Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol Pool
<p>Exposure of T cells to the macrophage products hydrogen peroxide (HP) or l-lactate (LAC) was previously shown to enhance IL-2 production and to modulate glutathione (GSH) status. We now found that 50 μM HP and 30 mM LAC enhanced strongly the transcription from the IL-2 promoter in Jurkat T...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
October 15, 2000
|
| In: |
The journal of immunology
Year: 2000, Jahrgang: 165, Heft: 8, Pages: 4319-4328 |
| ISSN: | 1550-6606 |
| DOI: | 10.4049/jimmunol.165.8.4319 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.4049/jimmunol.165.8.4319 Verlag, lizenzpflichtig, Volltext: https://www.jimmunol.org/content/165/8/4319 
|
| Verfasserangaben: | Steffen P. Hehner, Raoul Breitkreutz, George Shubinsky, Heike Unsoeld, Klaus Schulze-Osthoff, M. Lienhard Schmitz, and Wulf Dröge |
MARC
| LEADER | 00000caa a2200000 c 4500 | ||
|---|---|---|---|
| 001 | 174720627X | ||
| 003 | DE-627 | ||
| 005 | 20220819093916.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210203s2000 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.4049/jimmunol.165.8.4319 |2 doi | |
| 035 | |a (DE-627)174720627X | ||
| 035 | |a (DE-599)KXP174720627X | ||
| 035 | |a (OCoLC)1341390231 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rda | ||
| 041 | |a eng | ||
| 084 | |a 33 |2 sdnb | ||
| 100 | 1 | |a Hehner, Steffen Peter |d 1971- |e VerfasserIn |0 (DE-588)122028260 |0 (DE-627)081691718 |0 (DE-576)182356566 |4 aut | |
| 245 | 1 | 0 | |a Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol Pool |c Steffen P. Hehner, Raoul Breitkreutz, George Shubinsky, Heike Unsoeld, Klaus Schulze-Osthoff, M. Lienhard Schmitz, and Wulf Dröge |
| 264 | 1 | |c October 15, 2000 | |
| 300 | |a 11 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Gesehen am 03.02.2021 | ||
| 520 | |a <p>Exposure of T cells to the macrophage products hydrogen peroxide (HP) or l-lactate (LAC) was previously shown to enhance IL-2 production and to modulate glutathione (GSH) status. We now found that 50 μM HP and 30 mM LAC enhanced strongly the transcription from the IL-2 promoter in Jurkat T cells after stimulation with anti-CD28 together with or without anti-CD3 but not with anti-CD3 Abs alone. Therefore, we used anti-CD3 plus anti-CD28-stimulated cells to investigate the effect of the GSH reductase inhibitor 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) on the signal cascade. BCNU enhanced the transcription to a similar extent as HP or LAC. Lowering the intracellular GSH/GSH disulfide ratio by BCNU, HP, or NO resulted in all cases in the fulminant enhancement of Jun-N-terminal kinase and p38 mitogen-activated protein kinase but not extracellular signal-regulated kinase 1/2. Jun-N-terminal kinase and NF-κB activation was enhanced through pathways involving Rac, Vav1, PKCΘ, p56<sup>lck</sup>, p59<sup>fyn</sup>, and IκB kinases. In a cell-free system, the autophosphorylation of rFyn was stimulated by GSH disulfide but not by HP. These findings suggest that the oxidation of the cellular thiol pool may play a role as an amplifying mechanism for TCR/CD3 signals in immune responses.</p> | ||
| 700 | 1 | |a Schulze-Osthoff, Klaus |e VerfasserIn |0 (DE-588)111112549X |0 (DE-627)865517703 |0 (DE-576)173021980 |4 aut | |
