Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies
Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation-dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2-infected human...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
November 17, 2020
|
| In: |
Cell host and microbe
Year: 2020, Jahrgang: 28, Heft: 6, Pages: 853-866.e5 |
| ISSN: | 1934-6069 |
| DOI: | 10.1016/j.chom.2020.11.003 |
| Online-Zugang: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.chom.2020.11.003 Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S193131282030620X 
|
| Verfasserangaben: | Mirko Cortese, Ji-Young Lee, Berati Cerikan, Christopher J. Neufeldt, Viola M. J. Oorschot, Sebastian Köhrer, Julian Hennies, Nicole L. Schieber, Paolo Ronchi, Giulia Mizzon, Inés Romero-Brey, Rachel Santarella-Mellwig, Martin Schorb, Mandy Boermel, Karel Mocaer, Marianne S. Beckwith, Rachel M. Templin, Viktoriia Gross, Constantin Pape, Christian Tischer, Jamie Frankish, Natalie K. Horvat, Vibor Laketa, Megan Stanifer, Steeve Boulant, Alessia Ruggieri, Laurent Chatel-Chaix, Yannick Schwab, and Ralf Bartenschlager |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1744400113 | ||
| 003 | DE-627 | ||
| 005 | 20251222142912.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210112s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.chom.2020.11.003 |2 doi | |
| 035 | |a (DE-627)1744400113 | ||
| 035 | |a (DE-599)KXP1744400113 | ||
| 035 | |a (OCoLC)1341385111 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Cortese, Mirko |e VerfasserIn |0 (DE-588)1073720225 |0 (DE-627)829364609 |0 (DE-576)435287699 |4 aut | |
| 245 | 1 | 0 | |a Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies |c Mirko Cortese, Ji-Young Lee, Berati Cerikan, Christopher J. Neufeldt, Viola M. J. Oorschot, Sebastian Köhrer, Julian Hennies, Nicole L. Schieber, Paolo Ronchi, Giulia Mizzon, Inés Romero-Brey, Rachel Santarella-Mellwig, Martin Schorb, Mandy Boermel, Karel Mocaer, Marianne S. Beckwith, Rachel M. Templin, Viktoriia Gross, Constantin Pape, Christian Tischer, Jamie Frankish, Natalie K. Horvat, Vibor Laketa, Megan Stanifer, Steeve Boulant, Alessia Ruggieri, Laurent Chatel-Chaix, Yannick Schwab, and Ralf Bartenschlager |
| 264 | 1 | |c November 17, 2020 | |
| 300 | |a 20 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.01.2021 | ||
| 520 | |a Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation-dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2-infected human lung epithelial cells. We report 3D electron microscopy reconstructions of whole cells and subcellular compartments, revealing extensive fragmentation of the Golgi apparatus, alteration of the mitochondrial network and recruitment of peroxisomes to viral replication organelles formed by clusters of double-membrane vesicles (DMVs). These are tethered to the endoplasmic reticulum, providing insights into DMV biogenesis and spatial coordination of SARS-CoV-2 replication. Live cell imaging combined with an infection sensor reveals profound remodeling of cytoskeleton elements. Pharmacological inhibition of their dynamics suppresses SARS-CoV-2 replication. We thus report insights into virus-induced cytopathic effects and provide alongside a comprehensive publicly available repository of 3D datasets of SARS-CoV-2-infected cells for download and smooth online visualization. | ||
| 650 | 4 | |a coronavirus | |
