Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteins

Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet a systematic investigation of the composition and dynamics of the OSN protein network specifically on chromatin is still missing. Here we have developed a method combining C...

Full description

Saved in:
Bibliographic Details
Main Authors: Rafiee, Mahmoud-Reza (Author) , Girardot, Charles (Author) , Sigismondo, Gianluca (Author) , Krijgsveld, Jeroen (Author)
Format: Article (Journal)
Language:English
Published: October 20, 2016
In: Molecular cell
Year: 2016, Volume: 64, Issue: 3, Pages: 624-635
ISSN:1097-4164
DOI:10.1016/j.molcel.2016.09.019
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.molcel.2016.09.019
Verlag, lizenzpflichtig, Volltext: http://www.sciencedirect.com/science/article/pii/S1097276516305688
Get full text
Author Notes:Mahmoud-Reza Rafiee, Charles Girardot, Gianluca Sigismondo, and Jeroen Krijgsveld

MARC

LEADER 00000caa a2200000 c 4500
001 1745752692
003 DE-627
005 20220819085346.0
007 cr uuu---uuuuu
008 210127s2016 xx |||||o 00| ||eng c
024 7 |a 10.1016/j.molcel.2016.09.019  |2 doi 
035 |a (DE-627)1745752692 
035 |a (DE-599)KXP1745752692 
035 |a (OCoLC)1341389690 
040 |a DE-627  |b ger  |c DE-627  |e rda 
041 |a eng 
084 |a 33  |2 sdnb 
100 1 |a Rafiee, Mahmoud-Reza  |e VerfasserIn  |0 (DE-588)1081952792  |0 (DE-627)846907798  |0 (DE-576)454975457  |4 aut 
245 1 0 |a Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteins  |c Mahmoud-Reza Rafiee, Charles Girardot, Gianluca Sigismondo, and Jeroen Krijgsveld 
264 1 |c October 20, 2016 
300 |a 12 
336 |a Text  |b txt  |2 rdacontent 
337 |a Computermedien  |b c  |2 rdamedia 
338 |a Online-Ressource  |b cr  |2 rdacarrier 
500 |a Gesehen am 27.01.2021 
520 |a Maintenance of pluripotency is regulated by a network of transcription factors coordinated by Oct4, Sox2, and Nanog (OSN), yet a systematic investigation of the composition and dynamics of the OSN protein network specifically on chromatin is still missing. Here we have developed a method combining ChIP with selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry to identify chromatin-bound partners of a protein of interest. ChIP-SICAP in mouse embryonic stem cells (ESCs) identified over 400 proteins associating with OSN, including several whose interaction depends on the pluripotent state. Trim24, a previously unrecognized protein in the network, converges with OSN on multiple enhancers and suppresses the expression of developmental genes while activating cell cycle genes. Consistently, Trim24 significantly improved efficiency of cellular reprogramming, demonstrating its direct functionality in establishing pluripotency. Collectively, ChIP-SICAP provides a powerful tool to decode chromatin protein composition, further enhanced by its integrative capacity to perform ChIP-seq. 
650 4 |a biotinylation 
650 4 |a chromatin 
650 4 |a embryonic stem cells 
650 4 |a pluripotency 
650 4 |a protein interactions 
650 4 |a proteomics 
650 4 |a reprogramming 
700 1 |a Girardot, Charles  |e VerfasserIn  |0 (DE-588)1131811356  |0 (DE-627)886554071  |0 (DE-576)488503361  |4 aut 
700 1 |a Sigismondo, Gianluca  |e VerfasserIn  |0 (DE-588)1162971843  |0 (DE-627)102706504X  |0 (DE-576)507717538  |4 aut 
