Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice
Purpose: The frequently expressed differentiation antigen tyrosinase-related protein-2 (TRP-2) has repeatedly been described as a target of spontaneous cytotoxic T-cell responses in melanoma patients, suggesting that it might be an ideal candidate antigen for T cell-based immunotherapy. As a prerequ...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
July 20, 2005
|
| In: |
Clinical cancer research
Year: 2005, Volume: 11, Issue: 14, Pages: 5241-5247 |
| ISSN: | 1557-3265 |
| DOI: | 10.1158/1078-0432.CCR-05-0170 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1158/1078-0432.CCR-05-0170 Verlag, lizenzpflichtig, Volltext: https://clincancerres.aacrjournals.org/content/11/14/5241 
|
| Author Notes: | Annette Paschen, Mingxia Song, Wolfram Osen, Xuan Duc Nguyen, Jan Mueller-Berghaus, Daniela Fink, Nadine Daniel, Mariel Donzeau, Wolfgang Nagel, Harald Kropshofer, and Dirk Schadendorf |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1745294872 | ||
| 003 | DE-627 | ||
| 005 | 20220819083344.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210122s2005 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1158/1078-0432.CCR-05-0170 |2 doi | |
| 035 | |a (DE-627)1745294872 | ||
| 035 | |a (DE-599)KXP1745294872 | ||
| 035 | |a (OCoLC)1341389205 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Paschen, Annette |e VerfasserIn |0 (DE-588)1076261353 |0 (DE-627)834584263 |0 (DE-576)445109475 |4 aut | |
| 245 | 1 | 0 | |a Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice |c Annette Paschen, Mingxia Song, Wolfram Osen, Xuan Duc Nguyen, Jan Mueller-Berghaus, Daniela Fink, Nadine Daniel, Mariel Donzeau, Wolfgang Nagel, Harald Kropshofer, and Dirk Schadendorf |
| 246 | 3 | 0 | |a four two one |
| 246 | 3 | 3 | |a Detection of spontaneous CD 4 + T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice |
| 264 | 1 | |c July 20, 2005 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Im Titel ist das Zeichen "+" hochgestellt | ||
| 500 | |a Gesehen am 22.01.2021 | ||
| 520 | |a Purpose: The frequently expressed differentiation antigen tyrosinase-related protein-2 (TRP-2) has repeatedly been described as a target of spontaneous cytotoxic T-cell responses in melanoma patients, suggesting that it might be an ideal candidate antigen for T cell-based immunotherapy. As a prerequisite for immunization, T-cell epitopes have to be identified. Whereas a number of HLA class I-presented TRP-2-derived epitopes are known, information about HLA class II-presented antigenic ligands recognized by CD4+ T helper (Th) cells is limited. - Experimental Design: The search for TRP-2-derived Th epitopes was carried out by competitive in vitro peptide binding studies with predicted HLA-DRB1*0301 ligands in combination with peptide and protein immunizations of HLA-DRB1*0301 transgenic mice. In vivo selected candidate epitopes were subsequently verified for their immunogenicity in human T-cell cultures. - Results: This strategy led to the characterization of TRP-260-74 as an HLA-DRB1*0301-restricted Th epitope. Importantly, TRP-260-74-reactive human CD4+ Th cell lines, specifically recognizing target cells loaded with recombinant TRP-2 protein, could be established by repeated peptide stimulation of peripheral blood lymphocytes from several HLA-DRB1*03+ melanoma patients. Even short-term peptide stimulation of patients' peripheral blood lymphocytes showed the presence of TRP-260-74-reactive T cells, suggesting that these T cells were already activated in vivo. - Conclusion: Peptide TRP-260-74 might be a useful tool for the improvement of immunotherapy and immune monitoring of melanoma patients. | ||
| 700 | 1 | |a Song, Mingxia |d 1965- |e VerfasserIn |0 (DE-588)131798189 |0 (DE-627)514263016 |0 (DE-576)255081332 |4 aut | |
| 700 | 1 | |a Osen, Wolfram |d 1963- |e VerfasserIn |0 (DE-588)114590789X |0 (DE-627)1007012056 |0 (DE-576)172955033 |4 aut | |