| 650 | 4 | |a cytoskeleton | |
| 650 | 4 | |a electron tomography | |
| 650 | 4 | |a FIB-SEM | |
| 650 | 4 | |a Golgi | |
| 650 | 4 | |a intermediate filaments | |
| 650 | 4 | |a live cell imaging | |
| 650 | 4 | |a membrane remodeling | |
| 650 | 4 | |a peroxisomes | |
| 650 | 4 | |a viral replication organelles | |
| 700 | 1 | |a Lee, Ji Young |e VerfasserIn |0 (DE-588)1058274570 |0 (DE-627)79668037X |0 (DE-576)414323610 |4 aut | |
| 700 | 1 | |a Cerikan, Berati |e VerfasserIn |0 (DE-588)1126652776 |0 (DE-627)881234761 |0 (DE-576)484627627 |4 aut | |
| 700 | 1 | |a Neufeldt, Christopher |e VerfasserIn |0 (DE-588)113060795X |0 (DE-627)88481954X |0 (DE-576)486862925 |4 aut | |
| 700 | 1 | |a Oorschot, Viola M. J. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Köhrer, Sebastian |e VerfasserIn |0 (DE-588)1311448853 |0 (DE-627)1871457076 |4 aut | |
| 700 | 1 | |a Hennies, Julian |d 1986- |e VerfasserIn |0 (DE-588)1297746155 |0 (DE-627)1853915807 |4 aut | |
| 700 | 1 | |a Schieber, Nicole L. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Ronchi, Paolo |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mizzon, Giulia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Romero-Brey, Inés |e VerfasserIn |4 aut | |
| 700 | 1 | |a Santarella-Mellwig, Rachel |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schorb, Martin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Boermel, Mandy |e VerfasserIn |4 aut | |
| 700 | 1 | |a Mocaer, Karel |d 1995- |e VerfasserIn |0 (DE-588)132449316X |0 (DE-627)1884374948 |4 aut | |
| 700 | 1 | |a Beckwith, Marianne S. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Templin, Rachel M. |e VerfasserIn |4 aut | |
| 700 | 1 | |a Gross, Viktoriia |e VerfasserIn |4 aut | |
| 700 | 1 | |a Pape, Constantin |d 1990- |e VerfasserIn |0 (DE-588)1130605213 |0 (DE-627)884813975 |0 (DE-576)486857379 |4 aut | |
| 700 | 1 | |a Tischer, Christian |e VerfasserIn |4 aut | |
| 700 | 1 | |a Frankish, Jamie |e VerfasserIn |4 aut | |
| 700 | 1 | |a Horvat, Natalie |d 1988- |e VerfasserIn |0 (DE-588)1219684287 |0 (DE-627)1735662356 |4 aut | |
| 700 | 1 | |a Laketa, Vibor |e VerfasserIn |0 (DE-588)132778068 |0 (DE-627)526931523 |0 (DE-576)299406830 |4 aut | |
| 700 | 1 | |a Stanifer, Megan |e VerfasserIn |0 (DE-588)1130687643 |0 (DE-627)884888835 |0 (DE-576)486922367 |4 aut | |
| 700 | 1 | |a Boulant, Steeve |e VerfasserIn |0 (DE-588)1130687430 |0 (DE-627)884888509 |0 (DE-576)48692243X |4 aut | |
| 700 | 1 | |a Ruggieri, Alessia |e VerfasserIn |0 (DE-588)1068888504 |0 (DE-627)82097952X |0 (DE-576)428250114 |4 aut | |
| 700 | 1 | |a Chatel-Chaix, Laurent |e VerfasserIn |4 aut | |
| 700 | 1 | |a Schwab, Yannick |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bartenschlager, Ralf |d 1958- |e VerfasserIn |0 (DE-588)1058097989 |0 (DE-627)796390509 |0 (DE-576)168706067 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell host and microbe |d Amsterdam [u.a.] : Elsevier, 2007 |g 28(2020), 6, Seite 853-866.e5 |h Online-Ressource |w (DE-627)527572993 |w (DE-600)2276339-9 |w (DE-576)264425944 |x 1934-6069 |7 nnas |a Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies |
| 773 | 1 | 8 | |g volume:28 |g year:2020 |g number:6 |g pages:853-866.e5 |g extent:20 |a Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.chom.2020.11.003 |x Resolving-System |x Verlag |z lizenzpflichtig |3 Volltext |7 1 |