700 1 |a Krijgsveld, Jeroen  |e VerfasserIn  |0 (DE-588)1163292125  |0 (DE-627)1027502032  |0 (DE-576)507882474  |4 aut 
773 0 8 |i Enthalten in  |t Molecular cell  |d [Cambridge, Mass.] : Cell Press, 1997  |g 64(2016), 3, Seite 624-635  |h Online-Ressource  |w (DE-627)320416097  |w (DE-600)2001948-8  |w (DE-576)090881362  |x 1097-4164  |7 nnas  |a Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteins 
773 1 8 |g volume:64  |g year:2016  |g number:3  |g pages:624-635  |g extent:12  |a Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteins 
856 4 0 |u https://doi.org/10.1016/j.molcel.2016.09.019  |x Verlag  |x Resolving-System  |z lizenzpflichtig  |3 Volltext 
856 4 0 |u http://www.sciencedirect.com/science/article/pii/S1097276516305688  |x Verlag  |z lizenzpflichtig  |3 Volltext 
951 |a AR 
992 |a 20210127 
993 |a Article 
994 |a 2016 
998 |g 1163292125  |a Krijgsveld, Jeroen  |m 1163292125:Krijgsveld, Jeroen  |d 50000  |d 700000  |d 718000  |e 50000PK1163292125  |e 700000PK1163292125  |e 718000PK1163292125  |k 0/50000/  |k 0/700000/  |k 1/700000/718000/  |p 4  |y j 
998 |g 1162971843  |a Sigismondo, Gianluca  |m 1162971843:Sigismondo, Gianluca  |d 700000  |d 718000  |e 700000PS1162971843  |e 718000PS1162971843  |k 0/700000/  |k 1/700000/718000/  |p 3 
998 |g 1081952792  |a Rafiee, Mahmoud-Reza  |m 1081952792:Rafiee, Mahmoud-Reza  |d 910000  |d 911700  |e 910000PR1081952792  |e 911700PR1081952792  |k 0/910000/  |k 1/910000/911700/  |p 1  |x j 
999 |a KXP-PPN1745752692  |e 384331750X 
BIB |a Y 
SER |a journal 
JSO |a {"origin":[{"dateIssuedDisp":"October 20, 2016","dateIssuedKey":"2016"}],"name":{"displayForm":["Mahmoud-Reza Rafiee, Charles Girardot, Gianluca Sigismondo, and Jeroen Krijgsveld"]},"recId":"1745752692","note":["Gesehen am 27.01.2021"],"physDesc":[{"extent":"12 S."}],"id":{"doi":["10.1016/j.molcel.2016.09.019"],"eki":["1745752692"]},"type":{"media":"Online-Ressource","bibl":"article-journal"},"title":[{"title":"Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteins","title_sort":"Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteins"}],"relHost":[{"recId":"320416097","origin":[{"publisherPlace":"[Cambridge, Mass.] ; New York, NY","dateIssuedKey":"1997","dateIssuedDisp":"1997-","publisher":"Cell Press ; Elsevier"}],"pubHistory":["1.1997/98 -"],"part":{"extent":"12","year":"2016","volume":"64","text":"64(2016), 3, Seite 624-635","issue":"3","pages":"624-635"},"type":{"media":"Online-Ressource","bibl":"periodical"},"id":{"eki":["320416097"],"issn":["1097-4164"],"zdb":["2001948-8"]},"disp":"Expanding the circuitry of pluripotency by selective isolation of chromatin-associated proteinsMolecular cell","note":["Gesehen am 01.08.25"],"language":["eng"],"title":[{"title":"Molecular cell","title_sort":"Molecular cell"}],"physDesc":[{"extent":"Online-Ressource"}]}],"language":["eng"],"person":[{"role":"aut","display":"Rafiee, Mahmoud-Reza","given":"Mahmoud-Reza","family":"Rafiee"},{"family":"Girardot","given":"Charles","display":"Girardot, Charles","role":"aut"},{"role":"aut","display":"Sigismondo, Gianluca","given":"Gianluca","family":"Sigismondo"},{"given":"Jeroen","family":"Krijgsveld","role":"aut","display":"Krijgsveld, Jeroen"}]} 
SRT |a RAFIEEMAHMEXPANDINGT2020