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Mutational Analysis of the Nsa2 N-Terminus Reveals Its Essential Role in Ribosomal 60S Subunit Assembly

The ribosome assembly factor Nsa2 is part of the Rea1-Rsa4-Nsa2 interconnected relay on nuclear pre-60S particles that is essential for 60S ribosome biogenesis. Cryo-EM structures depict Nsa2 docked via its C-terminal β-barrel domain to nuclear pre-60S particles, whereas the extended N-term...

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Hauptverfasser: Paternoga, Helge (VerfasserIn) , Früh, Alexander (VerfasserIn) , Kunze, Ruth (VerfasserIn) , Bradatsch, Bettina (VerfasserIn) , Baßler, Jochen (VerfasserIn) , Hurt, Ed (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 30 November 2020
In: International journal of molecular sciences
Year: 2020, Jahrgang: 21, Heft: 23, Pages: 1-15
ISSN:1422-0067
DOI:10.3390/ijms21239108
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3390/ijms21239108
Verlag, lizenzpflichtig, Volltext: https://www.mdpi.com/1422-0067/21/23/9108
Volltext
Verfasserangaben:Helge Paternoga, Alexander Früh, Ruth Kunze, Bettina Bradatsch, Jochen Baßler and Ed Hurt
Beschreibung
Zusammenfassung:The ribosome assembly factor Nsa2 is part of the Rea1-Rsa4-Nsa2 interconnected relay on nuclear pre-60S particles that is essential for 60S ribosome biogenesis. Cryo-EM structures depict Nsa2 docked via its C-terminal β-barrel domain to nuclear pre-60S particles, whereas the extended N-terminus, consisting of three α-helical segments, meanders between various 25S rRNA helices with the extreme N-terminus in close vicinity to the Nog1 GTPase center. Here, we tested whether this unappreciated proximity between Nsa2 and Nog1 is of functional importance. Our findings demonstrate that a conservative mutation, Nsa2 Q3N, abolished cell growth and impaired 60S biogenesis. Subsequent genetic and biochemical analyses verified that the Nsa2 N-terminus is required to target Nsa2 to early pre-60S particles. However, overexpression of the Nsa2 N-terminus abolished cytoplasmic recycling of the Nog1 GTPase, and both Nog1 and the Nsa2-N (1-58) construct, but not the respective Nsa2-N (1-58) Q3N mutant, were found arrested on late cytoplasmic pre-60S particles. These findings point to specific roles of the different Nsa2 domains for 60S ribosome biogenesis.
Beschreibung:Gesehen am 18.01.2021
Beschreibung:Online Resource
ISSN:1422-0067
DOI:10.3390/ijms21239108

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