| 856 | 4 | 0 | |u http://www.sciencedirect.com/science/article/pii/S193131282030620X |x Verlag |z lizenzpflichtig |3 Volltext |7 1 |
| 951 | |a AR | ||
| 992 | |a 20210112 | ||
| 993 | |a Article | ||
| 994 | |a 2020 | ||
| 998 | |g 1058097989 |a Bartenschlager, Ralf |m 1058097989:Bartenschlager, Ralf |d 910000 |d 911700 |e 910000PB1058097989 |e 911700PB1058097989 |k 0/910000/ |k 1/910000/911700/ |p 29 |y j | ||
| 998 | |g 1068888504 |a Ruggieri, Alessia |m 1068888504:Ruggieri, Alessia |d 910000 |d 911700 |e 910000PR1068888504 |e 911700PR1068888504 |k 0/910000/ |k 1/910000/911700/ |p 26 | ||
| 998 | |g 1130687430 |a Boulant, Steeve |m 1130687430:Boulant, Steeve |d 910000 |d 911700 |e 910000PB1130687430 |e 911700PB1130687430 |k 0/910000/ |k 1/910000/911700/ |p 25 | ||
| 998 | |g 1130687643 |a Stanifer, Megan |m 1130687643:Stanifer, Megan |d 910000 |d 911700 |e 910000PS1130687643 |e 911700PS1130687643 |k 0/910000/ |k 1/910000/911700/ |p 24 | ||
| 998 | |g 132778068 |a Laketa, Vibor |m 132778068:Laketa, Vibor |d 910000 |d 911700 |e 910000PL132778068 |e 911700PL132778068 |k 0/910000/ |k 1/910000/911700/ |p 23 | ||
| 998 | |g 1219684287 |a Horvat, Natalie |m 1219684287:Horvat, Natalie |d 140000 |e 140000PH1219684287 |k 0/140000/ |p 22 | ||
| 998 | |g 1130605213 |a Pape, Constantin |m 1130605213:Pape, Constantin |d 130000 |e 130000PP1130605213 |k 0/130000/ |p 19 | ||
| 998 | |g 132449316X |a Mocaer, Karel |m 132449316X:Mocaer, Karel |d 140000 |e 140000PM132449316X |k 0/140000/ |p 15 | ||
| 998 | |g 1297746155 |a Hennies, Julian |m 1297746155:Hennies, Julian |d 140000 |d 140001 |e 140000PH1297746155 |e 140001PH1297746155 |k 0/140000/ |k 1/140000/140001/ |p 7 | ||
| 998 | |g 1311448853 |a Köhrer, Sebastian |m 1311448853:Köhrer, Sebastian |d 50000 |e 50000PK1311448853 |k 0/50000/ |p 6 | ||
| 998 | |g 113060795X |a Neufeldt, Christopher |m 113060795X:Neufeldt, Christopher |d 910000 |d 911700 |e 910000PN113060795X |e 911700PN113060795X |k 0/910000/ |k 1/910000/911700/ |p 4 | ||
| 998 | |g 1126652776 |a Cerikan, Berati |m 1126652776:Cerikan, Berati |d 910000 |d 911700 |e 910000PC1126652776 |e 911700PC1126652776 |k 0/910000/ |k 1/910000/911700/ |p 3 | ||
| 998 | |g 1058274570 |a Lee, Ji Young |m 1058274570:Lee, Ji Young |d 910000 |d 911700 |e 910000PL1058274570 |e 911700PL1058274570 |k 0/910000/ |k 1/910000/911700/ |p 2 | ||
| 998 | |g 1073720225 |a Cortese, Mirko |m 1073720225:Cortese, Mirko |d 910000 |d 911700 |e 910000PC1073720225 |e 911700PC1073720225 |k 0/910000/ |k 1/910000/911700/ |p 1 |x j | ||
| 999 | |a KXP-PPN1744400113 |e 3834488917 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"title":[{"title":"Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies","title_sort":"Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies"}],"person":[{"role":"aut","display":"Cortese, Mirko","given":"Mirko","family":"Cortese"},{"given":"Ji Young","family":"Lee","display":"Lee, Ji Young","role":"aut"},{"family":"Cerikan","given":"Berati","display":"Cerikan, Berati","role":"aut"},{"display":"Neufeldt, Christopher","given":"Christopher","family":"Neufeldt","role":"aut"},{"role":"aut","given":"Viola M. J.","family":"Oorschot","display":"Oorschot, Viola M. J."},{"given":"Sebastian","family":"Köhrer","display":"Köhrer, Sebastian","role":"aut"},{"role":"aut","given":"Julian","family":"Hennies","display":"Hennies, Julian"},{"role":"aut","display":"Schieber, Nicole L.","family":"Schieber","given":"Nicole L."