| 700 | 1 | |a Dröge, Wulf |d -2011 |e VerfasserIn |0 (DE-588)1178587789 |0 (DE-627)1049465539 |0 (DE-576)517837528 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The journal of immunology |d Rockville, Md. : American Association of Immunologists, 1916 |g 165(2000), 8, Seite 4319-4328 |h Online-Ressource |w (DE-627)269535217 |w (DE-600)1475085-5 |w (DE-576)079963838 |x 1550-6606 |7 nnas |a Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol Pool |
| 773 | 1 | 8 | |g volume:165 |g year:2000 |g number:8 |g pages:4319-4328 |g extent:11 |a Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol Pool |
| 856 | 4 | 0 | |u https://doi.org/10.4049/jimmunol.165.8.4319 |x Verlag |x Resolving-System |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u https://www.jimmunol.org/content/165/8/4319 |x Verlag |z lizenzpflichtig |3 Volltext |
| 951 | |a AR | ||
| 992 | |a 20210203 | ||
| 993 | |a Article | ||
| 994 | |a 2000 | ||
| 998 | |g 1178587789 |a Dröge, Wulf |m 1178587789:Dröge, Wulf |d 50000 |e 50000PD1178587789 |k 0/50000/ |p 7 |y j | ||
| 998 | |g 111112549X |a Schulze-Osthoff, Klaus |m 111112549X:Schulze-Osthoff, Klaus |d 50000 |e 50000PS111112549X |k 0/50000/ |p 5 | ||
| 999 | |a KXP-PPN174720627X |e 3848324067 | ||
| BIB | |a Y | ||
| SER | |a journal | ||
| JSO | |a {"person":[{"given":"Steffen Peter","family":"Hehner","role":"aut","roleDisplay":"VerfasserIn","display":"Hehner, Steffen Peter"},{"given":"Klaus","family":"Schulze-Osthoff","role":"aut","display":"Schulze-Osthoff, Klaus","roleDisplay":"VerfasserIn"},{"family":"Dröge","given":"Wulf","display":"Dröge, Wulf","roleDisplay":"VerfasserIn","role":"aut"}],"title":[{"title":"Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol Pool","title_sort":"Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol Pool"}],"recId":"174720627X","language":["eng"],"note":["Gesehen am 03.02.2021"],"type":{"bibl":"article-journal","media":"Online-Ressource"},"name":{"displayForm":["Steffen P. Hehner, Raoul Breitkreutz, George Shubinsky, Heike Unsoeld, Klaus Schulze-Osthoff, M. Lienhard Schmitz, and Wulf Dröge"]},"id":{"doi":["10.4049/jimmunol.165.8.4319"],"eki":["174720627X"]},"origin":[{"dateIssuedDisp":"October 15, 2000","dateIssuedKey":"2000"}],"relHost":[{"origin":[{"dateIssuedDisp":"2025-","dateIssuedKey":"2025","publisher":"Oxford University Press ; Soc. ; American Association of Immunologists","publisherPlace":"Oxford ; Bethesda, Md. ; Rockville, Md."}],"id":{"issn":["1550-6606"],"zdb":["1475085-5"],"eki":["269535217"]},"name":{"displayForm":["American Association of Immunologists"]},"physDesc":[{"extent":"Online-Ressource"}],"title":[{"title":"The journal of immunology","title_sort":"journal of immunology"}],"pubHistory":["1.1916 -"],"part":{"extent":"11","text":"165(2000), 8, Seite 4319-4328","volume":"165","issue":"8","pages":"4319-4328","year":"2000"},"type":{"media":"Online-Ressource","bibl":"periodical"},"disp":"Enhancement of T cell receptor signaling by a mild oxidative shift in the intracellular thiol PoolThe journal of immunology","note":["Gesehen am 18.08.2025"],"recId":"269535217","corporate":[{"display":"American Association of Immunologists","roleDisplay":"Herausgebendes Organ","role":"isb"}],"language":["eng"]}],"physDesc":[{"extent":"11 S."}]} | ||
| SRT | |a HEHNERSTEFENHANCEMEN1520 | ||