798791  |k 0/50000/  |p 2 
998 |g 1066617481  |a Gente, Karolina  |m 1066617481:Gente, Karolina  |d 910000  |d 910100  |e 910000PG1066617481  |e 910100PG1066617481  |k 0/910000/  |k 1/910000/910100/  |p 1  |x j 
999 |a KXP-PPN1740378768  |e 3810425273 
BIB |a Y 
SER |a journal 
JSO |a {"note":["Gesehen am 23.11.2020"],"relHost":[{"id":{"eki":["253389852"],"issn":["1432-0584"],"zdb":["1458429-3"]},"type":{"media":"Online-Ressource","bibl":"periodical"},"origin":[{"dateIssuedKey":"1991","publisher":"Springer","dateIssuedDisp":"1991-","publisherPlace":"Berlin ; Heidelberg ; New York"}],"physDesc":[{"extent":"Online-Ressource"}],"titleAlt":[{"title":"Hematology"}],"pubHistory":["62, 1 (Februrary 1991)-"],"recId":"253389852","note":["Gesehen am 24.04.2025","Fortsetzung der Druck-Ausgabe","Ungezählte Beil. ab 80.2001: Supplement"],"disp":"Computerized image analysis of iron-stained macrophagesAnnals of hematology","language":["eng"],"part":{"issue":"9","volume":"92","pages":"1195-1199","text":"92(2013), 9, Seite 1195-1199","extent":"5","year":"2013"},"title":[{"title_sort":"Annals of hematology","title":"Annals of hematology","subtitle":"organ of: Gesellschaft für Thrombose- und Hämostaseforschung, DGHO, Deutsche Gesellschaft für Hämatologie und Onkologie e.V., ÖGHO, Österreichische Gesellschaft für Hämatologie und Onkologie"}]}],"language":["eng"],"title":[{"title":"Computerized image analysis of iron-stained macrophages","title_sort":"Computerized image analysis of iron-stained macrophages"}],"id":{"eki":["1740378768"],"doi":["10.1007/s00277-013-1740-2"]},"person":[{"given":"Karolina","role":"aut","family":"Gente","display":"Gente, Karolina"},{"display":"Schäfer, Sebastian Markus","family":"Schäfer","given":"Sebastian Markus","role":"aut"},{"family":"Mall","display":"Mall, Marcus A.","role":"aut","given":"Marcus A."},{"family":"Muckenthaler","display":"Muckenthaler, Martina","role":"aut","given":"Martina"}],"name":{"displayForm":["Karolina Benesova, Sebastian M. Schaefer, Marcus A. Mall, Martina U. Muckenthaler"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"physDesc":[{"extent":"5 S."}],"origin":[{"dateIssuedDisp":"17 April 2013","dateIssuedKey":"2013"}],"recId":"1740378768"} 
SRT |a GENTEKAROLCOMPUTERIZ1720