The role of integrin-linked kinase in the molecular architecture of focal adhesions
Skip to Next Section - Integrin-mediated focal adhesions (FAs) are large, multi-protein complexes that link the actin cytoskeleton to the extracellular matrix and take part in adhesion-mediated signaling. These adhesions are highly complex and diverse at the molecular level; thus, assigning particul...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 September 2013
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| In: |
Journal of cell science
Year: 2013, Jahrgang: 126, Heft: 18, Pages: 4099-4107 |
| ISSN: | 1477-9137 |
| DOI: | 10.1242/jcs.120295 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1242/jcs.120295 Verlag, lizenzpflichtig, Volltext: https://jcs.biologists.org/content/126/18/4099 
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| Verfasserangaben: | Nadav Elad, Tova Volberg, Israel Patla, Vera Hirschfeld-Warneken, Carsten Grashoff, Joachim P. Spatz, Reinhard Fässler, Benjamin Geiger and Ohad Medalia |
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| 520 | |a Skip to Next Section - Integrin-mediated focal adhesions (FAs) are large, multi-protein complexes that link the actin cytoskeleton to the extracellular matrix and take part in adhesion-mediated signaling. These adhesions are highly complex and diverse at the molecular level; thus, assigning particular structural or signaling functions to specific components is highly challenging. Here, we combined functional, structural and biophysical approaches to assess the role of a major FA component, namely, integrin-linked kinase (ILK), in adhesion formation. We show here that ILK plays a key role in the formation of focal complexes, early forms of integrin adhesions, and confirm its involvement in the assembly of fibronectin-bound fibrillar adhesions. Examination of ILK-null fibroblasts by cryo-electron tomography pointed to major structural changes in their FAs, manifested as disarray of the associated actin filaments and an increase in the packing density of FA-related particles. Interestingly, adhesion of the mutant cells to the substrate required a higher ligand density than in control cells. These data indicate that ILK has a key role in integrin adhesion assembly and sub-structure, and in the regulation of the FA-associated cytoskeleton. | ||
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