Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma
Background: Head and neck squamous cell carcinomas (HNSCC) are phenotypically and molecularly heterogeneous and frequently develop therapy resistance. Reliable patient-derived 3D tumor models are urgently needed to further study the complex pathogenesis of these tumors and to overcome treatment fail...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
18 August 2020
|
| In: |
Cancers
Year: 2020, Jahrgang: 12, Heft: 8 |
| ISSN: | 2072-6694 |
| DOI: | 10.3390/cancers12082330 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/cancers12082330 Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/2072-6694/12/8/2330 
|
| Verfasserangaben: | Luca Engelmann, Julia Thierauf, Natalia Koerich Laureano, Hans-Juergen Stark, Elena-Sophie Prigge, Dominik Horn, Kolja Freier, Niels Grabe, Chao Rong, Philippe Federspil, Karim Zaoui, Peter K. Plinkert, Nicole Rotter, Magnus von Knebel Doeberitz, Jochen Hess and Annette Affolter |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1742725082 | ||
| 003 | DE-627 | ||
| 005 | 20220819052819.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 201214s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/cancers12082330 |2 doi | |
| 035 | |a (DE-627)1742725082 | ||
| 035 | |a (DE-599)KXP1742725082 | ||
| 035 | |a (OCoLC)1341383761 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Engelmann, Luca |d 1995- |e VerfasserIn |0 (DE-588)1223386503 |0 (DE-627)1742726224 |4 aut | |
| 245 | 1 | 0 | |a Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma |c Luca Engelmann, Julia Thierauf, Natalia Koerich Laureano, Hans-Juergen Stark, Elena-Sophie Prigge, Dominik Horn, Kolja Freier, Niels Grabe, Chao Rong, Philippe Federspil, Karim Zaoui, Peter K. Plinkert, Nicole Rotter, Magnus von Knebel Doeberitz, Jochen Hess and Annette Affolter |
| 264 | 1 | |c 18 August 2020 | |
| 300 | |a 20 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 14.12.2020 | ||
| 520 | |a Background: Head and neck squamous cell carcinomas (HNSCC) are phenotypically and molecularly heterogeneous and frequently develop therapy resistance. Reliable patient-derived 3D tumor models are urgently needed to further study the complex pathogenesis of these tumors and to overcome treatment failure. Methods: We developed a three-dimensional organotypic co-culture (3D-OTC) model for HNSCC that maintains the architecture and cell composition of the individual tumor. A dermal equivalent (DE), composed of healthy human-derived fibroblasts and viscose fibers, served as a scaffold for the patient sample. DEs were co-cultivated with 13 vital HNSCC explants (non-human papillomavirus (HPV) driven, n = 7; HPV-driven, n = 6). Fractionated irradiation was applied to 5 samples (non-HPV-driven, n = 2; HPV-driven n = 3). To evaluate expression of ki-67, cleaved caspase-3, pan-cytokeratin, p16INK4a, CD45, ∝smooth muscle actin and vimentin over time, immunohistochemistry and immunofluorescence staining were performed Patient checkup data were collected for up to 32 months after first diagnosis. Results: All non-HPV-driven 3D-OTCs encompassed proliferative cancer cells during cultivation for up to 21 days. Proliferation indices of primaries and 3D-OTCs were comparable and consistent over time. Overall, tumor explants displayed heterogeneous growth patterns (i.e., invasive, expansive, silent). Cancer-associated fibroblasts and leukocytes could be detected for up to 21 days. HPV DNA was detectable in both primary and 3D-OTCs (day 14) of HPV-driven tumors. However, p16INK4a expression levels were varying. Morphological alterations and radioresistant tumor cells were detected in 3D-OTC after fractionated irradiation in HPV-driven and non-driven samples. Conclusions: Our 3D-OTC model for HNSCC supports cancer cell survival and proliferation in their original microenvironment. The model enables investigation of invasive cancer growth and might, in the future, serve as a platform to perform sensitivity testing upon treatment to predict therapy response. | ||
