A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation
Mammalian nonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance mechanism that degrades mRNAs containing premature translation termination codons. Phosphorylation of the essential NMD effector UPF1 by the phosphoinositide-3-kinase-like kinase (PIKK) SMG-1 is a key step in NMD and occurs wh...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
30 June 2015
|
| In: |
Nucleic acids research
Year: 2015, Jahrgang: 43, Heft: 15, Pages: 7600-7611 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkv668 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gkv668
|
| Verfasserangaben: | Aurélien Deniaud, Manikandan Karuppasamy, Thomas Bock, Simonas Masiulis, Karine Huard, Frédéric Garzoni, Kathrin Kerschgens, Matthias W. Hentze, Andreas E. Kulozik, Martin Beck, Gabriele Neu-Yilik and Christiane Schaffitzel |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1744429022 | ||
| 003 | DE-627 | ||
| 005 | 20220819072439.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210112s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1093/nar/gkv668 |2 doi | |
| 035 | |a (DE-627)1744429022 | ||
| 035 | |a (DE-599)KXP1744429022 | ||
| 035 | |a (OCoLC)1341384991 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Deniaud, Aurélien |e VerfasserIn |0 (DE-588)1224917421 |0 (DE-627)1744441413 |4 aut | |
| 245 | 1 | 2 | |a A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation |c Aurélien Deniaud, Manikandan Karuppasamy, Thomas Bock, Simonas Masiulis, Karine Huard, Frédéric Garzoni, Kathrin Kerschgens, Matthias W. Hentze, Andreas E. Kulozik, Martin Beck, Gabriele Neu-Yilik and Christiane Schaffitzel |
| 264 | 1 | |c 30 June 2015 | |
| 300 | |a 12 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 12.01.2021 | ||
| 520 | |a Mammalian nonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance mechanism that degrades mRNAs containing premature translation termination codons. Phosphorylation of the essential NMD effector UPF1 by the phosphoinositide-3-kinase-like kinase (PIKK) SMG-1 is a key step in NMD and occurs when SMG-1, its two regulatory factors SMG-8 and SMG-9, and UPF1 form a complex at a terminating ribosome. Electron cryo-microscopy of the SMG-1-8-9-UPF1 complex shows the head and arm architecture characteristic of PIKKs and reveals different states of UPF1 docking. UPF1 is recruited to the SMG-1 kinase domain and C-terminal insertion domain, inducing an opening of the head domain that provides access to the active site. SMG-8 and SMG-9 interact with the SMG-1 C-insertion and promote high-affinity UPF1 binding to SMG-1-8-9, as well as decelerated SMG-1 kinase activity and enhanced stringency of phosphorylation site selection. The presence of UPF2 destabilizes the SMG-1-8-9-UPF1 complex leading to substrate release. Our results suggest an intricate molecular network of SMG-8, SMG-9 and the SMG-1 C-insertion domain that governs UPF1 substrate recruitment and phosphorylation by SMG-1 kinase, an event that is central to trigger mRNA decay. | ||
| 700 | 1 | |a Karuppasamy, Manikandan |e VerfasserIn |4 aut | |
| 700 | 1 | |a Bock, Thomas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Masiulis, Simonas |e VerfasserIn |4 aut | |
| 700 | 1 | |a Huard, Karine |e VerfasserIn |4 aut | |
| 700 | 1 | |a Garzoni, Frédéric |e VerfasserIn |4 aut | |
| 700 | 1 | |a Kerschgens, Kathrin |e VerfasserIn |0 (DE-588)1013393287 |0 (DE-627)664166431 |0 (DE-576)347032494 |4 aut | |
| 700 | 1 | |a Hentze, Matthias W. |d 1960- |e VerfasserIn |0 (DE-588)143450395 |0 (DE-627)646106775 |0 (DE-576)337135754 |4 aut | |
| 700 | 1 | |a Kulozik, Andreas |d 1959- |e VerfasserIn |0 (DE-588)1025736567 |0 (DE-627)723892032 |0 (DE-576)167091638 |4 aut | |
| 700 | 1 | |a Beck, Martin |e VerfasserIn |4 aut | |
| 700 | 1 | |a Neu-Yilik, Gabriele |d 1958- |e VerfasserIn |0 (DE-588)1076384889 |0 (DE-627)834823233 |0 (DE-576)165318333 |4 aut | |
