IL-12 production by human monocyte-derived dendritic cells: looking at the single cell
Dendritic cells (DCs) are under investigation as immunotherapeutic agents in the treatment of cancer and infectious diseases. One of the important factors in skewing the immune response toward clinically beneficial TH1-type immunity is interleukin-12p70. IL-12p70 is synthesized and secreted in respo...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
July/August 2005
|
| In: |
Journal of immunotherapy
Year: 2005, Volume: 28, Issue: 4, Pages: 306-313 |
| ISSN: | 1537-4513 |
| DOI: | 10.1097/01.cji.0000163594.74533.10 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://dx.doi.org/10.1097/01.cji.0000163594.74533.10
|
| Author Notes: | Jan Muller-Berghaus, Walter C. Olson, Rachel A. Moulton, William T. Knapp, Dirk Schadendorf, and Walter J. Storkus |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1745295348 | ||
| 003 | DE-627 | ||
| 005 | 20230426074251.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210122s2005 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1097/01.cji.0000163594.74533.10 |2 doi | |
| 035 | |a (DE-627)1745295348 | ||
| 035 | |a (DE-599)KXP1745295348 | ||
| 035 | |a (OCoLC)1341389048 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Müller-Berghaus, Jan |d 1963- |e VerfasserIn |0 (DE-588)118184660 |0 (DE-627)69474316X |0 (DE-576)180820699 |4 aut | |
| 245 | 1 | 0 | |a IL-12 production by human monocyte-derived dendritic cells |b looking at the single cell |c Jan Muller-Berghaus, Walter C. Olson, Rachel A. Moulton, William T. Knapp, Dirk Schadendorf, and Walter J. Storkus |
| 264 | 1 | |c July/August 2005 | |
| 300 | |a 7 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 22.01.2021 | ||
| 520 | |a Dendritic cells (DCs) are under investigation as immunotherapeutic agents in the treatment of cancer and infectious diseases. One of the important factors in skewing the immune response toward clinically beneficial TH1-type immunity is interleukin-12p70. IL-12p70 is synthesized and secreted in response to inflammatory cytokines, bacterial/viral components, and CD40 ligation. This study investigated the production of IL-12 by DCs at the single-cell level using a sensitive intracellular cytokine flow cytometry-based assay system. The authors observed that immature DCs could be stimulated with several compounds to produce IL-12, but that IL-12 production was a feature of a minority of activated DCs. IL-12+ DCs were characterized as being partially matured (ie, absent or low CD83 expression, with variable expression of CD1a and CD64). Interestingly, activated DCs lacked expression of the CD16 and CD64 Fc gammaR, which may have important implications for exogenous antigen-loading strategies. | ||
| 650 | 4 | |a Antigens, CD | |
| 650 | 4 | |a CD8-Positive T-Lymphocytes | |
| 650 | 4 | |a Cell Adhesion | |
| 650 | 4 | |a Cell Differentiation | |
| 650 | 4 | |a Culture Media, Serum-Free | |
| 650 | 4 | |a Cytokines | |
| 650 | 4 | |a Dendritic Cells | |
| 650 | 4 | |a Humans | |
| 650 | 4 | |a Immunophenotyping | |
| 650 | 4 | |a Interleukin-12 | |
| 650 | 4 | |a Interleukin-12 Subunit p40 | |
| 650 | 4 | |a Leukocytes, Mononuclear | |
| 650 | 4 | |a Lipopolysaccharides | |
| 650 | 4 | |a Lymphocyte Activation | |
| 650 | 4 | |a Monocytes | |
| 650 | 4 | |a Protein Subunits | |
| 650 | 4 | |a Staphylococcus aureus | |
| 700 | 1 | |a Schadendorf, Dirk |d 1960- |e VerfasserIn |0 (DE-588)11142576X |0 (DE-627)499566076 |0 (DE-576)289702275 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of immunotherapy |d Philadelphia, Pa. : Lippincott Williams & Wilkins, 1991 |g 28(2005), 4, Seite 306-313 |h Online-Ressource |w (DE-627)330078151 |w (DE-600)2048797-6 |w (DE-576)100399150 |x 1537-4513 |7 nnas |a IL-12 production by human monocyte-derived dendritic cells looking at the single cell |
| 773 | 1 | 8 | |g volume:28 |g year:2005 |g number:4 |g pages:306-313 |g extent:7 |a IL-12 production by human monocyte-derived dendritic cells looking at the single cell |
| 856 | 4 | 0 | |u https://dx.doi.org/10.1097/01.cji.0000163594.74533.10 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210122 | ||
| 993 | |a Article | ||
| 994 | |a 2005 | ||
| 998 | |g 11142576X |a Schadendorf, Dirk |m 11142576X:Schadendorf, Dirk |d 50000 |e 50000PS11142576X |k 0/50000/ |p 5 | ||
| 999 | |a KXP-PPN1745295348 |e 3840977339 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"note":["Gesehen am 22.01.2021"],"recId":"1745295348","id":{"eki":["1745295348"],"doi":["10.1097/01.cji.0000163594.74533.10"]},"title":[{"title":"IL-12 production by human monocyte-derived dendritic cells","title_sort":"IL-12 production by human monocyte-derived dendritic cells","subtitle":"looking at the single cell"}],"origin":[{"dateIssuedDisp":"July/August 2005","dateIssuedKey":"2005"}],"type":{"media":"Online-Ressource","bibl":"article-journal"},"language":["eng"],"relHost":[{"pubHistory":["10.1991 -"],"recId":"330078151","part":{"volume":"28","issue":"4","text":"28(2005), 4, Seite 306-313","pages":"306-313","extent":"7","year":"2005"},"physDesc":[{"extent":"Online-Ressource"}],"language":["eng"],"origin":[{"dateIssuedKey":"1991","publisherPlace":"Philadelphia, Pa. ; [Erscheinungsort nicht ermittelbar]","dateIssuedDisp":"1991-","publisher":"Lippincott Williams & Wilkins ; Ovid"}],"type":{"bibl":"periodical","media":"Online-Ressource"},"title":[{"title":"Journal of immunotherapy","title_sort":"Journal of immunotherapy","subtitle":"official journal of the Society for Biological Therapy"}],"id":{"issn":["1537-4513"],"zdb":["2048797-6"],"eki":["330078151"]},"note":["Gesehen am 24.11.20"],"disp":"IL-12 production by human monocyte-derived dendritic cells looking at the single cellJournal of immunotherapy"}],"physDesc":[{"extent":"7 S."}],"person":[{"display":"Müller-Berghaus, Jan","role":"aut","given":"Jan","family":"Müller-Berghaus","roleDisplay":"VerfasserIn"},{"family":"Schadendorf","roleDisplay":"VerfasserIn","given":"Dirk","role":"aut","display":"Schadendorf, Dirk"}],"name":{"displayForm":["Jan Muller-Berghaus, Walter C. Olson, Rachel A. Moulton, William T. Knapp, Dirk Schadendorf, and Walter J. Storkus"]}} | ||
| SRT | |a MUELLERBERIL12PRODUC2005 | ||