| 650 | 4 | |a 3D organotypic co-culture model | |
| 650 | 4 | |a HNSCC | |
| 650 | 4 | |a HPV | |
| 650 | 4 | |a invasion | |
| 700 | 1 | |a Thierauf, Julia |e VerfasserIn |0 (DE-588)1068770597 |0 (DE-627)820629472 |0 (DE-576)426753771 |4 aut | |
| 700 | 1 | |a Laureano, Natalia K. |e VerfasserIn |0 (DE-588)1192313615 |0 (DE-627)1670624536 |4 aut | |
| 700 | 1 | |a Stark, Hans-Jürgen |e VerfasserIn |0 (DE-588)1217623221 |0 (DE-627)1731806809 |4 aut | |
| 700 | 1 | |a Prigge, Elena-Sophie |e VerfasserIn |0 (DE-588)1070611352 |0 (DE-627)824110757 |0 (DE-576)431628653 |4 aut | |
| 700 | 1 | |a Horn, Dominik |d 1984- |e VerfasserIn |0 (DE-588)1036568628 |0 (DE-627)750560045 |0 (DE-576)384807445 |4 aut | |
| 700 | 1 | |a Freier, Kolja |d 1973- |e VerfasserIn |0 (DE-588)128789972 |0 (DE-627)380633396 |0 (DE-576)18748144X |4 aut | |
| 700 | 1 | |a Grabe, Niels |e VerfasserIn |0 (DE-588)123210615 |0 (DE-627)716920697 |0 (DE-576)364937602 |4 aut | |
| 700 | 1 | |a Rong, Chao |d 1986- |e VerfasserIn |0 (DE-588)1127247433 |0 (DE-627)881669199 |0 (DE-576)485140799 |4 aut | |
| 700 | 1 | |a Federspil, Philippe A. |d 1967- |e VerfasserIn |0 (DE-588)1032667982 |0 (DE-627)73862070X |0 (DE-576)176922237 |4 aut | |
| 700 | 1 | |a Plath, Karim |d 1977- |e VerfasserIn |0 (DE-588)140238719 |0 (DE-627)703610813 |0 (DE-576)314596607 |4 aut | |
| 700 | 1 | |a Plinkert, Peter K. |e VerfasserIn |0 (DE-588)1032658509 |0 (DE-627)739933043 |0 (DE-576)169008908 |4 aut | |
| 700 | 1 | |a Rotter, Nicole |d 1970- |e VerfasserIn |0 (DE-588)118085638 |0 (DE-627)079235514 |0 (DE-576)178653888 |4 aut | |
| 700 | 1 | |a Knebel Doeberitz, Magnus von |e VerfasserIn |0 (DE-588)1022165291 |0 (DE-627)71693289X |0 (DE-576)364934239 |4 aut | |
| 700 | 1 | |a Heß, Jochen |d 1971- |e VerfasserIn |0 (DE-588)121902390 |0 (DE-627)705696057 |0 (DE-576)292983417 |4 aut | |
| 700 | 1 | |a Affolter, Annette |d 1977- |e VerfasserIn |0 (DE-588)124918417 |0 (DE-627)368073726 |0 (DE-576)294568190 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cancers |d Basel : MDPI, 2009 |g 12(2020,8) Artikel-Nummer 2330, 20 Seiten |h Online-Ressource |w (DE-627)614095670 |w (DE-600)2527080-1 |w (DE-576)313958548 |x 2072-6694 |7 nnas |a Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma |
| 773 | 1 | 8 | |g volume:12 |g year:2020 |g number:8 |g extent:20 |a Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma |
| 856 | 4 | 0 | |u https://doi.org/10.3390/cancers12082330 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.mdpi.com/2072-6694/12/8/2330 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20201214 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 124918417 |a Affolter, Annette |m 124918417:Affolter, Annette |d 60000 |d 62100 |e 60000PA124918417 |e 62100PA124918417 |k 0/60000/ |k 1/60000/62100/ |p 16 |y j | ||
| 998 | |g 121902390 |a Heß, Jochen |m 121902390:Heß, Jochen |d 910000 |d 911000 |d 50000 |e 910000PH121902390 |e 911000PH121902390 |e 50000PH121902390 |k 0/910000/ |k 1/910000/911000/ |k 0/50000/ |p 15 | ||
| 998 | |g 1022165291 |a Knebel Doeberitz, Magnus von |m 1022165291:Knebel Doeberitz, Magnus von |d 910000 |d 912000 |e 910000PK1022165291 |e 912000PK1022165291 |k 0/910000/ |k 1/910000/912000/ |p 14 | ||
| 998 | |g 118085638 |a Rotter, Nicole |m 118085638:Rotter, Nicole |d 60000 |d 62100 |e 60000PR118085638 |e 62100PR118085638 |k 0/60000/ |k 1/60000/62100/ |p 13 | ||
| 998 | |g 1032658509 |a Plinkert, Peter K. |m 1032658509:Plinkert, Peter K. |d 910000 |d 911000 |e 910000PP1032658509 |e 911000PP1032658509 |k 0/910000/ |k 1/910000/911000/ |p 12 | ||
| 998 | |g 140238719 |a Plath, Karim |m 140238719:Plath, Karim |d 910000 |d 911000 |d 50000 |e 910000PP140238719 |e 911000PP140238719 |e 50000PP140238719 |k 0/910000/ |k 1/910000/911000/ |k 0/50000/ |p 11 | ||
| 998 | |g 1032667982 |a Federspil, Philippe A. |m 1032667982:Federspil, Philippe A. |d 910000 |d 911000 |d 50000 |e 910000PF1032667982 |e 911000PF1032667982 |e 50000PF1032667982 |k 0/910000/ |k 1/910000/911000/ |k 0/50000/ |p 10 | ||
