RNA expression profiling from FACS-isolated cells of the Drosophila intestine
This unit describes a protocol for the isolation of Drosophila intestinal cell populations for the purpose of cell type-specific transcriptome profiling. A method to select a cell type of interest labeled with green or yellow fluorescent protein (GFP, YFP) by making use of the GAL4-UAS bipartite sys...
Gespeichert in:
| Hauptverfasser: | , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
13 November 2013
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| In: |
Current protocols in stem cell biology
Year: 2013, Jahrgang: 27, Heft: 1, Pages: 2F.2.1-2F.2.12 |
| ISSN: | 1938-8969 |
| DOI: | https://doi.org/10.1002/9780470151808.sc02f02s27 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/https://doi.org/10.1002/9780470151808.sc02f02s27 Verlag, lizenzpflichtig, Volltext: https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/9780470151808.sc02f02s27 
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| Verfasserangaben: | Devanjali Dutta, Jinyi Xiang, Bruce A. Edgar |
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| 520 | |a This unit describes a protocol for the isolation of Drosophila intestinal cell populations for the purpose of cell type-specific transcriptome profiling. A method to select a cell type of interest labeled with green or yellow fluorescent protein (GFP, YFP) by making use of the GAL4-UAS bipartite system and fluorescent-activated cell sorting (FACS) is presented. Total RNA is isolated from the sorted cells and linear RNA amplification is used to obtain sufficient amounts of high-quality RNA for analysis by microarray, RT-PCR, or RNA sequencing. This method will be useful for quantitative transcriptome comparison across intestinal cell types under normal and various experimental conditions. Curr. Protoc. Stem Cell Biol. 27:2F.2.1-2F.2.12. © 2013 by John Wiley & Sons, Inc. | ||
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| 650 | 4 | |a Drosophila intestinal stem cells | |
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| 650 | 4 | |a fluorescent-activated cell sorting (FACS) | |
| 650 | 4 | |a transcriptome profiling/RNASeq | |
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