Reaction-diffusion modelling for microphysiometry on cellular specimens
Using modeling and simulation, we quantify the influence of spatiotemporal dynamics on the accuracy of data obtained from sensors placed in microscaled reaction volumes. The model refers to cellular reaction (i.e. proton extrusion and oxygen consumption) in complex, buffering solutions. Whole cells...
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2013
|
| In: |
Medical & biological engineering & computing
Year: 2012, Jahrgang: 51, Heft: 4, Pages: 387-395 |
| ISSN: | 1741-0444 |
| DOI: | 10.1007/s11517-012-1007-4 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11517-012-1007-4
|
| Verfasserangaben: | Daniel Grundl, Xiaorui Zhang, Safa Messaoud, Cornelia Pfister, Franz Demmel, Mario S. Mommer, Bernhard Wolf, Martin Brischwein |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 1746008539 | ||
| 003 | DE-627 | ||
| 005 | 20230426075043.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210129r20132012xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s11517-012-1007-4 |2 doi | |
| 035 | |a (DE-627)1746008539 | ||
| 035 | |a (DE-599)KXP1746008539 | ||
| 035 | |a (OCoLC)1341389990 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Grundl, Daniel |e VerfasserIn |0 (DE-588)1227363753 |0 (DE-627)1748391356 |4 aut | |
| 245 | 1 | 0 | |a Reaction-diffusion modelling for microphysiometry on cellular specimens |c Daniel Grundl, Xiaorui Zhang, Safa Messaoud, Cornelia Pfister, Franz Demmel, Mario S. Mommer, Bernhard Wolf, Martin Brischwein |
| 264 | 1 | |c 2013 | |
| 300 | |a 9 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Published online: 1 December 2012 | ||
| 500 | |a Gesehen am 16.02.2021 | ||
| 520 | |a Using modeling and simulation, we quantify the influence of spatiotemporal dynamics on the accuracy of data obtained from sensors placed in microscaled reaction volumes. The model refers to cellular reaction (i.e. proton extrusion and oxygen consumption) in complex, buffering solutions. Whole cells or viable tissues cultured in such devices are monitored in real time with integrated sensors for pH and dissolved oxygen. A 3D finite element model of diffusion and metabolic reaction was set up. With respect to pH, the effect of buffering species on proton diffusion is analysed in detail. To account for the delayed time response of real sensors, the sensor impulse response time was implemented by linear convolution. A validation of the model has been achieved by an electrochemical approach. The model reveals significant deviations of measured pH and O2, and values of these parameters actually occurring at different sites of the cell culture volume. It is applicable to any setting of (bio-) sensors involving reaction and diffusion of dissolved gases and particularly H+ ions in buffered solutions. | ||
| 534 | |c 2012 | ||
| 700 | 1 | |a Mommer, Mario Salvador |d 1973- |e VerfasserIn |0 (DE-588)130244481 |0 (DE-627)495827819 |0 (DE-576)29808449X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Medical & biological engineering & computing |d Cham : Springer Nature, 1963 |g 51(2013), 4, Seite 387-395 |h Online-Ressource |w (DE-627)331747456 |w (DE-600)2052667-2 |w (DE-576)273651145 |x 1741-0444 |7 nnas |a Reaction-diffusion modelling for microphysiometry on cellular specimens |
| 773 | 1 | 8 | |g volume:51 |g year:2013 |g number:4 |g pages:387-395 |g extent:9 |a Reaction-diffusion modelling for microphysiometry on cellular specimens |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s11517-012-1007-4 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210129 | ||
| 993 | |a Article | ||
| 994 | |a 2013 | ||
| 998 | |g 130244481 |a Mommer, Mario Salvador |m 130244481:Mommer, Mario Salvador |d 700000 |d 708000 |e 700000PM130244481 |e 708000PM130244481 |k 0/700000/ |k 1/700000/708000/ |p 6 | ||
| 999 | |a KXP-PPN1746008539 |e 3844510141 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"relHost":[{"title":[{"title_sort":"Medical & biological engineering & computing","title":"Medical & biological engineering & computing"}],"pubHistory":["1.1963 -"],"titleAlt":[{"title":"Medical and biological engineering & computing"},{"title":"Medical electronics and biological engineering"},{"title":"Medical & biological engineering"}],"part":{"extent":"9","text":"51(2013), 4, Seite 387-395","volume":"51","pages":"387-395","issue":"4","year":"2013"},"disp":"Reaction-diffusion modelling for microphysiometry on cellular specimensMedical & biological engineering & computing","note":["Gesehen am 27.05.2022"],"type":{"media":"Online-Ressource","bibl":"periodical"},"recId":"331747456","language":["eng"],"corporate":[{"roleDisplay":"Herausgebendes Organ","display":"International Federation for Medical and Biological Engineering","role":"isb"}],"origin":[{"dateIssuedDisp":"1963-","publisher":"Springer Nature","dateIssuedKey":"1963","publisherPlace":"Cham"}],"id":{"eki":["331747456"],"zdb":["2052667-2"],"issn":["1741-0444"]},"name":{"displayForm":["International Federation for Medical and Biological Engineering"]},"physDesc":[{"extent":"Online-Ressource"}]}],"physDesc":[{"extent":"9 S."}],"id":{"doi":["10.1007/s11517-012-1007-4"],"eki":["1746008539"]},"origin":[{"dateIssuedKey":"2013","dateIssuedDisp":"2013"}],"name":{"displayForm":["Daniel Grundl, Xiaorui Zhang, Safa Messaoud, Cornelia Pfister, Franz Demmel, Mario S. Mommer, Bernhard Wolf, Martin Brischwein"]},"language":["eng"],"recId":"1746008539","type":{"bibl":"article-journal","media":"Online-Ressource"},"note":["Published online: 1 December 2012","Gesehen am 16.02.2021"],"title":[{"title_sort":"Reaction-diffusion modelling for microphysiometry on cellular specimens","title":"Reaction-diffusion modelling for microphysiometry on cellular specimens"}],"person":[{"roleDisplay":"VerfasserIn","display":"Grundl, Daniel","role":"aut","family":"Grundl","given":"Daniel"},{"roleDisplay":"VerfasserIn","display":"Mommer, Mario Salvador","role":"aut","family":"Mommer","given":"Mario Salvador"}]} | ||
| SRT | |a GRUNDLDANIREACTIONDI2013 | ||