| 998 | |g 123210615 |a Grabe, Niels |m 123210615:Grabe, Niels |d 910000 |d 910100 |d 50000 |e 910000PG123210615 |e 910100PG123210615 |e 50000PG123210615 |k 0/910000/ |k 1/910000/910100/ |k 0/50000/ |p 8 | ||
| 998 | |g 128789972 |a Freier, Kolja |m 128789972:Freier, Kolja |d 50000 |e 50000PF128789972 |k 0/50000/ |p 7 | ||
| 998 | |g 1036568628 |a Horn, Dominik |m 1036568628:Horn, Dominik |d 50000 |e 50000PH1036568628 |k 0/50000/ |p 6 | ||
| 998 | |g 1070611352 |a Prigge, Elena-Sophie |m 1070611352:Prigge, Elena-Sophie |d 910000 |d 912000 |e 910000PP1070611352 |e 912000PP1070611352 |k 0/910000/ |k 1/910000/912000/ |p 5 | ||
| 998 | |g 1217623221 |a Stark, Hans-Jürgen |m 1217623221:Stark, Hans-Jürgen |d 50000 |e 50000PS1217623221 |k 0/50000/ |p 4 | ||
| 998 | |g 1068770597 |a Thierauf, Julia |m 1068770597:Thierauf, Julia |d 910000 |d 911000 |e 910000PT1068770597 |e 911000PT1068770597 |k 0/910000/ |k 1/910000/911000/ |p 2 | ||
| 998 | |g 1223386503 |a Engelmann, Luca |m 1223386503:Engelmann, Luca |d 50000 |e 50000PE1223386503 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1742725082 |e 382536559X | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"display":"Engelmann, Luca","family":"Engelmann","role":"aut","given":"Luca"},{"family":"Thierauf","role":"aut","display":"Thierauf, Julia","given":"Julia"},{"given":"Natalia K.","family":"Laureano","role":"aut","display":"Laureano, Natalia K."},{"family":"Stark","role":"aut","display":"Stark, Hans-Jürgen","given":"Hans-Jürgen"},{"given":"Elena-Sophie","role":"aut","family":"Prigge","display":"Prigge, Elena-Sophie"},{"given":"Dominik","family":"Horn","role":"aut","display":"Horn, Dominik"},{"display":"Freier, Kolja","family":"Freier","role":"aut","given":"Kolja"},{"given":"Niels","display":"Grabe, Niels","family":"Grabe","role":"aut"},{"family":"Rong","role":"aut","display":"Rong, Chao","given":"Chao"},{"display":"Federspil, Philippe A.","family":"Federspil","role":"aut","given":"Philippe A."},{"family":"Plath","role":"aut","display":"Plath, Karim","given":"Karim"},{"given":"Peter K.","family":"Plinkert","role":"aut","display":"Plinkert, Peter K."},{"given":"Nicole","family":"Rotter","role":"aut","display":"Rotter, Nicole"},{"family":"Knebel Doeberitz","role":"aut","display":"Knebel Doeberitz, Magnus von","given":"Magnus von"},{"given":"Jochen","display":"Heß, Jochen","family":"Heß","role":"aut"},{"given":"Annette","display":"Affolter, Annette","role":"aut","family":"Affolter"}],"note":["Gesehen am 14.12.2020"],"language":["eng"],"name":{"displayForm":["Luca Engelmann, Julia Thierauf, Natalia Koerich Laureano, Hans-Juergen Stark, Elena-Sophie Prigge, Dominik Horn, Kolja Freier, Niels Grabe, Chao Rong, Philippe Federspil, Karim Zaoui, Peter K. Plinkert, Nicole Rotter, Magnus von Knebel Doeberitz, Jochen Hess and Annette Affolter"]},"origin":[{"dateIssuedKey":"2020","dateIssuedDisp":"18 August 2020"}],"id":{"eki":["1742725082"],"doi":["10.3390/cancers12082330"]},"recId":"1742725082","type":{"bibl":"article-journal","media":"Online-Ressource"},"physDesc":[{"extent":"20 S."}],"title":[{"title_sort":"Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma","title":"Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinoma"}],"relHost":[{"origin":[{"dateIssuedDisp":"2009-","publisherPlace":"Basel","dateIssuedKey":"2009","publisher":"MDPI"}],"name":{"displayForm":["Molecular Diversity Preservation International (MDPI)"]},"disp":"Organotypic co-cultures as a novel 3D model for head and neck squamous cell carcinomaCancers","type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"614095670","id":{"eki":["614095670"],"zdb":["2527080-1"],"issn":["2072-6694"]},"language":["eng"],"note":["Gesehen am 27.05.2020"],"pubHistory":["1.2009 -"],"part":{"volume":"12","issue":"8","year":"2020","text":"12(2020,8) Artikel-Nummer 2330, 20 Seiten","extent":"20"},"title":[{"title":"Cancers","title_sort":"Cancers"}],"physDesc":[{"extent":"Online-Ressource"}]}]} | ||
| SRT | |a ENGELMANNLORGANOTYPI1820 | ||