Buchumschlag

Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis

Methods: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects. Protein and mRNA (in PBMC) levels for IL-27 and IL-35 (IL27, EBI3, IL12A subunits) as well as IL-27 receptor (IL6ST and IL27RA subunits) were assesse...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
1. Verfasser: Ringkowski, Sabine (VerfasserIn)
Weitere Verfasser: Herth, Felix (BerichterstatterIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 1 July 2016
In: Respiratory medicine
Year: 2016, Jahrgang: 117, Pages: 243-253
ISSN:1532-3064
DOI:10.1016/j.rmed.2016.06.021
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.rmed.2016.06.021
Verlag, lizenzpflichtig, Volltext: https://linkinghub.elsevier.com/retrieve/pii/S0954611116301470
Volltext
Verfasserangaben:Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J.F. Herth, Paul S. Thomas, Cristan Herbert

MARC

LEADER 00000caa a2200000 c 4500
001 174514563X
003 DE-627
005 20230426074240.0
007 cr uuu---uuuuu
008 210121s2016 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.rmed.2016.06.021  |2 doi 
035 |a (DE-627)174514563X 
035 |a (DE-599)KXP174514563X 
035 |a (OCoLC)1341389040 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Ringkowski, Sabine  |d 1988-  |e VerfasserIn  |0 (DE-588)1219586854  |0 (DE-627)1735517933  |4 aut 
245 1 0 |a Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis  |c Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J.F. Herth, Paul S. Thomas, Cristan Herbert 
246 3 0 |a twenty-seven 
264 1 |c 1 July 2016 
300 |a 11 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 21.01.2021 
520 |a Methods: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects. Protein and mRNA (in PBMC) levels for IL-27 and IL-35 (IL27, EBI3, IL12A subunits) as well as IL-27 receptor (IL6ST and IL27RA subunits) were assessed spontaneously and following direct (LPS) and indirect (anti-CD3/28 activation beads) macrophage stimulation using RT- PCR, ELISA and flow cytometry. - Results: Following stimulation with LPS, PBMC of patients with sarcoidosis displayed significantly enhanced expression of IL27 and EBI3 mRNA (p ¼ 0.020 and p ¼ 0.037 respectively) compared to PBMCs from healthy controls. There was also significantly enhanced production of IL-27 by PBMC from patients with sarcoidosis compared to healthy controls in response to LPS stimulation (p ¼ 0.027). IL6ST mRNA and IL6ST protein were significantly lower in patients with sarcoidosis (mRNA p ¼ 0.0002; MFI p ¼ 0.0015) whilst IL27RA protein levels were significantly higher in patients with sarcoidosis compared to healthy controls (MFI p < 0.0001). Plasma IL-35 protein levels did not differ between control and sarcoidosis subjects (p ¼ 0.23). - Conclusion: These results suggest there may be exaggerated activation of IL-27 signalling in response to LPS in sarcoidosis. 
700 1 |a Herth, Felix  |0 (DE-588)1016095236  |0 (DE-627)705477568  |0 (DE-576)351509925  |4 oth 
773 0 8 |i Enthalten in  |t Respiratory medicine  |d Burlington, Mass. : Harcourt, 1999  |g 117(2016), Seite 243-253  |h Online-Ressource  |w (DE-627)320420973  |w (DE-600)2002557-9  |w (DE-576)103746870  |x 1532-3064  |7 nnas  |a Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis 
773 1 8 |g volume:117  |g year:2016  |g pages:243-253  |g extent:11  |a Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis 
856 4 0 |u https://doi.org/10.1016/j.rmed.2016.06.021  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u https://linkinghub.elsevier.com/retrieve/pii/S0954611116301470  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210121 
993 |a Article 
994 |a 2016 
998 |g 1016095236  |a Herth, Felix  |m 1016095236:Herth, Felix  |d 910000  |d 950000  |d 950900  |d 50000  |e 910000PH1016095236  |e 950000PH1016095236  |e 950900PH1016095236  |e 50000PH1016095236  |k 0/910000/  |k 1/910000/950000/  |k 2/910000/950000/950900/  |k 0/50000/  |p 5 
998 |g 1219586854  |a Ringkowski, Sabine  |m 1219586854:Ringkowski, Sabine  |d 50000  |e 50000PR1219586854  |k 0/50000/  |p 1  |x j 
999 |a KXP-PPN174514563X  |e 3840503329 
BIB |a Y 
SER |a journal 
JSO |a {"person":[{"display":"Ringkowski, Sabine","given":"Sabine","family":"Ringkowski","role":"aut"},{"role":"oth","family":"Herth","display":"Herth, Felix","given":"Felix"}],"recId":"174514563X","origin":[{"dateIssuedDisp":"1 July 2016","dateIssuedKey":"2016"}],"name":{"displayForm":["Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J.F. Herth, Paul S. Thomas, Cristan Herbert"]},"language":["eng"],"title":[{"title":"Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis","title_sort":"Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis"}],"physDesc":[{"extent":"11 S."}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"id":{"eki":["174514563X"],"doi":["10.1016/j.rmed.2016.06.021"]},"relHost":[{"pubHistory":["83.1989 -"],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"320420973","disp":"Enhanced LPS-induced activation of IL-27 signalling in sarcoidosisRespiratory medicine","language":["eng"],"physDesc":[{"extent":"Online-Ressource"}],"id":{"zdb":["2002557-9"],"issn":["1532-3064"],"eki":["320420973"]},"part":{"volume":"117","text":"117(2016), Seite 243-253","extent":"11","year":"2016","pages":"243-253"},"note":["Gesehen am 21.02.20"],"origin":[{"publisher":"Harcourt","dateIssuedDisp":"1999-","dateIssuedKey":"1999","publisherPlace":"Burlington, Mass."}],"title":[{"title":"Respiratory medicine","title_sort":"Respiratory medicine"}]}],"note":["Gesehen am 21.01.2021"]} 
SRT |a RINGKOWSKIENHANCEDLP1201 

Ähnliche Einträge