| 700 | 1 | |a Nguyen, Xuan-Duc |d 1966- |e VerfasserIn |0 (DE-588)115750924 |0 (DE-627)691563241 |0 (DE-576)177546042 |4 aut | |
| 700 | 1 | |a Schadendorf, Dirk |d 1960- |e VerfasserIn |0 (DE-588)11142576X |0 (DE-627)499566076 |0 (DE-576)289702275 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Clinical cancer research |d Philadelphia, Pa. [u.a.] : AACR, 1995 |g 11(2005), 14, Seite 5241-5247 |h Online-Ressource |w (DE-627)325489971 |w (DE-600)2036787-9 |w (DE-576)094502234 |x 1557-3265 |7 nnas |a Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice |
| 773 | 1 | 8 | |g volume:11 |g year:2005 |g number:14 |g pages:5241-5247 |g extent:7 |a Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice |
| 856 | 4 | 0 | |u https://doi.org/10.1158/1078-0432.CCR-05-0170 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://clincancerres.aacrjournals.org/content/11/14/5241 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210122 | ||
| 993 | |a Article | ||
| 994 | |a 2005 | ||
| 998 | |g 11142576X |a Schadendorf, Dirk |m 11142576X:Schadendorf, Dirk |d 50000 |e 50000PS11142576X |k 0/50000/ |p 11 |y j | ||
| 998 | |g 115750924 |a Nguyen, Xuan-Duc |m 115750924:Nguyen, Xuan-Duc |d 60000 |d 63500 |e 60000PN115750924 |e 63500PN115750924 |k 0/60000/ |k 1/60000/63500/ |p 4 | ||
| 998 | |g 114590789X |a Osen, Wolfram |m 114590789X:Osen, Wolfram |d 50000 |e 50000PO114590789X |k 0/50000/ |p 3 | ||
| 998 | |g 131798189 |a Song, Mingxia |m 131798189:Song, Mingxia |d 50000 |e 50000PS131798189 |k 0/50000/ |p 2 | ||
| 998 | |g 1076261353 |a Paschen, Annette |m 1076261353:Paschen, Annette |d 50000 |e 50000PP1076261353 |k 0/50000/ |p 1 |x j | ||
| 999 | |a KXP-PPN1745294872 |e 3840958377 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"origin":[{"dateIssuedKey":"2005","dateIssuedDisp":"July 20, 2005"}],"physDesc":[{"extent":"7 S."}],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Annette Paschen, Mingxia Song, Wolfram Osen, Xuan Duc Nguyen, Jan Mueller-Berghaus, Daniela Fink, Nadine Daniel, Mariel Donzeau, Wolfgang Nagel, Harald Kropshofer, and Dirk Schadendorf"]},"id":{"eki":["1745294872"],"doi":["10.1158/1078-0432.CCR-05-0170"]},"person":[{"role":"aut","given":"Annette","display":"Paschen, Annette","family":"Paschen"},{"given":"Mingxia","role":"aut","family":"Song","display":"Song, Mingxia"},{"given":"Wolfram","role":"aut","family":"Osen","display":"Osen, Wolfram"},{"role":"aut","given":"Xuan-Duc","display":"Nguyen, Xuan-Duc","family":"Nguyen"},{"family":"Schadendorf","display":"Schadendorf, Dirk","given":"Dirk","role":"aut"}],"titleAlt":[{"title":"Detection of spontaneous CD 4 + T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice"}],"recId":"1745294872","relHost":[{"id":{"issn":["1557-3265"],"eki":["325489971"],"zdb":["2036787-9"]},"name":{"displayForm":["American Association for Cancer Research"]},"corporate":[{"display":"American Association for Cancer Research","role":"isb"}],"type":{"media":"Online-Ressource","bibl":"periodical"},"physDesc":[{"extent":"Online-Ressource"}],"origin":[{"publisherPlace":"Philadelphia, Pa. [u.a.]","dateIssuedDisp":"1995-","publisher":"AACR","dateIssuedKey":"1995"}],"language":["eng"],"note":["Gesehen am 08.06.2023","Fortsetzung der Druck-Ausgabe"],"title":[{"title":"Clinical cancer research","title_sort":"Clinical cancer research"}],"pubHistory":["1.1995 -"],"recId":"325489971","part":{"extent":"7","year":"2005","pages":"5241-5247","volume":"11","text":"11(2005), 14, Seite 5241-5247","issue":"14"},"disp":"Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic miceClinical cancer research"}],"language":["eng"],"note":["Im Titel ist das Zeichen \"+\" hochgestellt","Gesehen am 22.01.2021"],"title":[{"title_sort":"Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice","title":"Detection of spontaneous CD4+ T-cell responses in melanoma patients against a tyrosinase-related protein-2-derived epitope identified in HLA-DRB1*0301 transgenic mice"}]} | ||
| SRT | |a PASCHENANNDETECTIONO2020 | ||