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Chemogenetic control of nanobodies

We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approa...

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Hauptverfasser: Farrants, Helen (VerfasserIn) , Tarnawski, Miroslaw (VerfasserIn) , Müller, Thorsten G. (VerfasserIn) , Otsuka, Shotaro (VerfasserIn) , Hiblot, Julien (VerfasserIn) , Koch, Birgit (VerfasserIn) , Kueblbeck, Moritz (VerfasserIn) , Kräusslich, Hans-Georg (VerfasserIn) , Ellenberg, Jan (VerfasserIn) , Johnsson, Kai (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 17 February 2020
In: Nature methods
Year: 2020, Jahrgang: 17, Heft: 3, Pages: 279-282
ISSN:1548-7105
DOI:10.1038/s41592-020-0746-7
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41592-020-0746-7
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41592-020-0746-7
Volltext
Verfasserangaben:Helen Farrants, Miroslaw Tarnawski, Thorsten G. Müller, Shotaro Otsuka, Julien Hiblot, Birgit Koch, Moritz Kueblbeck, Hans-Georg Kräusslich, Jan Ellenberg and Kai Johnsson
Beschreibung
Zusammenfassung:We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
Beschreibung:Gesehen am 13.01.2021
Beschreibung:Online Resource
ISSN:1548-7105
DOI:10.1038/s41592-020-0746-7

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