Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin
Resistance to various anti-neoplastic agents is a common observation in clinical management of melanoma. The biologic mechanisms conferring these different drug-resistant phenotypes, including resistance against the commonly used anti-cancer drug cisplatin, are unclear. In order to elucidate the rol...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
8 December 2015
|
| In: |
The journal of investigative dermatology
Year: 2003, Jahrgang: 121, Heft: 1, Pages: 172-176 |
| ISSN: | 1523-1747 |
| DOI: | 10.1046/j.1523-1747.2003.12313.x |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1046/j.1523-1747.2003.12313.x Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S0022202X15303080 
|
| Verfasserangaben: | Bernd Liedert, Verena Materna, Dirk Schadendorf, Jürgen Thomale, Hermann Lage |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1740400658 | ||
| 003 | DE-627 | ||
| 005 | 20230428084931.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201123r20152003xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1046/j.1523-1747.2003.12313.x |2 doi | |
| 035 | |a (DE-627)1740400658 | ||
| 035 | |a (DE-599)KXP1740400658 | ||
| 035 | |a (OCoLC)1341377893 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Liedert, Bernd |d 1961- |e VerfasserIn |0 (DE-588)123361451 |0 (DE-627)706270789 |0 (DE-576)293675449 |4 aut | |
| 245 | 1 | 0 | |a Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin |c Bernd Liedert, Verena Materna, Dirk Schadendorf, Jürgen Thomale, Hermann Lage |
| 246 | 3 | 3 | |a Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G 2-arrest in melanoma cells resistant to cisplatin |
| 264 | 1 | |c 8 December 2015 | |
| 300 | |a 5 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Elektronische Reproduktion der Druckausgabe | ||
| 500 | |a Im Titel ist die Zahl 2 im Ausdruck "G2-arrest" tiefgestellt | ||
| 500 | |a Gesehen am 23.11.2020 | ||
| 520 | |a Resistance to various anti-neoplastic agents is a common observation in clinical management of melanoma. The biologic mechanisms conferring these different drug-resistant phenotypes, including resistance against the commonly used anti-cancer drug cisplatin, are unclear. In order to elucidate the role of the membrane adenosine triphosphate binding cassette-transporter cMOAT (canalicular multispecific anion transporter) (MRP2/ABCC2) in cisplatin resistance of melanoma, the expression of this protein was analyzed in the platinum drug-resistant cell line MeWo CIS 1. Cisplatinresistant melanoma cells showed a distinct overexpression of cMOAT on mRNA and protein level. This observation was accompanied by a reduced formation of platinum-induced intrastrand cross-links in the nuclear DNA measured by an immunocytologic assay. This decrease in DNA platination was accompanied by an accelerated re-entry into the cell cycle after the typical cisplatin-induced G2 arrest, and a resistance to undergo apoptosis. Kinetics of formation and elimination of platinum-DNA adducts suggest that the DNA repair capacity for Pt-d(GpG) adducts was not elevated in platinum drug-resistant melanoma cells. The decrease in platinum-DNA adduct formation in cisplatin-resistant melanoma cells was rather a reflection of the protecting activity of the transporter cMOAT. In conclusion, the functional inhibition of cMOAT might be a promising strategy in the reversal of resistance to platinum-based anti-cancer drugs in human melanoma. | ||
| 534 | |c 2003 | ||
| 650 | 4 | |a ABCC2 | |
| 650 | 4 | |a cisplatin | |
| 650 | 4 | |a cMOAT | |
| 650 | 4 | |a drug resistance | |
| 650 | 4 | |a melanoma | |
| 650 | 4 | |a MeWo | |
| 650 | 4 | |a MRP2 | |
| 700 | 1 | |a Schadendorf, Dirk |d 1960- |e VerfasserIn |0 (DE-588)11142576X |0 (DE-627)499566076 |0 (DE-576)289702275 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of investigative dermatology |d Amsterdam : Elsevier, 1938 |g 121(2003), 1, Seite 172-176 |h Online-Ressource |w (DE-627)320457990 |w (DE-600)2006902-9 |w (DE-576)09114289X |x 1523-1747 |7 nnas |a Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin |
| 773 | 1 | 8 | |g volume:121 |g year:2003 |g number:1 |g pages:172-176 |g extent:5 |a Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin |
| 856 | 4 | 0 | |u https://doi.org/10.1046/j.1523-1747.2003.12313.x |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S0022202X15303080 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201123 | ||
| 993 | |a Article | ||
| 994 | |a 2003 | ||
| 998 | |g 11142576X |a Schadendorf, Dirk |m 11142576X:Schadendorf, Dirk |d 50000 |e 50000PS11142576X |k 0/50000/ |p 3 | ||
| 999 | |a KXP-PPN1740400658 |e 3810496138 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"recId":"1740400658","titleAlt":[{"title":"Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G 2-arrest in melanoma cells resistant to cisplatin"}],"person":[{"given":"Bernd","display":"Liedert, Bernd","role":"aut","family":"Liedert","roleDisplay":"VerfasserIn"},{"given":"Dirk","role":"aut","display":"Schadendorf, Dirk","roleDisplay":"VerfasserIn","family":"Schadendorf"}],"relHost":[{"disp":"Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatinThe journal of investigative dermatology","note":["Gesehen am 16.02.16"],"title":[{"title_sort":"journal of investigative dermatology","title":"The journal of investigative dermatology"}],"id":{"eki":["320457990"],"zdb":["2006902-9"],"issn":["1523-1747"]},"part":{"pages":"172-176","year":"2003","extent":"5","issue":"1","volume":"121","text":"121(2003), 1, Seite 172-176"},"physDesc":[{"extent":"Online-Ressource"}],"name":{"displayForm":["Society for Investigative Dermatology ; European Society for Dermatological Research"]},"language":["eng"],"origin":[{"publisherPlace":"Amsterdam ; Oxford [u.a.] ; [Erscheinungsort nicht ermittelbar] ; Basingstoke [u.a.]","dateIssuedKey":"1938","dateIssuedDisp":"1938-","publisher":"Elsevier ; Blackwell ; Wiley-Blackwell ; Nature Publishing Group"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"corporate":[{"role":"isb","display":"Society for Investigative Dermatology","roleDisplay":"Herausgebendes Organ"}],"titleAlt":[{"title":"an international journal for research in cutaneous biology"}],"pubHistory":["1.1938 -"],"recId":"320457990"}],"title":[{"title":"Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin","title_sort":"Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin"}],"id":{"doi":["10.1046/j.1523-1747.2003.12313.x"],"eki":["1740400658"]},"note":["Elektronische Reproduktion der Druckausgabe","Im Titel ist die Zahl 2 im Ausdruck \"G2-arrest\" tiefgestellt","Gesehen am 23.11.2020"],"language":["eng"],"origin":[{"dateIssuedDisp":"8 December 2015","dateIssuedKey":"2015"}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Bernd Liedert, Verena Materna, Dirk Schadendorf, Jürgen Thomale, Hermann Lage"]},"physDesc":[{"extent":"5 S."}]} | ||
| SRT | |a LIEDERTBEROVEREXPRES8201 | ||