},{"display":"Ronchi, Paolo","family":"Ronchi","given":"Paolo","role":"aut"},{"display":"Mizzon, Giulia","given":"Giulia","family":"Mizzon","role":"aut"},{"display":"Romero-Brey, Inés","family":"Romero-Brey","given":"Inés","role":"aut"},{"given":"Rachel","family":"Santarella-Mellwig","display":"Santarella-Mellwig, Rachel","role":"aut"},{"role":"aut","display":"Schorb, Martin","given":"Martin","family":"Schorb"},{"role":"aut","display":"Boermel, Mandy","family":"Boermel","given":"Mandy"},{"role":"aut","display":"Mocaer, Karel","given":"Karel","family":"Mocaer"},{"display":"Beckwith, Marianne S.","given":"Marianne S.","family":"Beckwith","role":"aut"},{"given":"Rachel M.","family":"Templin","display":"Templin, Rachel M.","role":"aut"},{"role":"aut","family":"Gross","given":"Viktoriia","display":"Gross, Viktoriia"},{"role":"aut","family":"Pape","given":"Constantin","display":"Pape, Constantin"},{"role":"aut","family":"Tischer","given":"Christian","display":"Tischer, Christian"},{"display":"Frankish, Jamie","family":"Frankish","given":"Jamie","role":"aut"},{"display":"Horvat, Natalie","given":"Natalie","family":"Horvat","role":"aut"},{"role":"aut","display":"Laketa, Vibor","family":"Laketa","given":"Vibor"},{"display":"Stanifer, Megan","family":"Stanifer","given":"Megan","role":"aut"},{"role":"aut","family":"Boulant","given":"Steeve","display":"Boulant, Steeve"},{"role":"aut","family":"Ruggieri","given":"Alessia","display":"Ruggieri, Alessia"},{"display":"Chatel-Chaix, Laurent","given":"Laurent","family":"Chatel-Chaix","role":"aut"},{"given":"Yannick","family":"Schwab","display":"Schwab, Yannick","role":"aut"},{"given":"Ralf","family":"Bartenschlager","display":"Bartenschlager, Ralf","role":"aut"}],"origin":[{"dateIssuedDisp":"November 17, 2020","dateIssuedKey":"2020"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"note":["Gesehen am 12.01.2021"],"id":{"doi":["10.1016/j.chom.2020.11.003"],"eki":["1744400113"]},"recId":"1744400113","name":{"displayForm":["Mirko Cortese, Ji-Young Lee, Berati Cerikan, Christopher J. Neufeldt, Viola M. J. Oorschot, Sebastian Köhrer, Julian Hennies, Nicole L. Schieber, Paolo Ronchi, Giulia Mizzon, Inés Romero-Brey, Rachel Santarella-Mellwig, Martin Schorb, Mandy Boermel, Karel Mocaer, Marianne S. Beckwith, Rachel M. Templin, Viktoriia Gross, Constantin Pape, Christian Tischer, Jamie Frankish, Natalie K. Horvat, Vibor Laketa, Megan Stanifer, Steeve Boulant, Alessia Ruggieri, Laurent Chatel-Chaix, Yannick Schwab, and Ralf Bartenschlager"]},"relHost":[{"type":{"bibl":"periodical","media":"Online-Ressource"},"origin":[{"dateIssuedKey":"2007","dateIssuedDisp":"2007-","publisher":"Elsevier","publisherPlace":"Amsterdam [u.a.]"}],"pubHistory":["1.2007 -"],"title":[{"title":"Cell host and microbe","title_sort":"Cell host and microbe"}],"note":["Gesehen am 20.11.2020"],"recId":"527572993","id":{"eki":["527572993"],"zdb":["2276339-9"],"issn":["1934-6069"]},"language":["eng"],"part":{"text":"28(2020), 6, Seite 853-866.e5","year":"2020","issue":"6","extent":"20","volume":"28","pages":"853-866.e5"},"physDesc":[{"extent":"Online-Ressource"}],"disp":"Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologiesCell host and microbe"}],"physDesc":[{"extent":"20 S."}],"language":["eng"]} | ||
| SRT | |a CORTESEMIRINTEGRATIV1720 | ||