Cover Image

The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophila

Transfer RNA (tRNA) fragmentation in response to stress conditions has been described in many organisms. tRNA fragments have been found in association with small interfering RNA (siRNA) components, but the biological role of these interactions remains unclear. We report here that the tRNA methyltran...

Full description

Saved in:
Bibliographic Details
Main Authors: Durdevic, Zeljko (Author) , Mobin, Mehrpouya Balaghy (Author) , Hanna, Katharina (Author) , Lyko, Frank (Author) , Schaefer, Matthias R. (Author)
Format: Article (Journal)
Language:English
Published: September 5, 2013
In: Cell reports
Year: 2013, Volume: 4, Issue: 5, Pages: 931-937
ISSN:2211-1247
DOI:10.1016/j.celrep.2013.07.046
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.celrep.2013.07.046
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124713004087
Get full text
Author Notes:Zeljko Durdevic, Mehrpouya Balaghy Mobin, Katharina Hanna, Frank Lyko, Matthias Schaefer

MARC

LEADER 00000caa a2200000 c 4500
001 1743958048
003 DE-627
005 20250618121730.0
007 cr uuu---uuuuu
008 210105s2013 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.celrep.2013.07.046  |2 doi 
035 |a (DE-627)1743958048 
035 |a (DE-599)KXP1743958048 
035 |a (OCoLC)1341384345 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 32  |2 sdnb 
100 1 |a Durdevic, Zeljko  |e VerfasserIn  |0 (DE-588)1037319583  |0 (DE-627)755444728  |0 (DE-576)391317873  |4 aut 
245 1 4 |a The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophila  |c Zeljko Durdevic, Mehrpouya Balaghy Mobin, Katharina Hanna, Frank Lyko, Matthias Schaefer 
264 1 |c September 5, 2013 
300 |a 7 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 05.01.2021 
520 |a Transfer RNA (tRNA) fragmentation in response to stress conditions has been described in many organisms. tRNA fragments have been found in association with small interfering RNA (siRNA) components, but the biological role of these interactions remains unclear. We report here that the tRNA methyltransferase Dnmt2 is essential for efficient Dicer-2 (Dcr-2) function in Drosophila. Using small RNA (sRNA) sequencing, we confirmed that Dnmt2 limits the extent of tRNA fragmentation during the heat-shock response. tRNAs as well as tRNA fragments serve as Dcr-2 substrates, and Dcr-2 degrades tRNA-derived sequences, especially under heat-shock conditions. tRNA-derived RNAs are able to inhibit Dcr-2 activity on long double-stranded RNAs (dsRNAs). Consequently, heat-shocked Dnmt2 mutant animals accumulate dsRNAs, produce fewer siRNAs, and show misregulation of siRNA pathway-dependent genes. These results reveal the impact of tRNA fragmentation on siRNA pathways and implicate tRNA modifications in the regulation of sRNA homeostasis during the heat-shock response. 
700 1 |a Mobin, Mehrpouya Balaghy  |d 1986-  |e VerfasserIn  |0 (DE-588)1104272105  |0 (DE-627)861748859  |0 (DE-576)470907428  |4 aut 
700 1 |a Hanna, Katharina  |e VerfasserIn  |4 aut 
700 1 |a Lyko, Frank  |e VerfasserIn  |0 (DE-588)1106120361  |0 (DE-627)862900018  |0 (DE-576)473860597  |4 aut 
700 1 |a Schaefer, Matthias R.  |d 1968-  |e VerfasserIn  |0 (DE-588)1263055907  |0 (DE-627)1811034276  |4 aut 
773 0 8 |i Enthalten in  |t Cell reports  |d Maryland Heights, MO : Cell Press, 2012  |g 4(2013), 5, Seite 931-937  |h Online-Ressource  |w (DE-627)684964562  |w (DE-600)2649101-1  |w (DE-576)358411572  |x 2211-1247  |7 nnas  |a The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophila 
773 1 8 |g volume:4  |g year:2013  |g number:5  |g pages:931-937  |g extent:7  |a The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophila 
856 4 0 |u https://doi.org/10.1016/j.celrep.2013.07.046  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u http://www.sciencedirect.com/science/article/pii/S2211124713004087  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210105 
993 |a Article 
994 |a 2013 
998 |g 1263055907  |a Schaefer, Matthias R.  |m 1263055907:Schaefer, Matthias R.  |d 50000  |e 50000PS1263055907  |k 0/50000/  |p 7 
998 |g 1106120361  |a Lyko, Frank  |m 1106120361:Lyko, Frank  |d 140000  |e 140000PL1106120361  |k 0/140000/  |p 6 
999 |a KXP-PPN1743958048  |e 3830516401 
BIB |a Y 
SER |a journal 
JSO |a {"title":[{"title_sort":"RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophila","title":"The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophila"}],"relHost":[{"language":["eng"],"id":{"eki":["684964562"],"issn":["2211-1247"],"zdb":["2649101-1"]},"type":{"bibl":"periodical","media":"Online-Ressource"},"physDesc":[{"extent":"Online-Ressource"}],"part":{"year":"2013","extent":"7","volume":"4","text":"4(2013), 5, Seite 931-937","pages":"931-937","issue":"5"},"pubHistory":["1.2012 -"],"title":[{"title":"Cell reports","title_sort":"Cell reports"}],"recId":"684964562","disp":"The RNA methyltransferase Dnmt2 is required for efficient Dicer-2-dependent siRNA pathway activity in drosophilaCell reports","origin":[{"dateIssuedKey":"2012","publisherPlace":"Maryland Heights, MO ; [New York, NY]","publisher":"Cell Press ; Elsevier","dateIssuedDisp":"2012-"}]}],"physDesc":[{"extent":"7 S."}],"id":{"eki":["1743958048"],"doi":["10.1016/j.celrep.2013.07.046"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"person":[{"given":"Zeljko","family":"Durdevic","role":"aut","display":"Durdevic, Zeljko"},{"role":"aut","display":"Mobin, Mehrpouya Balaghy","given":"Mehrpouya Balaghy","family":"Mobin"},{"family":"Hanna","given":"Katharina","display":"Hanna, Katharina","role":"aut"},{"family":"Lyko","given":"Frank","display":"Lyko, Frank","role":"aut"},{"family":"Schaefer","given":"Matthias R.","display":"Schaefer, Matthias R.","role":"aut"}],"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"September 5, 2013"}],"note":["Gesehen am 05.01.2021"],"name":{"displayForm":["Zeljko Durdevic, Mehrpouya Balaghy Mobin, Katharina Hanna, Frank Lyko, Matthias Schaefer"]},"recId":"1743958048"} 
SRT |a DURDEVICZERNAMETHYLT5201 

Similar Items