Identification of contaminating fungal DNA sequences in zymolyase
When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
March 1, 1999
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| In: |
Journal of clinical microbiology
Year: 1999, Volume: 37, Issue: 3, Pages: 830-831 |
| ISSN: | 1098-660X |
| DOI: | 10.1128/JCM.37.3.830-831.1999 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/JCM.37.3.830-831.1999 Verlag, lizenzpflichtig, Volltext: https://jcm.asm.org/content/37/3/830 
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| Author Notes: | Dagmar Rimek, Amar P. Garg, Walter H. Haas, Reinhard Kappe |
| Summary: | When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex andKluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA. |
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| Item Description: | Gesehen am 16.12.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1098-660X |
| DOI: | 10.1128/JCM.37.3.830-831.1999 |