| 700 | 1 | |a Schaffitzel, Christiane |e VerfasserIn |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nucleic acids research |d Oxford : Oxford Univ. Press, 1974 |g 43(2015), 15, Seite 7600-7611 |h Online-Ressource |w (DE-627)26813250X |w (DE-600)1472175-2 |w (DE-576)07760878X |x 1362-4962 |7 nnas |a A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation |
| 773 | 1 | 8 | |g volume:43 |g year:2015 |g number:15 |g pages:7600-7611 |g extent:12 |a A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation |
| 856 | 4 | 0 | |u https://doi.org/10.1093/nar/gkv668 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210112 | ||
| 993 | |a Article | ||
| 994 | |a 2015 | ||
| 998 | |g 1076384889 |a Neu-Yilik, Gabriele |m 1076384889:Neu-Yilik, Gabriele |d 910000 |d 910500 |e 910000PN1076384889 |e 910500PN1076384889 |k 0/910000/ |k 1/910000/910500/ |p 10 | ||
| 998 | |g 1025736567 |a Kulozik, Andreas |m 1025736567:Kulozik, Andreas |d 910000 |d 910500 |e 910000PK1025736567 |e 910500PK1025736567 |k 0/910000/ |k 1/910000/910500/ |p 8 | ||
| 998 | |g 143450395 |a Hentze, Matthias W. |m 143450395:Hentze, Matthias W. |d 50000 |e 50000PH143450395 |k 0/50000/ |p 7 | ||
| 998 | |g 1013393287 |a Kerschgens, Kathrin |m 1013393287:Kerschgens, Kathrin |d 50000 |e 50000PK1013393287 |k 0/50000/ |p 6 | ||
| 999 | |a KXP-PPN1744429022 |e 3834558443 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 12.01.2021"],"relHost":[{"disp":"A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylationNucleic acids research","recId":"26813250X","part":{"extent":"12","text":"43(2015), 15, Seite 7600-7611","volume":"43","pages":"7600-7611","year":"2015","issue":"15"},"origin":[{"publisherPlace":"Oxford","dateIssuedDisp":"1974-","dateIssuedKey":"1974","publisher":"Oxford Univ. Press"}],"id":{"eki":["26813250X"],"zdb":["1472175-2"],"issn":["1362-4962"]},"language":["eng"],"pubHistory":["1.1974 -"],"physDesc":[{"extent":"Online-Ressource"}],"note":["Gesehen am 15.09.2025","Ungezählte Beil.: Database issue; Web server issue"],"titleAlt":[{"title":"NAR online"},{"title":"NAR"},{"title":"Database issue"},{"title":"Web server issue"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title":"Nucleic acids research","title_sort":"Nucleic acids research"}]}],"title":[{"title_sort":"network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation","title":"A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation"}],"person":[{"given":"Aurélien","role":"aut","family":"Deniaud","display":"Deniaud, Aurélien"},{"display":"Karuppasamy, Manikandan","role":"aut","given":"Manikandan","family":"Karuppasamy"},{"display":"Bock, Thomas","given":"Thomas","role":"aut","family":"Bock"},{"role":"aut","given":"Simonas","family":"Masiulis","display":"Masiulis, Simonas"},{"display":"Huard, Karine","family":"Huard","role":"aut","given":"Karine"},{"display":"Garzoni, Frédéric","family":"Garzoni","role":"aut","given":"Frédéric"},{"display":"Kerschgens, Kathrin","family":"Kerschgens","given":"Kathrin","role":"aut"},{"display":"Hentze, Matthias W.","given":"Matthias W.","role":"aut","family":"Hentze"},{"display":"Kulozik, Andreas","family":"Kulozik","given":"Andreas","role":"aut"},{"display":"Beck, Martin","family":"Beck","given":"Martin","role":"aut"},{"display":"Neu-Yilik, Gabriele","family":"Neu-Yilik","given":"Gabriele","role":"aut"},{"display":"Schaffitzel, Christiane","role":"aut","given":"Christiane","family":"Schaffitzel"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"physDesc":[{"extent":"12 S."}],"origin":[{"dateIssuedKey":"2015","dateIssuedDisp":"30 June 2015"}],"id":{"doi":["10.1093/nar/gkv668"],"eki":["1744429022"]},"recId":"1744429022","name":{"displayForm":["Aurélien Deniaud, Manikandan Karuppasamy, Thomas Bock, Simonas Masiulis, Karine Huard, Frédéric Garzoni, Kathrin Kerschgens, Matthias W. Hentze, Andreas E. Kulozik, Martin Beck, Gabriele Neu-Yilik and Christiane Schaffitzel"]}} | ||
| SRT | |a DENIAUDAURNETWORKOFS3020 | ||