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Computerized image analysis of iron-stained macrophages

Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficient...

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Bibliographische Detailangaben
Hauptverfasser: Gente, Karolina (VerfasserIn) , Schäfer, Sebastian Markus (VerfasserIn) , Mall, Marcus A. (VerfasserIn) , Muckenthaler, Martina (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 17 April 2013
In: Annals of hematology
Year: 2013, Jahrgang: 92, Heft: 9, Pages: 1195-1199
ISSN:1432-0584
DOI:10.1007/s00277-013-1740-2
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00277-013-1740-2
Volltext
Verfasserangaben:Karolina Benesova, Sebastian M. Schaefer, Marcus A. Mall, Martina U. Muckenthaler
Beschreibung
Zusammenfassung:Analysis of iron levels in single cells is critical to understand the consequences of impaired regulation of iron homeostasis. Here we establish a method to analyze intracellular iron deposits by computerized image analysis of Prussian blue-stained alveolar macrophages as a test system. We efficiently detected small differences in macrophage steady-state iron levels in Hfe−/− mice as well as inflammation-induced iron sequestration upon lipopolysaccharide instillation. In conclusion, computerized image analysis of single cells is a robust and reproducible tool suitable for iron measurements in small sample sets with limited cell yield.
Beschreibung:Gesehen am 23.11.2020
Beschreibung:Online Resource
ISSN:1432-0584
DOI:10.1007/s00277-013-1740-2

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